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Citations for Promega :
4201 - 4250 of 4685 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega, Madison, WI, USA). A second incubation with the same amount of trypsin (5 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... a total of 1□μg of RNA for each sample was reverse-transcribed to cDNA using reverse-transcription kits (Promega, Madison, WI, USA), and real-time quantitative reverse transcription (qRT)-PCR was performed on a 7500 real-time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... To this was added: 1 µl of EcoRV-digested pBlueScript KS II+ (∼100 ng) that had been treated with calf intestinal phosphatase (Promega Corp., Madison, WI), and 0.1 µl of T4 DNA ligase (NEB) ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
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bioRxiv - Pathology 2021Quote: ... benthamiana leaves were collected at 48 h after infiltration and then incubated with 1 mM luciferin substrate (catalog no. N1110; Promega Biotech, Beijing, China). LUC images were captured with a Tanon-5200 Multi Chemiluminescent Imaging System (Tanon ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellets were reconstituted and digested with endoproteinase Lys-C (Alpha Laboratories, UK) for 1 hour at room temperature and trypsin (Promega, Madison, WI, USA) overnight at 35°C.
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bioRxiv - Biochemistry 2022Quote: ... transfer plasmid was co-transfected with GAG/POL and VSVG plasmids into HEK293T or 293FT cells using FuGENE HD (Promega, 3.33:1 FuGENE:DNA) and the medium was replaced 6–8 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... on the day before transfection and were transfected with 1 μg of plasmid DNA per well using FuGENE-HD reagent (Promega, product number E2312) per manufacturer guidelines ...
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bioRxiv - Developmental Biology 2022Quote: Samples for CaTS-RNAseq were collected in the same way as for CaTS-ATAC but all buffers were supplemented with 1 µL/ml of RNasin Plus (Promega, Cat. No. N2615). After fixation and quenching ...
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bioRxiv - Immunology 2020Quote: ... washed beads were then resuspended in 35 μL of ammonium bicarb buffer to which 1 μL sequencing grade porcine trypsin was added (Promega, Cat. No. V5111). Immune complexes were digested on bead for 1 hr at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The samples after pull-down assay and co-immunoprecipitation were also digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight in the same manner ...
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bioRxiv - Microbiology 2022Quote: ... the aqueous phase was added to 1 volume of 70% ethanol and loaded on ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B, anti-rabbit IgG HRP conjugate Promega #W4011, 1:3000) and chemiluminescence was detected using Western Bright ECL spray (Advansta ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s recommended protocol for oligo dT-primed synthesis ...
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bioRxiv - Pathology 2020Quote: ... The partial sequence encoding the nucleoprotein from HPWMoV was amplified using specific primers (Table 1) from two-fold diluted cDNA with GoTaq® Flexi DNA polymerase (Promega, WI, USA) with the following reaction conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... and digested with trypsin at an enzyme-to-substrate ratio of 1:50 (wt/wt, modified sequencing grade, Promega, Madison, Wisconsin. United States) for 16 hours at 30°C ...
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bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded on poly-lysine coated glass coverslips at a concentration of 1 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) two days before measurement.
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bioRxiv - Biochemistry 2021Quote: ... Tryptic samples were subsequently diluted to 2M urea in 100mM Tris/HCl pH 8 and digested over night at 37°C (Promega, enzyme:protein 1:100). Samples were adjusted to pH 2 using 10% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2021Quote: Isolated protein aggregate fraction was solubilized for 1 h at room temperature with the use of 10 µl 0.1 % ProteaseMAX™ surfactant (Promega, cat. no. V2072) in 50 mM ammonium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s protocol for oligo dT-primed synthesis ...
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bioRxiv - Microbiology 2019Quote: ... The primer pairs used in dsRNA synthesis are shown in Supplemental Table 1.1 μg PCR product was used as the template for dsRNA synthesis utilizing the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The dsRNA was isopropanol-precipitated ...
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bioRxiv - Genomics 2021Quote: ... We added tagmentation buffer (1x TD buffer, 0.015 µL TTE Mix V50 [Vazyme, #TD501], 0.625x protease inhibitor cocktail [Promega, #G6521], and 1 mM MgCl2) to reach a volume of 10 µL per well and then incubated the plates at 55 °C for 1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... was amplified by PCR using tnnc1a-CDS primers (Table 1) and cloned into the pGEM®-T Easy Vector Systems (Promega, catalog #: A1360). A tnnc1a cDNA clone was confirmed by sequencing and used to make digoxigenin-labeled riboprobes with the Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription was performed at 70 °C for 5 min and 42 °C for 60 min with 1 µg total RNA using ImProm-II reverse transcriptase (Promega, Madison, WI, USA) and 250 ng oligo(dT)12-18 primers (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Developmental Biology 2024Quote: ... buffer (containing 10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 1% BSA, 0.1% Tween, 1mM DTT, 1U/ul RNaseIn from Promega, 0.1% NP40, and 0.01% Digitonin) and incubated on ice for 4 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2024Quote: ... cells was transfected with 250ng of each of 2 pX330 constructs (containing Cas9 and 1 sgRNA) and 500 ng of the plasmid template with FuGene HD transfection reagent (Promega Corporation, Madison, WI) in the recommended conditions ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: The cytotoxicity of all compounds (except ARINA-1 that interferes with the assay) was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Cat. # G1780), which measures LDH enzyme release ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-methionine-labeled Ctb or its derivatives were produced in coupled in vitro transcription and translation reaction (IVTT, at 30 °C for 1 h) using TNT Quick Coupled Transcription/Translation System (cat#L1170, Promega, Madison, WI, USA). Prey proteins were diluted in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G, US], 0.2 U/µl RNAsin Plus RNase inhibitor [Promega, N2615, US]).
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Neuroscience 2024Quote: ... For experiments cells were plated onto poly-D-lysine coated glass coverslips supplemented with 1 μM all-trans retinal and transfected using FuGENE® HD (Promega, Madison, USA) the next day ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of RNA was subjected to reverse transcription using M-MLV enzyme (Promega #M1705), dNTP mix 100 mM each (BLIRT #RP65 ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Genetics 2019Quote: ... following manufacturer’s protocol at 50°C in the presence of 2 U/μl RNasin (Promega). RT negative controls in which the reverse transcriptase enzyme was omitted were set up in parallel ...
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bioRxiv - Molecular Biology 2019Quote: ... The linearized plasmid was treated with 2 units of shrimp alkaline phosphatase (Promega, Madison, USA) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of DNA template in each reaction ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of Nichols DNA template ...