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Citations for Promega :
4301 - 4350 of 4685 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were separated by 12% Tris–Glycine– SDS–PAGE and the gel was electroblotted to a PVDF membrane (0.2 µm) followed by immunodetection using Anti-HaloTag® Monoclonal Antibody (1:5000) (Promega, Madison, Wisconsin, United States) in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... the presence of other main circulating arboviruses was evaluated by RT-qPCR using GoTaq® 1-Step RT-qPCR System (Promega, Madison, WI, USA). Specific diagnosis primers were used for Zika [30] ...
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bioRxiv - Biochemistry 2024Quote: ... the amplified PCR products were digested using the corresponding restriction enzymes (Supplementary Table 1) and then ligated into similarly digested pYES2 using LigaFast Rapid Ligation System (Promega Corporation, Madison, WI, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... or SL1-deleted SARS-CoV-2 5’ UTR luciferase reporter plasmid using FuGENE Transfection Reagent (Promega). Luciferase assays were performed using the Luciferase Assay System (Promega) ...
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of culture was added to 50 μl of 1X Passive Lysis Buffer (Promega #E1941) which was aliquoted in a reader plate (Corning #CLS3912) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 or MIA PaCa-2 cells were transfected with different AGO2 constructs using Fugene HD (Promega) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... 2 μg of each of these reporter plasmids along with 100 ng of pRL-TK (Promega), a Renilla luciferase expression plasmid to control for transfection efficiency ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg RNA was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega, Madison, USA) and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega, USA) and used to amplify part of the mitochondrial cytochrome oxidase 1 (CO1 ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: ... JAK2 and MAP3K2 are cloned downstream of Renilla luciferase (Rluc) in the modified psiCHECK-2 (Promega) plasmid p5522 using the Gateway cloning technology (m Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... hepatica calmodulin 2 (fhcam2, AM412547) were generated using the T7 RiboMAX™ Express RNAi System (Promega) with primers listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... BT-549 and HCC38 cells were transfected with both plasmids simultaneously (2 μg) using FuGene (Promega) according to the manufacturer’s instructions ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Microbiology 2020Quote: ... incubated in the dark for 2 mins and read on a Glomax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2020Quote: ... 18h (SARS-CoV-2) or 24h (SARS-CoV) later by adding 1μM of Coelenterazine-H (Promega) at 1:400 dilution in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with 2 μg DNA using FuGENE® HD Transfection Reagent (REF E2311, Promega). After 24 hours ...
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bioRxiv - Microbiology 2020Quote: Supernatants were taken from cell medium and mixed with equal volume of 2 ×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Fugene HD (Fugene HD [μL]:DNA [μg]:DMEM [μL] = 5:2:200;Promega, Madison, WI, USA) was used for transient transfection ...
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bioRxiv - Neuroscience 2021Quote: ... For RNA retrotranscription and RT-PCR it was used GoTaq 2-Step RT qPCR System (Promega) according to the manufacturer’s instructions and it was performed using Mic qPCR Cycler (BioMolecular Systems ...
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bioRxiv - Microbiology 2021Quote: Supernatants were taken from cell medium and mixed with equal volumes of 2×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... by quickly mixing 2 μL of above refolding mixture with 50 μL of luciferase substrate (Promega). The activity of unheated luciferase in buffer B was set as 100%.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of each purified plasmid was used as input in a 50 μl TnT (Promega) reaction incubated at 25 °C for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: The pSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized (using 2 µg RNA) using the Go Script™ Reverse Transcriptase Kit (Promega) following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of exon 2 of the NR2F2 gene was performed using PCR Master Mix (Promega; primer pair is listed in Appendix Table S1) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were successively lysed and assayed by a 2-DG uptake assay kit (J1341, Promega) according to the instruction ...
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bioRxiv - Physiology 2023Quote: 2 μg RNA was converted to first–strand cDNA by using reverse transcriptase (Promega, Madison, WI) and Oligo–D (T ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... described in Supplemental Table 2 were used for amplification with GoTaq G2 polymerase (Promega, WI, USA). Amplification was performed by 35 cycles PCR each consisting of 94°C for 30 sec ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in a medium containing 2 nM Janelia Fluor 646 (JF646) HaloTag ligand (Promega) and 0.5 μg/mL Hoechst33342 (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA samples were additionally pre-treated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3’ UTR fragment was digested with Xba I and inserted downstream of the firefly luciferase gene in pGL3-control (Promega, Madison, WI, USA), yielding pGL3covi-3UTR_Luc ...
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bioRxiv - Genetics 2019Quote: Buccal cell samples were collected and prepared as for the standard processing method with the exception that 3 μl 5 x AmpSolution™ (Promega Corporation, USA), was added to the PCR reagents in place of low TE buffer ...