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Citations for Promega :
4001 - 4050 of 4685 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Biochemistry 2021Quote: ... Genomic regions flanking the CRISPR target sites were PCR amplified with designed primers (Supplemental Table 3) using GoTaq DNA polymerase (Promega) and sent for Sanger sequencing to determine the insertion and deletion errors generated by CRISPR-Cas9 system in exon 2 of PARKIN ...
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bioRxiv - Biochemistry 2020Quote: ... protein was modified with an poly(A)25 insertion at the 3’ end of the gene within the pRL-null vector (Promega). The gene encoding the NanoLuc luciferase was transferred from the pNL1.1 vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was probed with a 400 bp 32P-labelled PCR product of the TbCls 3’UTR generated with the prime-a-gene labelling system (Promega). Primers used for PCR were ...
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bioRxiv - Bioengineering 2020Quote: ... Reverse transcription into cDNA was done from 3 μg of RNA by using 500 μg/mL random hexamers (Promega, Switzerland) and 0.5 μL of 200 UI/mL SuperScript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity in primary neuronal cells was measured using ApoTox-Glo Triplex Assay kit (Promega, Madison, WI, USA) as per the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of cells was measured after 3 days incubation using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... To remove unwanted 3’phosphate groups from bound RNA fragments in both CLASH and FLASH the complexes were treated with TSAP phosphatase (Promega) using provided buffer for 40 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1 cells were treated with different concentrations of RG7834 for 3 days and viability was measured using Cell Titer Glo (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Apoptosis induction by T3DC and inhibition by Z-VAD-FMK was determined using the Caspase-Glo 3/7 Assay System (Promega). PC3 cells were plated at 1 × 104 cells per 96 well plate and 24 h later were either treated with docetaxel ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was assessed 3 days postinfection by MTS assay using a Cell Titer 96 aqueous cell proliferation kit (Promega) or Viral ToxGlo™ (ToxGlo assay) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated in triplicates with increasing doses of in-house small molecule inhibitor drugs and a DMSO vehicle control for 3 days and subsequently assessed for cell viability by measurement of ATP with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Signal intensity was read on a Glomax™ 96 Microplate Luminometer (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... and 656 bp of murine Mef2d 3’UTR (pMIR-Mef2d) were subcloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega). Correspondent seed-sequence mutated Dual-pMIR-report plasmids were obtained using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used ...
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bioRxiv - Microbiology 2020Quote: ... the target cells were resuspended at 3×105/mL in prewarmed culture medium that contains EnduRen live cell substrate (Promega) at a final concentration of 17 ng/ml and incubated for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
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bioRxiv - Microbiology 2022Quote: Templates for in vitro transcription of gene-specific 300-500 nt dsRNA and DENV2 EMSA probes were generated by PCR introducing a T7 promoter sequence or a universal tag at both 5’ and 3’ ends using the GoTaq Flexi DNA Polymerase (Promega). If present ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Developmental Biology 2022Quote: ... The erc1b probe was made by cloning 1041 nucleotides of 3’ UTR from cDNA into the pGEM-T Easy Vector (Promega) using primers listed in Supplementary Table 1.
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bioRxiv - Neuroscience 2021Quote: ... Luciferase constructs were obtained by PCR amplifying the 3’UTR regions from cDNA extracts and cloning them into a modified phRL-CMV vector (Promega), as previously described (Favereaux et al. ...
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bioRxiv - Microbiology 2021Quote: ... primer TTC CGC AAG TTC ACC TAC C and the reverse (3’ – 5’) primer CGG GCC GGC CAT GCT TTA CG with GoTaq Flexi DNA polymerase (Promega) and the following cycling conditions ...
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bioRxiv - Neuroscience 2021Quote: ... Visualization of the amplification products was done with ethidium bromide on a 3% electrophoresis gel and sizes were assessed (Bench Top 100bp DNA Ladder; Promega). Negative controls were performed with H2O or by omitting the reverse transcription phase.
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Neuroscience 2022Quote: ... the plasmid gene expression under the regulation of both Nogo-A 3’UTRs was evaluated by measuring firefly and Renilla luciferase activities using the Dual-GLO luciferase assay system (Promega) in an Infinite M200 plate reader (Tecan ...
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bioRxiv - Systems Biology 2022Quote: ... and 72h in 3-5 wells for each condition (mean values of 3-5 technical replicates are provided for each donor) using a multiwell plate reader Glomax (Promega). Each 2h incubation was performed at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... was added to a final concentration of 3 mM followed by the addition of 5 µl of T7 Enzyme Mix and 50 U RNasin RNase Inhibitor (Promega). To produce transcripts for mock transfections the GTP concentration was increased to 7.5 mM and cap analogues were not added ...
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bioRxiv - Cancer Biology 2022Quote: ... the 3’UTR of FOXP2 was amplified from FOXP2 3’UTR construct and ligated into a pmirGlo Dual-luciferase miRNA Target Expression Vector (Promega) to form 3’UTR-luciferase reporter vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Pro-apoptotic caspase 3/7 activation was measured in worms harvested from mice following drug treatment using the Caspase-Glo 3/7 Assay Kit (Promega). Worms were harvested from either the mesenteries or liver of mice ...
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bioRxiv - Molecular Biology 2019Quote: Validation of hsa-miR-548ba and hsa-miR-7973 direct binding to target gene 3’UTR was performed by using Dual-Glo Luciferase assay system (Promega) according to the user manual ...
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bioRxiv - Cell Biology 2020Quote: Measurements of caspase activities in cells were performed using the commercially available Caspase-Glo 3/7 Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of tRNAPhe(GAA) was performed using a 5’ biotinylated complementary oligonucleotide (5’-biotin-TGGTGCCGAAACCCGGGATTGAACCGGGG-3’) coupled to Streptavidin Magnesphere Paramagnetic particles (Promega). Annealing of specific tRNA was performed in 1 x TMA buffer (Tris-HCl pH 7.5 10 mM ...
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bioRxiv - Pathology 2020Quote: Reporter vectors were generated by inserting the Fam210a 3’UTR fragments containing wild-type or mutated seed sequence into the miRNA reporter vector pmirGlo (Promega). All the primers used to generate the reporter vector were listed in supplemental information ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were incubated for 3 days at 35°C with 5% CO2 and cell viability was evaluated using the CellTiter Glo (Promega) post the 3-day incubation ...
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bioRxiv - Cancer Biology 2021Quote: The full length 3’ UTR of c-Myc was cloned into the pmirGLO Dual-Luciferase miRNA target expression vector (Promega). Luciferase activity was assayed in transfected cells using the Dual-Luciferase Reporter Assay (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... PAT-PCR was performed using a 3’ UTR-specific forward primer and a y300 PAT universal C10 primer with GoTaq Green Master Mix (Promega). The primer sequences are provided in Table S1 ...
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bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Trpc4 and Trpc5 mRNAs was performed on 3 µl of cDNA from single cells in a 30 µl reaction volume containing 1X GoTaq Flexi buffer (Promega), 2 mM MgCl2 ...
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bioRxiv - Immunology 2019Quote: HEK cells were seeded at 3.0 × 106 cells in 10 cm dishes and transfected with 3 μg of the indicated DNA construct using FuGENE HD (Promega). 24 h post-transfection ...
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bioRxiv - Genetics 2021Quote: ... cell viability followed by caspase 3/7 activity were measured using CellTiter-Fluor™ Cell Viability Assay kit (Promega, G6080) and Caspase-Glo® 3/7 Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... n=4 vaginal) and rUTI (n=3 urine) cohorts belonging to B2 phylogenetic group was extracted (Wizard Genomic DNA Purification Kit, PROMEGA) and sequenced by Illumina NovaSeq 2×150 nt ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNAs were amplified (see Supplemental Table 3 for primer sequences) and ligated into pGEM-T vector (Promega, Madison, WI). To generate RNA substrates for EMSA ...
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bioRxiv - Cell Biology 2019Quote: ... 5’ GA CTC GAG GTT GCA CCT AGT GGA T 3’ The PCR product was first cloned into pGEM-T easy vector (Promega), verified by sequencing and subsequently cloned into pGEX5.1 vector using the EcoRI and XhoI sites ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Microbiology 2020Quote: ... glacialis A3 by filtering cells onto a 3-μm polycarbonate filter and using the Wizard SV Genomic DNA Purification kit (Promega) following the manufacturer’s instructions and quantified on a Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin chymotrypsin or alpha lytic protease (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays require washing the harvested cells 3 times in PBS prior to lysis in passive lysis buffer (Promega, E1941). NLuc activity was assayed using the Nano Luciferase Assay Kit (Promega ...
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bioRxiv - Immunology 2021Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and two of the aliquots were digested separately overnight using chymotrypsin (Mass Spectrometry Grade, Promega) or alpha lytic protease (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Immunology 2022Quote: ... that introduced a 5’ KpnI site and a 3’ XhoI site and cloned into the pGL3 firefly luciferase vector (Promega). Site directed mutagenesis was performed as described above ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of RNA from fractions corresponding to polysomes (3 fractions after the 80S monosome peak) were pooled and digested with RQ1 DNase (Promega) and cleaned-up using RNA Clean&Concentrator-5 kit (Zymo Research ...