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Citations for Promega :
3851 - 3900 of 4685 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CytoTox-OneTM homogeneous membrane integrity assay kit and Caspase-Glo® 3/7 assay system kit were from Promega, WI ...
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bioRxiv - Genomics 2023Quote: ... at 0.5 uM or DMSO (vehicle) followed by measurement of Caspase activity via the Caspase-Glo 3/7 assay (Promega). Results are representative of at least 3 independent experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Cancer Biology 2024Quote: ... A similar assay format was used to measure apoptosis with the Caspase-Glo® 3/7 3D Assay (Promega). Cells were seeded and treated as described above for 72 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 0.5 μg of “driver” plasmid + 0.5 μg of SVA “reporter” plasmid (pAD26) using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were co-transfected with 0.5 μg of “driver” plasmid (pTMO2F3) + 0.5 μg of pAluneoTet using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of plasmid was transfected using Fugene HD (Promega, Madison WI, #E2311). Plasmid DNA was mixed with 8 µL Fugene and 100 µL media and incubated at room temperature for 15 minutes before being added to cells ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... HALO-MultiDsk (2 mg) was incubated with 200 µl of HaloLink resin (Promega) in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... 12.50 μl of 2× PCR Green master mix (GoTaq DNA polymerase; Promega, USA) and 8.50 μl nuclease free water ...
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bioRxiv - Biochemistry 2020Quote: ... Seven other trypsins were used and are denoted as Trypsin-2 (V5111; Promega), Trypsin-3 (786-254 ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of 2× SYBR Green PCR Master Mix (Promega, Madison, WI, USA) and sterile milliQ water to a final volume of 15 μl per tube ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total RNA were treated with RQ1 RNase-Free DNase (Promega) and processed with the ImProm-II Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...
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bioRxiv - Immunology 2022Quote: ... Samples were again diluted to 2 M urea and digested with trypsin (Promega) (1/100 ...
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bioRxiv - Microbiology 2019Quote: ... cells were treated with MEK1/2 specific inhibitor U0126 (20 μM final; Promega). Transfections with siRNAs were performed using JetPRIME (Polyplus ...
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bioRxiv - Physiology 2020Quote: ... PCR amplicons were resolved on a 2% agarose gel (Promega Corporation, Madison, USA) pre-stained with Ethidium bromide (0.5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... and RFP (to 2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). After 24 h cells were treated with DMSO or VbP (10 µM ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μl of isolated genomic DNA was used for the genotyping PCR (Promega) using respective protocols.
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bioRxiv - Molecular Biology 2022Quote: ... and 2 micrograms from each sample were DNAse I treated (Promega, Madison, WI) following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of extracted RNA was treated with RQ1 RNase-Free DNAse (Promega), then used for cDNA synthesis using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified byPCR using SYBR® Green PCR Master Mix and ABI Prism7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 µL of 500 ng/µL Trypsin Platinum (Promega, Madison, WI, USA). Thereafter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Enzymatic digestion was performed by adding 2 μg trypsin (Gold, Promega, Madison USA) in TEABC 100 mM and incubating overnight at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in 50 μl TdT buffer containing 2 mM rGTP (Promega, E603B) and 20 U TdT at 37°C for 15 min for rGTP tailing41 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Genetics 2021Quote: ... cell supernatant from untransfected and cells transfected with IL2 constructs was diluted 1:12 with complete media and used to perform the IL2 Bioassay (Promega, JA2201). Luminescence (RLU ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA, FC-121-1030; .25uL 1% digitonin, Promega G9441; 1xPBS;) gently shaking (300rpm on Eppendorf thermomixer ...
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bioRxiv - Molecular Biology 2020Quote: ... DF-1 cells were transfected with RCASBP-(A)-β2m-sgRNA or MOCK RCASBP-(A)-EGFP-sgRNA using ViaFectTM Transfection Reagent (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified PCR-amplified promoter fragments (∼1 kb) were cloned between KpnI and XhoI sites in firefly luciferase reporter vector pGL3-Basic (Promega, USA). The resulting plasmids were named as SHR-Hmgcr and WKY-Hmgcr which contain −985 bp to +20 bp region of SHR and WKY Hmgcr promoter sequence ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesised using 1 µg of total RNA in a 20 µL final volume using the GoScript Reverse Transcription System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... 20×103 cells were seeded in a 24-well plate and co-transfected using PEI with pGL3-Chaserr-reporter vector (100 ng) and psiCHECK-1 (100 ng, Promega, C8011). Firefly and Renilla expression were measured using Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: Around 1×105 cells were treated as indicated prior to lysis and RNA isolation using the ReliaPrep™ RNA Miniprep kit (Promega). Approximately 1μg of total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... senders were stained at a density of 1.00E+07 cells/mL on ice for five minutes in the presence of either 1 µM HaloTag-Alexa Fluor 488 (Promega G1001) or 3.5 µM HaloTag-Alexa Fluor 660 (Promega G8471) ...
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bioRxiv - Biochemistry 2021Quote: ... WT or ADAR1-KO HEK293T cells in 12-well plates were transfected with 400 ng ml-1 of firefly luciferase under control of the IFN-β promoter (pIFN-Luc, Promega), 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK ...
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bioRxiv - Plant Biology 2020Quote: ... using 1 µg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then alkylated using 15mM chloroacetamide at 37°C for 30min and further digested using sequencing-grade modified trypsin (1/25, w/w, trypsin/protein; Promega, USA) at 37°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein pellet was resuspended in 190 ul of 50 mM ammonium bicarbonate and digested with 20 ul of 1 ug/ul trypsin gold (Promega V5280) at 37°C for 4 hours.
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...