Labshake search
Citations for Promega :
3901 - 3950 of 4181 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
-
bioRxiv - Microbiology 2023Quote: ... The copy numbers of the control DNA/RNA were calculated based on the molecular weight and absorbance at 260 nm of RNA that had been serially diluted (≥3 steps) and/or fluorescence using the Quantus Fluorometer (Promega, Madison, WI, USA) (n = 3).
-
bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
-
Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2024Quote: The Luciferase reporter vector were constructed via insertion of uc.110 conserved region and 3’UTR of MFRP downstream of Renilla luciferase stop codon in psi-CHECK2 dual luciferase vectors (Promega, Madison, WI, USA). The insertions were validated by sequencing ...
-
bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
-
bioRxiv - Bioengineering 2021Quote: ... Samples were diluted 10X in 50 mM ammonium bicarbonate after which 4 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) was added before overnight incubation at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... infected target cells were washed with DPBS and the medium was replaced with 50 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells continued to be scanned every 8 h for a further 4 days before being assessed for the number of viable cells using Cell Titer Blue® (Promega) according to the manufacturers instructions ...
-
bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
-
bioRxiv - Immunology 2021Quote: The PCR product of the targeted region within exon 4 of the TRDC gene was subcloned into the pGEM-T Easy Vector system (Promega, Germany) in accordance with the manufacturer’s protocol and then transformed into XL10 bacteria ...
-
bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... digoxigenin-labeled probes were synthesized from the full-length medaka cDNA of oct-4 (stem cell marker also known as pou5f1; [38]) using pGEM®-T Vector (Promega) linearized plasmid ...
-
bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
-
bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
-
bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
-
bioRxiv - Microbiology 2023Quote: ... infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
-
bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
-
bioRxiv - Microbiology 2024Quote: The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- tetrazolium (MTS) assay was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... the protein pellets were resuspended in 100 µL 0.6M urea in 100mM TEAB buffer and 4 µg trypsin (Promega, Cat#V511B) was added for digestion at 1:25 enzyme:protein at 500 rpm at 37°C overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... Levels of extracellular ATP were measured every 10 mins for 4 hours by using a luciferase activity assay kit (RealTime-Glo™ Extracellular ATP Assay, Promega) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were collected 4- and 6-hours post-transfection (hpt) for Luciferase measurement using Dual-Luciferase® Reporter Assay System (Promega) and RNA extraction for RT-qPCR assay using Luna® Universal One-Step RT-qPCR (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... Urea was reduced to 1 M by addition of 500 μl AB and overnight digestion was performed at 37 °C with 5 μl of freshly prepared 0.2 μg/μl trypsin (Promega) in AB ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were then diluted to 2 M urea final concentration via the addition of 20 mM HEPES pH 8.0 and digested overnight with 5 µg/ml sequence-modified trypsin (Promega) at room temperature.
-
bioRxiv - Microbiology 2020Quote: ... venezuelae macrocolonies that were grown for 30 h at 30°C on MYM agar were pooled and resuspended in 200 µl ice-cold stop solution (5% phenol (pH 4.3) in 98% ethanol) and RNA was isolated using the SV Total RNA Isolation Kit (Promega). After elution ...
-
bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
-
bioRxiv - Immunology 2021Quote: ... for 48h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by 5 min quenching with 100 mM glycine and permeabilization with 0.5% Triton X-100 (Promega, Madison, WI) in 1 × PBS for 15 min ...
-
bioRxiv - Microbiology 2020Quote: ... Urea was reduced to 1 M by addition of 500 μl AB and overnight digestion was performed at 37 °C with 5 μl of freshly prepared 0.2 μg/μl trypsin (Promega) in AB ...
-
bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
-
bioRxiv - Microbiology 2021Quote: ... Urea was reduced to 1 M by addition of 500 ml AB and overnight digestion was performed at 37°C with 5 ml of freshly prepared 0.2 mg/ml trypsin (Promega) in AB ...
-
bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 72hr at 37°C with 5% CO2 and then measured by CellTiter-Glo assay (Promega). Briefly ...
-
bioRxiv - Biophysics 2021Quote: ... The reaction was incubated at 30 °C for 5 hours and 2.5 µL of the reaction mix was added to 50 µL of luciferase assay reagent (Promega) pre-equilibrated in room temperature ...
-
The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... a second jar of xylene for 5 min then incubated in a series of ice-cold solutions with 0.0025% RNasin Plus (Promega): 100% ethanol for 1 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Liquid was removed and gel pieces were dehydrated in 100% ACN at RT for 5 min at 2,000 rpm followed by rehydration in 100 mM NH4HCO3 containing 14 ng/μL trypsin (Promega) on ice for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
-
bioRxiv - Microbiology 2021Quote: ... Ten nanograms of template DNA was added to 5 μl Green GoTaq Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 1.25 units of GoTaq DNA Polymerase (Promega ...
-
bioRxiv - Pathology 2022Quote: ... G490 5 × All-In-One RT MasterMix (abm) was used for reverse transcription according to the manufacturer’s protocol (Promega). The qRT-PCR analysis was performed in 96-well plates using the BIO-RAD CFX96 detection system ...
-
bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
-
bioRxiv - Plant Biology 2020Quote: ... proteins were precipitated overnight with 5 volumes of cold 0.1 M ammonium acetate in 100% methanol and digested with sequencing-grade trypsin (Promega) and each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (40Ul/µl stock, 5 ml/ml of buffer, Promega). We rinsed sections ...
-
bioRxiv - Microbiology 2021Quote: ... seriolae gDNA was extracted from 5-day old cultures using the Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK cells were transfected with 5’UTR-reporters for 24h using ViaFect reagent according to the manufacturer’s protocol (Promega). Next ...
-
bioRxiv - Immunology 2021Quote: ... Luminescence was measured after 20 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).
-
bioRxiv - Immunology 2021Quote: ... Luminescence was measured after 20 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).