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Citations for Promega :
3701 - 3750 of 4181 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 1 to 2 µg of RNA was used for DNase treatment as per the protocol provided by the manufacturer (Promega, Madison, Wisconsin, USA). This was followed by cDNA synthesis using reverse transcriptase kits from Applied Biosystems (High capacity cDNA synthesis kit ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded into 24-well plate and cultured to 80% confluence when they were co-transfected with 4 μg of either TOP flash or FOP flash vector and 1 μg of pRL-TK (Renilla-TK-luciferase vector, Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen) ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
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bioRxiv - Pathology 2021Quote: ... benthamiana leaves were collected at 48 h after infiltration and then incubated with 1 mM luciferin substrate (catalog no. N1110; Promega Biotech, Beijing, China). LUC images were captured with a Tanon-5200 Multi Chemiluminescent Imaging System (Tanon ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellets were reconstituted and digested with endoproteinase Lys-C (Alpha Laboratories, UK) for 1 hour at room temperature and trypsin (Promega, Madison, WI, USA) overnight at 35°C.
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bioRxiv - Biochemistry 2022Quote: ... transfer plasmid was co-transfected with GAG/POL and VSVG plasmids into HEK293T or 293FT cells using FuGENE HD (Promega, 3.33:1 FuGENE:DNA) and the medium was replaced 6–8 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... on the day before transfection and were transfected with 1 μg of plasmid DNA per well using FuGENE-HD reagent (Promega, product number E2312) per manufacturer guidelines ...
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bioRxiv - Developmental Biology 2022Quote: Samples for CaTS-RNAseq were collected in the same way as for CaTS-ATAC but all buffers were supplemented with 1 µL/ml of RNasin Plus (Promega, Cat. No. N2615). After fixation and quenching ...
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bioRxiv - Immunology 2020Quote: ... washed beads were then resuspended in 35 μL of ammonium bicarb buffer to which 1 μL sequencing grade porcine trypsin was added (Promega, Cat. No. V5111). Immune complexes were digested on bead for 1 hr at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The samples after pull-down assay and co-immunoprecipitation were also digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight in the same manner ...
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bioRxiv - Microbiology 2022Quote: ... the aqueous phase was added to 1 volume of 70% ethanol and loaded on ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s recommended protocol for oligo dT-primed synthesis ...
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bioRxiv - Pathology 2020Quote: ... The partial sequence encoding the nucleoprotein from HPWMoV was amplified using specific primers (Table 1) from two-fold diluted cDNA with GoTaq® Flexi DNA polymerase (Promega, WI, USA) with the following reaction conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... and digested with trypsin at an enzyme-to-substrate ratio of 1:50 (wt/wt, modified sequencing grade, Promega, Madison, Wisconsin. United States) for 16 hours at 30°C ...
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bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded on poly-lysine coated glass coverslips at a concentration of 1 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) two days before measurement.
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bioRxiv - Microbiology 2021Quote: ... The protein solution was further diluted down to less than 2 M urea with 50 mM Tris-Cl (pH 8) and incubated with 2 μg of modified trypsin (w/w, 1∶50) (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Tryptic samples were subsequently diluted to 2M urea in 100mM Tris/HCl pH 8 and digested over night at 37°C (Promega, enzyme:protein 1:100). Samples were adjusted to pH 2 using 10% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2021Quote: Isolated protein aggregate fraction was solubilized for 1 h at room temperature with the use of 10 µl 0.1 % ProteaseMAX™ surfactant (Promega, cat. no. V2072) in 50 mM ammonium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s protocol for oligo dT-primed synthesis ...
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bioRxiv - Genomics 2021Quote: ... We added tagmentation buffer (1x TD buffer, 0.015 µL TTE Mix V50 [Vazyme, #TD501], 0.625x protease inhibitor cocktail [Promega, #G6521], and 1 mM MgCl2) to reach a volume of 10 µL per well and then incubated the plates at 55 °C for 1 h ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: The cytotoxicity of all compounds (except ARINA-1 that interferes with the assay) was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Cat. # G1780), which measures LDH enzyme release ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-methionine-labeled Ctb or its derivatives were produced in coupled in vitro transcription and translation reaction (IVTT, at 30 °C for 1 h) using TNT Quick Coupled Transcription/Translation System (cat#L1170, Promega, Madison, WI, USA). Prey proteins were diluted in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Developmental Biology 2024Quote: ... buffer (containing 10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 1% BSA, 0.1% Tween, 1mM DTT, 1U/ul RNaseIn from Promega, 0.1% NP40, and 0.01% Digitonin) and incubated on ice for 4 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... was amplified by PCR using tnnc1a-CDS primers (Table 1) and cloned into the pGEM®-T Easy Vector Systems (Promega, catalog #: A1360). A tnnc1a cDNA clone was confirmed by sequencing and used to make digoxigenin-labeled riboprobes with the Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2024Quote: ... cells was transfected with 250ng of each of 2 pX330 constructs (containing Cas9 and 1 sgRNA) and 500 ng of the plasmid template with FuGene HD transfection reagent (Promega Corporation, Madison, WI) in the recommended conditions ...
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bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Neuroscience 2024Quote: ... For experiments cells were plated onto poly-D-lysine coated glass coverslips supplemented with 1 μM all-trans retinal and transfected using FuGENE® HD (Promega, Madison, USA) the next day ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G, US], 0.2 U/µl RNAsin Plus RNase inhibitor [Promega, N2615, US]).
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bioRxiv - Molecular Biology 2024Quote: ... was added and samples were digested for two hours at 37°C followed by addition of 1 μg of sequencing-grade Trypsin (Promega, Ref. No. V5113) and digestion overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed 3 times for 5 minutes in TBST or TBST-XL for mucin-2 and incubated with anti-mouse IgG HRP Conjugate (1:10000, Promega, Cat no. W4021) or anti-rabbit IgG HRP Conjugate (1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed overnight in a ThermoMixer at 2000 rpm at 37 °C with a protein-to enzyme ratio of 1 to 100 for both LysC (Wako, Osaka, Japan) and Trypsin (Promega, Madison, WI, USA). Tryptic peptides were acidified with 1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2024Quote: ... On-pellet in-solution protein digestion was performed in 100 µL 50 mM ABC (pH 8) by adding Trypsin/LysC (Promega, 1:50 ratio) to precipitated ASC proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:50 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072).
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and after 5 h the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega), according to manufacturer’s instructions ...