Labshake search
Citations for Promega :
301 - 350 of 5481 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: Caspase activity was measured using a chemiluminescent assay kit (Promega Caspase-Glo® 3/7) in 96-well format using an Analyst HT plate reader (Molecular Devices ...
-
bioRxiv - Cell Biology 2020Quote: ... Caspase activation was measured using the CaspaseGlo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions for the indicated time point presented in the figure legend ...
-
bioRxiv - Cell Biology 2023Quote: ... Apoptosis activities were detected using substrate from the Caspase-3 Glo Apoptosis kit (Promega, WI).
-
bioRxiv - Developmental Biology 2019Quote: ... In vitro transcription and translation reactions were performed using a TNT T7 coupled reticulocyte lysate system as recommended by the supplier (Promega, Charbonnieres-les-Bains, France). 1 µG of the plasmid DNA template was transcribed and the protein was translated at 30°C for 90 min.
-
bioRxiv - Neuroscience 2022Quote: ... the cDNA fragments corresponding to the coding region or 3′-UTR of mouse Inka2 cDNA were subcloned into a pGEM-3Z plasmid vector (Promega) for in vitro transcription ...
-
bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... Predicted binding sites were cloned and inserted into the pmirGLO vector (Promega, Madison, USA). For reporter assays ...
-
bioRxiv - Biochemistry 2020Quote: ... binding was performed between the soluble fraction (supernatant) and glutathione-conjugated magnetic beads (Promega) pre-equilibrated with lysis buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
-
bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
-
bioRxiv - Cell Biology 2021Quote: ... Caspase activation was measured using the Caspase Glo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... apoptosis was quantified using the Apo-ONE homogeneous caspase-3/7 assay kit (Promega, Madison, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... Cytotoxicity was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay kit (Promega) by determining Formazan absorbance at 590 nm on a Tecan Safire automated plate reader ...
-
bioRxiv - Microbiology 2022Quote: Caspase 3/7 activity was measured using the Caspase-Glo assay kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity and cell viability were determined by ApoTox-Glo assay kit (G6321, Promega). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... This was followed by a second round of TBST washes before incubation with HRP conjugated goat anti-mouse Ab (1:5000 Ab in 3 % BSA in TBST; Promega Corp.) for an hour ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
-
bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
-
bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
-
bioRxiv - Biophysics 2021Quote: Some protein kinase assays were performed using the ADP-Glo™ assay kit (Promega), which measures the generation of ADP in a kinase reaction ...
-
bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... the binding solution contains 1.0 µl of NanoLuc substrate stock solution (Promega, Madison, WI, USA). After addition to one well ...
-
bioRxiv - Physiology 2023Quote: ... The DNA binding activity of NF-κB was assayed according to the protocol from Promega Corp ...
-
bioRxiv - Biochemistry 2024Quote: ... an apo-TDO2 binding molecule (structure not disclosed) was coupled to the NanoBRET 590SE (Promega) dye to create a fluorescent tracer ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
-
bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
-
bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were subjected to Caspase 3/7 activity measurement with Caspase-Glo assay kit (Promega, Madison USA). Briefly ...
-
bioRxiv - Immunology 2019Quote: Cell viability and caspase 3/7 activity were measured using Non-Radioactive Cell Proliferation Assay kit (Promega) and Caspase-Glo® 3/7 assay kit (Promega) ...
-
bioRxiv - Neuroscience 2019Quote: Genomic DNA extracted from 3 ml fresh EDTA-blood (wizardR Genomic DNA purification Kit, Promega, Madison, WI) was used for genotyping ...
-
bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
-
bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were either treated with 50ng/ml rat Nerve Growth Factor (NG; Promega, Madison, WI, USA) at 0 ...
-
bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
-
bioRxiv - Cancer Biology 2019Quote: ... Marinnisen and the pG5 Luc (minimum promoter containing 5 sites for GAL4 binding) was purchased from Promega. The expression plasmid for Nrf2 WT ...
-
bioRxiv - Molecular Biology 2020Quote: ... FLuc levels were measured 3-days post-AAV administration using a Luciferase 1000 Assay System kit (Promega Cat#E4550) per manufacturer’s instructions and read on a Veritas luminometer with settings ...
-
bioRxiv - Cancer Biology 2022Quote: ... Caspase-3 and -7 activity was measured by Caspase-Glo 3/7 Assay (Promega) according the manufacturer’s instructions using a BioTek Synergy H1 plate reader.
-
bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega) and caspase activity was normalized to cell number by performing the CellTiter-Glo Luminescent Cell Viability Assay on the duplicate plate.
-
bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega), and caspase activity was normalized to cell number by performing the CellTiter Glo Luminescent Cell Viability Assay on the duplicate plate ...
-
bioRxiv - Cancer Biology 2022Quote: ... caspase-3 and -7 activities were measured by Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... 3 μg of purified RNA was incubated with 3 units of RQ1 DNase (Promega) at 37°C for 30 mins ...