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Citations for Promega :
201 - 250 of 5481 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the oligo with NF-κB consensus binding element (Promega) was end-labeled by T4 polynucleotide kinase (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Cancer Biology 2021Quote: The activity of caspase-3/7 was measured by Caspase-Glo-3/7 assay kit according to the manufacturer’s instructions (Promega). Cell death was assessed by an Annexin-V FITC binding assay (Miltenyi ...
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bioRxiv - Biochemistry 2021Quote: Caspase-3/7 activity was quantified by fluorometric assay using ApoONE Homogeneous Caspase-3/7 Assay Kit (Promega, US). AU565 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptosis was determined using Caspase 3/7-Glo assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Translation of the DNA library was performed using T7 RiboMAX™ Express Large-Scale RNA Production System (Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were added alone or in combination at the indicated concentrations (0.25-4 pmol) to in vitro translation reactions (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified constructs were used as inputs for reactions (final reaction concentration of 6.96 ng/µL) using the TnT Quick Coupled Transcription/Translation System (Promega). Reactions were performed as described for the TnT system ...
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Biophysics 2022Quote: ... Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) was used to cleave MBP-Q44-HttEx1 at 22 °C (Boatz et al. ...
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity was measured using a luminescence Caspase Glo 3/7 assay kit (G8090; Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... caspase-3/7 activities in cells were measured using a luminometric assay kit Caspase-Glo 3/7 (Promega, Madison, WI). The amount of luminescence was measured on the Victor3™ multilabel reader (PerkinElmer Inc.).
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bioRxiv - Molecular Biology 2019Quote: ... samples corresponding to 4.44×105 cell equivalents of the translation reaction were put on ice and mixed with 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...
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bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
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bioRxiv - Cell Biology 2021Quote: ... and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system, Promega) and were incubated with the bead bound c-MET for 4h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual-Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control WT replicon ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL in vitro translation reactions were performed in the linear range using 2.8 nM mRNA in the Flexi RRL System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Developmental Biology 2020Quote: ... SrpB (Waltzer et al 2002) and Med1A (Immarigeon et al 2019) by in vitro transcription/translation coupled reactions using rabbit reticulocyte extracts (TnT-Promega) isoforms labeled ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins used for EMSA experiments were synthesized in-vitro from pTNT plasmids using TNT T7 Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control wt replicon ...
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bioRxiv - Microbiology 2022Quote: ... CERTL destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N- terminal myc epitope tag ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Developmental Biology 2022Quote: VC-Ubx and VC-Scr were cloned in the PcDNA3 vector and produced with the TNT-T7-coupled in vitro transcription/translation system (Promega) for EMSAs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or RepNDK-RNA was incubated at 37 °C for 12 h in a translation system and FluoroTect GreenLys tRNA (Promega), with neither CTP nor CDP to preclude RNA replication ...
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bioRxiv - Cell Biology 2023Quote: ... For in vitro import assay WT and MLS-TFEB cDNA were cloned into the pGEM-4 vector and transcribed in vitro using the TNT Quick coupled translation system (L1170, Promega), by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pSP64 in vitro translation vector and translated with a 35S radiolabelled methionine label using the TNT coupled reticulocyte lysate system (Promega) following manufacturers guidelines ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5µg was analyzed using the Caspase 3/7 glo kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular apoptosis was analyzed with Caspase-Glo 3/7 assay kits (Promega), which measures Caspase-3/7 activity ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Promega Caspase-Glo™ 3/7 Assay Kit was obtained from Promega Corporation (Madison ...
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bioRxiv - Physiology 2023Quote: ... and the Caspase-Glo 3/7 Assay Kit was purchased from Promega.
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bioRxiv - Neuroscience 2024Quote: ... and the Caspase-Glo 3/7 assay kit (#G8090, Promega, Nacka, Sweden) were used in 96-well plates (Sarstedt #82.1581.001 ...
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bioRxiv - Molecular Biology 2023Quote: ... ATRX 5’-TGAAACTTCATTTTCAACCAAATGCTC-3’ and 5’-ATCAAGGGGATGGCAGCAG-3’ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... murine epidermal growth factor (20 ng/ml; Promega, Madison, WI), and heparin (50 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...