Labshake search
Citations for Promega :
3351 - 3400 of 3490 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
-
bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
-
bioRxiv - Systems Biology 2019Quote: ... 100 μg of proteins were precipitated by methanol-chloroform method and digested overnight at 37°C with 1 μg trypsin/Lys-C mixture (Promega Cat#V5073) 19 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and thiol groups were alkylated with 50 mM iodoacetamide for 1 h at room temperature in darkness) and digested in situ with sequencing grade trypsin (Promega, Madison, WI), as described (Shevchenko et al. ...
-
bioRxiv - Cell Biology 2019Quote: ... the rein was equilibrated in IP buffer with 1 mM DTT and incubated overnight at 4°C with 5 μL ProTEV Plus (Promega, Sunnyvale, CA) in 100 μL total reaction volume ...
-
bioRxiv - Neuroscience 2021Quote: H4 cells were transfected with equal amounts of SynT or WT aSyn together with synphilin-1 using FuGENE6 Transfection Reagent (Promega, Madison, USA) in a ratio of 1∶3 according to the manufacturer’s instructions (Lázaro et al ...
-
bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cellular viability/toxicity of the THP-1 derived macrophage system was established with the MTS cellular proliferation-viability assay (Promega, Cat#G3582).
-
bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat. #A6141) addition of 1 μg trypsin/lysC mix (Promega, Cat. # V5071) at 37 °C for 16 hours ...
-
bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
-
bioRxiv - Cell Biology 2021Quote: ... Briefly 70-90% confluent cells in 6-well plated were transfected with 1 µg CRISPR/Cas9 plasmids per well by using FuGENE 6 (Promega, Madison, Wisconsin). After 24 h post-transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
-
bioRxiv - Biophysics 2021Quote: ... DNA constructs of full-length wt and T654A EGFR (1 μg) were transiently transfected into CHO-K1 cells using FuGENE HD Transfection Reagent (Promega, Madison, WI). For single-molecule measurements ...
-
bioRxiv - Microbiology 2022Quote: ... The PCRs were performed in a final volume of 20 μl of amplification mix containing 1 U of GoTaq polymerase (Promega, Madison, WI), 1× GoTaq buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
-
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... the -0.6 kb promoter of zebrafish pu.1 gene and the -1.1 kb promoter of zebrafish zbtb14 gene were cloned into the PGL3 basic vector (Promega, Madison, WI, USA). Tol2-plasmid was constructed by insertion of pu.1 DN under mpeg1.1 promoter (2 kb) ...
-
bioRxiv - Microbiology 2019Quote: ... The next day they were transfected over 24 hours with a DNA-Fugene HD mixture at a ratio of 1 μg DNA to 3 μl Fugene (Promega, Southampton, UK) according to the manufacturer’s instructions (Western analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... the SCN cultures were maintained in a 35-mm petri dish with 1 mL culture medium containing 0.3 mM D-luciferin (Promega, Madison, WI, USA) at 36 °C ...
-
bioRxiv - Microbiology 2019Quote: ... the wells were washed and 200 µl of a 1:5,000 dilution of secondary Anti-Mouse IgG antibody conjugated to HRP (Promega Corporation, Madison, WI) in PBS + 2% BSA was added to each well ...
-
bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
-
bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega, catnr: V5111) at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: To determine Caspase 3/7 activity in THP-1 SAMHD1 KO and CTRL cells the Caspase-Glo 3/7 assay (Promega, Walldorf, Germany) was used according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
-
bioRxiv - Microbiology 2019Quote: ... The membrane was then washed with TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse IgG (diluted 1:1,000 in TBS-T) (Promega, Madison, WI, USA) for 1 h at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... Total DNA of 500ng of ODInCas9 plasmid together with ZFN-AAVS1 plasmid at a ratio of 1:10 was mixed and transfected by lipid-based transfection using FuGene HD (Promega, Madison, WI) according to manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
-
bioRxiv - Immunology 2020Quote: ... −75 to −1 in relative to the transcription start site (TSS) of Il13 gene) and P150 (−150 to −1) were cloned into pGL3-basic luciferase vector (Promega, Madison, WI) in BglII and HindIII cloning sites ...
-
bioRxiv - Developmental Biology 2021Quote: ... in media containing 1% FBS and tested for expression of luciferase using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega #E7120). Luciferase expression was measured using a Flexstation 3 plate reader (Molecular Devices) ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA was ligated with a 5’-hydroxylated ‘Processed Start Site’ (PSS) RNA adaptor oligomers (onkh031) by RNA Ligase 1 (Promega or NEB) and then purified to remove excess oligomers (NEB Monarch RNA Cleanup kit) ...
-
bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
-
bioRxiv - Molecular Biology 2022Quote: ... Primer sets for real-time PCR (Table 1) were used to amplify target regions from genomic DNA and cDNA as templates by the GoTaq DNA polymerase (Promega, WI, USA). For real-time PCR experiments ...
-
bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
-
bioRxiv - Physiology 2024Quote: ... target cDNA fragments were amplified with the primers listed in Table 1 and ligated into pGEM T-Easy vector (Promega, Madison, WI). The cDNA fragments were PCR amplified with M13 primers and purified with the FastGene Gel/PCR Extraction Kit (Nippon Genetics ...
-
Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... The gradients were centrifuged in an SW41 rotor at 200,000xg for 1hr Fractions were collected from air-gradient interfaces and complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
-
bioRxiv - Developmental Biology 2024Quote: ... using a spinning disk confocal microscope (see in vivo confocal imaging paragraph above) after embedding the embryos in 1% low-melting agarose (Promega, Cat# V2111) in PBS 1X in a glass bottom 60μ- Dish (35mm high ...
-
bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
-
bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: ... measurements 1×105 T cells were cultured in white 96 well plate and analysed using NAD/NADH-Glo™ luminescent assay (Promega, G9071), according to the manufacturer instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
-
bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
-
bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples containing 5-10 µg of protein were mixed with 0.25-0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...