Labshake search
Citations for Promega :
2951 - 3000 of 3490 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
-
bioRxiv - Molecular Biology 2021Quote: ... cells were co-transfected with 2 µg of pWH464 (pSpCas9(BB)−2A-GFP (pX458)) expressing the corresponding targeting sgRNA and 1 µg of the corresponding donor oligo or plasmid using the Fugene6 (Promega). To generate miR-182Δ and miR-183Δ mESCs ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL RRV-P3 plasmid or 3 μL of cDNA from rose tissue or nuclease-free water (Promega, Madison, WI). The final volume of the LAMP reaction was 25 μL.
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were digested with 0.5 µg of lysyl endopeptidase (Wako) at room temperature for 4 hours and were further digested overnight with 1 µg trypsin (Promega) at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from cells at the exponential phase (OD600 ~1) with the acidic hot phenol method 41 and treated with DNase (Promega). 50 μg of total cellular RNA was incubated with 1 μM of the corresponding DNAzyme ((Supplementary Table S2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
-
bioRxiv - Cell Biology 2022Quote: ... coated with carbon and 0.1% gelatin as described previously (Maeda et al., 2020) and treated with 20 nM HaloTag SaraFluor 650T ligand (GCKA308; Promega) for 15 min before fixation ...
-
bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
-
bioRxiv - Molecular Biology 2021Quote: ... Liberibacter OmpA was amplified using SgfI and PmeI restriction sites (Table 1) and was digested using Flexi Enzyme Blend (Promega). Similarly ...
-
bioRxiv - Molecular Biology 2021Quote: ... The GAL1 promoter was amplified from yeast genomic DNA using primers 4588-1/XH5103 and ligated into pGEM-Teasy (Promega) to create pCB4554 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
-
bioRxiv - Systems Biology 2020Quote: ... to a final concentration of 1% DOC before overnight digestion at 37 °C with lysyl endopeptidase (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) at an enzyme-substrate ratio of 1:100 for both ...
-
bioRxiv - Microbiology 2019Quote: ... Total RNA concentration was quantified using a Nanodrop and 1 μg of total RNA was treated with RQ1 DNase (Promega) at 37°C for 45 mins ...
-
bioRxiv - Genomics 2020Quote: ... Supernatant was removed and pellet was resuspended in 400 μl of sort buffer (1 mM EDTA 0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... SDC concentration was adjusted to 1% and 120 µg of proteins from each bacterial culture were digested by addition of trypsin (Promega) in a 1:50 (enzyme:protein ...
-
bioRxiv - Bioengineering 2019Quote: ... Cultures were grown for 16 h as described above and then 1 mL of culture was used to isolate DNA using the Wizard DNA Purification Kit (Promega). A Nanodrop 2000 (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... with a 20 mm Microwell and covered with a mixture of 1% Low Melting Point Agarose (prepared with Ringer’s Solution; Promega, V2111) and thin albumin in order to immobilize the embryo on the dish.
-
bioRxiv - Systems Biology 2019Quote: ... The sample was digested for 16 h at 37C using a 1:50 (w/w) ratio of Sequencing Grade Modified Trypsin (Promega):protein in the gel ...
-
bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
-
bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 200 µl of digestion buffer and incubated for 5 h with 1 µg of trypsin (Promega) at 37°C ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using 1% agarose gel electrophoresis and QuantiFluor dsDNA System in a Quantus fluorometer (Promega). NextRAD libraries were constructed by SNPsaurus (SNPsaurus ...
-
bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... transfected cells growing in 12-well plates were carefully washed twice with warm phosphate-buffered saline (PBS) followed by treatment with 120 µl 1× Passive Lysis Buffer (PLB, Promega) by shaking for 10-15 min at room temperature ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 6228 and Uniport ID: P62266) and its N-terminally truncated mutant (deleted residues 1-41) were cloned into pEGFP-N1 vector (Promega) between KpnI and XhoI sites as described in Ref.19 ...
-
bioRxiv - Microbiology 2019Quote: ... Purified CsrA1/2 diluted in CsrA-binding buffer (1 µl in 10mM Tris-HCl, 10mM MgCl2, 100mM KCl, 10mM DTT, 10% glycerol, and 10 U RNasin [Promega]) was added ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA quality and quantity were assessed using 1% agarose electrophoresis and the QuantiFluor dsDNA System with a Quantus fluorometer (Promega). Nextera-tagmented reductively-amplified DNA (nextRAD ...
-
bioRxiv - Molecular Biology 2019Quote: ... qPCR amplifications (12 μl) were done in duplicates using 1 μl of cDNA and the GoTaq Probe 2X Master Mix (Promega) on a LightCycler 480 (Roche) ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
-
bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized via reverse-transcription of 1 μg of RNA using M-MLV Reverse Transcriptase (Promega (Wisconsin, USA). cDNA was used to analyse differences in gene expression by qRT-PCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
-
bioRxiv - Immunology 2021Quote: ... JEG-3 viability after 1 h salubrinal pretreatment followed by 48 h ZIKV infection was measured by CellTiter-Glo Assay (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were then washed three times with 1 × PBS and blocked with 3% Blot-Qualified bovine serum albumin (BSA, Promega) in 1 × PBS containing 0.05% Tween-20 (TPBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Twenty microliters of HPLC-grade water were added to reduce urea concentration and trypsin digestion was performed using a 1:20 (enzyme:protein) ratio of sequencing grade modified trypsin (Promega, V5111) and 18 h incubation at 37 °C on a ThermoMixer (Eppendorf ...
-
bioRxiv - Biochemistry 2020Quote: ... CDK2-cyclin A-SKP1-SKP2 (full-length SKP1 and full-length wild-type SKP2 and S64A mutant) and CDK2-cyclin A-p27KIP1M (residues 1-106) were assayed using the ADP-GloTM assay (Promega) using a p107 peptide (peptide SPIK+RXL ...
-
bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The proteins were diluted 1:5 with 100 mM ammonium bicarbonate and digested overnight at 37°C following the addition of 1 µg of Trypsin/Lys-C protease mixture (Promega) and calcium chloride to a concentration of 1 mM ...
-
bioRxiv - Cancer Biology 2020Quote: ... Collagenase IV (all at a concentration of 1 mg/ml) and Dispase (2mg/ml) in the presence of RNase inhibitors (RNasin (Promega) and RNase OUT (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with the ImProm-II Reverse Transcription System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... ShhN dsDNA coding for residues 1-198 of murine Shh was obtained by PCR using Pfu polymerase (Promega cat # M7741) and the following primers ...
-
bioRxiv - Cell Biology 2021Quote: ... Firefly and renilla luciferase activities were measured using an Optocomp 1 luminometer and a Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
-
bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
-
bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
-
bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
-
bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...