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Citations for Promega :
2801 - 2850 of 3490 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and the sample was digested at 37 °C with sequencing-grade trypsin (Promega; 1:50 protease:protein w/w ratio) or at 25°C with Glu-C (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For Halo-tag labeling cells were allowed to re-attach for 1 hour under hypoxic conditions and then incubated with 0.5 uM of Janelia Fluor 549 (Promega) for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Cell Biology 2021Quote: cDNA synthesis was performed using 1-5 μg of total RNA with random hexamers and reverse transcriptase (Promega, GoScript). Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then diluted with water (1:5) and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and peptides separated into 3 fractions on a 200 µL StageTips packed with three Empore™ SPE Disks SDB-RPS (3M ...
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bioRxiv - Cell Biology 2021Quote: ... then samples were digested overnight at 37°C on a rotator with modified trypsin (1:20 trypsin:sample ratio) (Promega). Peptides were desalted using SepPak C18 columns (Waters ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 1 μg/mL freshly prepared coelenterazine h (Promega). Cells were plated in white-walled ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 0.1M Triethylammonium bicarbonate (TEAB) and overnight (∼16 h) digestion was performed with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C with constant mixing using a thermomixer ...
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bioRxiv - Bioengineering 2022Quote: ... various concentrations of the compounds were mixed with 1 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... plates were washed 5 times with wash buffer (PBS with 1% v/v bovine serum albumin (Capricorn Scientific) and 0.05% v/v Tween-20 (Promega)) ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:1000 dilution and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary IgG (W401B, Promega) was used in a 1:20,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with 0.1M ammonium bicarbonate solution to a final concentration of 1% sodium deoxycholate before digestion with trypsin (Promega) at 37°C overnight (protein to trypsin ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 minutes in the dark, RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, overnight at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... washed thrice with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibody (Promega, Madison, WI, USA). After four additional washes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Synthesis of cDNA was performed with 1 μg of total RNA by using the GoScriptTM Reverse Transcription System (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Cell Biology 2019Quote: ... Either HCT116 cells or DLD-1 cells were transfected with guide plasmids and donor plasmid using ViaFectTM (#E4981, Promega) on 3.5cm dishes ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Biophysics 2019Quote: hTERT RPE-1 cells stably expressing Smo-Halo were transfected with OMP25-SNAP using FuGENE HD Transfection Reagent (Promega) one day before imaging ...
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bioRxiv - Biophysics 2019Quote: ... hTERT RPE-1 cells stably expressing Smo-Halo were transfected with Sec61β-SNAP using FuGENE HD Transfection Reagent (Promega) one day before imaging ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and the cells were lysed with 30 µl/well of 1× cell lysis buffer (Promega) for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... the protein sample was digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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bioRxiv - Biophysics 2019Quote: ... the fluid chambers were exposed to a PBS pH 7.4 solution containing 1% glutaraldehyde to subsequently immobilize the reference beads (Spherotec) and the HaloTag ligand (Promega).
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bioRxiv - Developmental Biology 2020Quote: ... Tryptic digests were performed overnight after addition of 46 μl of 100 mM Hepes pH 7.6 and 4 μl of 1 μg/μl Trypsin Gold (Promega).
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bioRxiv - Immunology 2020Quote: ... The HIV-1 infectivity was evaluated by the luminescence intensity following Steady-Glo® luciferase assay (Promega; WI, USA), following company specified methodology ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Cell Biology 2019Quote: ... for 20 min in the dark at room temperature and digested using 1 μg trypsin (sequencing grade; #V5111, Promega) per 100 μg of protein overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... at room temperature (r.t.) for 4 hours and then further digested overnight with 1:50 (w/w) trypsin (Promega) at r.t ...
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bioRxiv - Immunology 2020Quote: ... Samples were then diluted with 50 mM HEPBS pH 8.3 to reduce urea concentration down to 1 M and digested with trypsin (Promega) at 1:10 enzyme:substrate ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... MI) and digested overnight in 25 mM ammonium bicarbonate (ambic) with 1:200 (w/w) dilution of trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Cell Biology 2020Quote: ... then diluted to a final concentration of 1 M prior to in-solution digestion with 0.2 µg/µl trypsin (Sequencing grade (Promega) overnight at 37°C ...