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Citations for Promega :
2751 - 2800 of 4516 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... ~80mg of tissue is first placed in 1.5 mL microcentrifuge tubes containing 250 μL of 1x passive lysis buffer (Promega) and homogenized ...
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bioRxiv - Immunology 2021Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was discarded and the pellet was resuspended in a small volume of SSC cocktail (3xSSC [Sigma; S0902], 0.04% BSA, 40mM DTT [Sigma; 43816], 0.5U/mL RNasin plus [Promega; N2615]) to reach a concentration of approximately 2000 cells/µL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell culture medium was replaced by pH indicator-free culture medium containing 0.32 mg/ml MTS (Promega, Mannheim, Germany) and 0.0073 mg/ml phenazine methosulfate (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: 5 million THP-1 expressing HA-Ubiquin (from pTRIP-SFFV-Blast-2A-HA-Ubiquitin) were plated in 5ml of fresh medium containing 5µg/ml digitonin (Promega) per well in a 6 well plate the day of the stimulation ...
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bioRxiv - Synthetic Biology 2022Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL) (herring sperm DNA, Promega) were added onto the cell pellet ...
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bioRxiv - Microbiology 2024Quote: ... grown overnight in 50 ml LB with ampicillin and the resulting mutant plasmid pool was purified by midiprep (Promega). The mutant plasmid pool was transformed to BY4742 using the high-efficiency LiOAC/PEG method (Gietz and Schiestl 2007) ...
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bioRxiv - Molecular Biology 2023Quote: ... fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL, herring sperm DNA, Promega), was added onto the cell pellet ...
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bioRxiv - Bioengineering 2023Quote: ... imaging was performed 10 min after intraperitoneal (i.p.) injection with 200 μL of 15 mg/mL luciferin substrate (Promega). The total flux of luminescence was calculated by gating a region of interest (ROI ...
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bioRxiv - Neuroscience 2023Quote: ... after which the slice pieces were fixed in 4% PFA in PBS (with 0.1 U/ml RNase inhibitor, Promega) for 15 mins and dissociated into single cells through careful trituration ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−N-loop-PE+N-loop- AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−peptide-PE+peptide-AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and seeded in 96-well plates at a density of 3.5×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). Following overnight incubation ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then divided in two to obtain tryptic and Glu-C peptides and diluted to final concentration of 0.1 mg/mL with 50 mM NH4HCO3 for Trypsin digestion and with PBS for Glu-C (Promega) digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Immunology 2020Quote: Caspase-1 and Caspase 3/7 activities were measured using Caspase-Glo® 1 and Caspase-Glo® 3/7 assay (Promega) respectively ...
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bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Microbiology 2020Quote: ... 0.3% xylose and 1% low melting temperature agarose (Promega) on slides ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of RNAse inhibitor (40 U/µl, Promega) was added to stop the reaction ...
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bioRxiv - Molecular Biology 2019Quote: Goat anti-mouse IgG HRP 1:2000 (Promega W4021)
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:1000) and b-actin-peroxidase (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Biochemistry 2020Quote: ... 1x reaction buffer (from Promega (containing 1 mM Co2+) for Supplementary Fig ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... HRP-conjugated anti-mouse (Promega, catalog W4021, 1:6000), HRP-conjugated anti-goat (Abcam ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... HRP-conjugated anti-rabbit (Promega, catalog W4011, 1:6000), HRP-conjugated anti-mouse (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... and 1 unit T4 ligase with associated buffer (Promega). The following thermocycler program was run ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µg of sequencing-grade modified trypsin (Promega, USA) was added to each solution ...
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bioRxiv - Neuroscience 2020Quote: ... and Donkey anti-rabbit HRP (Promega W401B, 1:10,000). Soluble protein samples were normalized to REVERT total protein stain (Li-Cor).
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bioRxiv - Neuroscience 2020Quote: ... US) and TubβIII (mouse, 1:1000, G712A, Promega, UK). Slides were then incubated with the appropriate fluorophore-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... The HRP-conjugated goat anti-mouse (1:10,000; Promega) and goat anti-rabbit IgG (1:10,000 ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin was added to 1:50 (m/m) (Promega) and incubated overnight at 37°C with 600 rpm shaking on a thermomixer ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Promega) was added to each RNAi well for induction of dsRNA synthesis and plates were incubated for 3-4 hours at 30°C and 155 rpm ...
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bioRxiv - Genomics 2022Quote: ... or anti-Mouse IgG HRP Conjugate (Promega, 1:2,000) diluted in blocking buffer followed by washing in TBST ...
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bioRxiv - Immunology 2022Quote: ... 16 % dimethylformamide) supplemented with 0.5 µl 1 % Digitonin (Promega) and 5 µl Tn5 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-rabbit IgG HRP (Promega, WB 1:10,000)
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bioRxiv - Biophysics 2022Quote: Cells were lysed in 1× passive lysis buffer (Promega) and combined with 4× Laemmli sample buffer (Bio-Rad ...