Labshake search
Citations for Promega :
2551 - 2600 of 4516 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
-
bioRxiv - Molecular Biology 2023Quote: ... Two μL of reverse transcription buffer 5X were added and the samples were incubated at 20°C for 15 min before addition of 1.5 μL of dNTP mix (1 mM of dATP, dGTP, dCTP, dUTP) and 1 μL of AMV enzyme (PROMEGA). Reverse transcription was performed at 42°C for 45 min and synthesized cDNA was recovered by ethanol precipitation as described above ...
-
bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Genomics 2022Quote: ... Nuclei were resuspended in 200 μL resuspension buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) and transferred to a 1.5 mL Eppendorf tube tube ...
-
bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
-
bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
-
bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
-
bioRxiv - Cell Biology 2024Quote: ... 125 ng of which was the reporter plasmid mixture (1:1 ratio of 8xGli-nanoluc/pGl3b to pGL4.53[luc2/PGK] (Promega)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were then diluted to 1 M urea and subjected to digestion with trypsin (Promega, 1:50 w/w) overnight at 37⁰C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 1 nM of linear template DNA) and supplemented with 1 µL of BODIPY-Lys-tRNALys (FluoroTect GreenLys, Promega) to incorporate fluorescently labelled lysine residues in the synthesized proteins ...
-
bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... swab tips were placed into DNA/DNase/RNase-free 1.5 ml Eppendorf tubes containing nuclease-free water (Promega). The swab shafts were cut with a pair of sterile dissection scissors to allow closing of the tubes ...
-
bioRxiv - Biochemistry 2019Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... converted 1µg of DNase-treated RNA to cDNA with 20nt Oligo-dT (25µg/mL; IDT) and rRNAsin (Promega). Precipitated cDNA was resuspended in water at 20ng/µL of original RNA concentration ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were digested by addition of 0.25 μg of sequencing grade trypsin dissolved in 50 mM acetic acid at 0.5 μg/mL (Promega) overnight at 37 °C with vigorous shaking ...
-
bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... aliquots of the enzyme were removed from the freezer and were treated with thrombin (0.1 kU/mL, Promega) overnight at 4 °C in enzyme storage buffer (20 mM HEPES ...
-
bioRxiv - Cancer Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lysis buffer (0.1% v/v NP-40, 0.1% v/v Tween-20, 0.1mg/mL Digitonin ((Promega, Southampton, UK) in resuspension buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... DMEM media was aspirated from the cells and 500 µL of hypotonic lysis buffer (50 mM Tris.HCl pH 7.4, 25 units/mL DNase I (Promega), 0.2 % (v/v ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 200 μL of the cell/supernatant suspension was treated overnight with 80 μg/ml proteinase K (Promega) in 1x proteinase K digestion buffer (10 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
-
bioRxiv - Biophysics 2019Quote: ... the beads were incubated in a PBS solution containing 25 μg/mL of the HaloTag ligand O4 (Promega) for at least 4 h at constant rotation (room temperature) ...
-
bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2μg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... In-gel digestion was performed by adding 25 μL of 4 μg/mL sequencing grade modified trypsin (Promega) in 25 mM ammonium bicarbonate ...
-
bioRxiv - Cell Biology 2022Quote: ... then alkylated with 1.9 mg/mL chloroacetic acid before proteolytic digestion with 0.2 μg sequencing grade ArgC (Promega) and incubation at 37°C for 16 h ...
-
bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
-
bioRxiv - Immunology 2022Quote: ... The samples were then subjected to in-gel trypsin digest (Trypsin Gold, Mass Spectrometry Grade. Promega. 20μg/ml). Samples were loaded in an UltiMate 3000 HPLC autosampler system (Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL salt-tris solution buffer supplemented with 0.2 U/ul RNasin Plus RNase Inhibitor (Promega). The tissue material was spin down at 500 x g for 5 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: Mid-log phase fusobacterial cells (OD600 of ∼ 0.5) were harvested by centrifugation and combined in equal volumes (0.1 ml) with the BacTiter-Glo Reagent (Promega) in multi-well plates (Cellstar) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
-
bioRxiv - Microbiology 2024Quote: ... and then incubated for an additional hour at 55°C after adding 20 mg/ml proteinase K (Promega). DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research) ...
-
bioRxiv - Immunology 2024Quote: ... and cells were treated with 3µg/ml LPS O111:B4 and 0.25% (vol/vol) Fugene HD Plus (Promega) to cause transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 1 μg LysC (Wako) overnight at room temperature and then with 1 μg sequencing grade trypsin (Promega) for 6 hours at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
-
bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
-
bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
-
bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... was dispensed into white 96 well luminometer plates and 45 µL of 1 x NanoGlo Lysis Buffer containing 1:1000 NanoGlo substrate (Promega) was injected into wells ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells for gene targeting were transfected with 500 ng of donor and gRNA plasmids in ratio 1:1 using ViaFect (Promega) transfection reagent according to the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
-
bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...