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Citations for Promega :
2451 - 2500 of 5641 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... generated by cloning the entire coding sequence of mouse APOBEC1 (mAPOBEC1) amplified from mouse cDNA by PCR into pGEM-T-easy (Promega), and cloning it into the EcoRI and SalI sites of pEGFP-C2 (Clontech) ...
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bioRxiv - Plant Biology 2023Quote: The HLB1 coding sequence corresponding to the N-terminal 200 amino acids was amplified by PCR using the following primers and was cloned into pGEM-T-easy vector (Promega). The NdeI and XhoI fragment of HLB11-200 was cloned into pET28a vector (Novagen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Microbiology 2023Quote: ... Shigella conjugants that grew at 30°C and were Cb60 resistant were screened by PCR for the presence of lpxE and the large virulence plasmid using GoTaq (Promega) and primer sets lpxE-F/lpxE-R (lpxE ...
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bioRxiv - Microbiology 2023Quote: ... The kan gene was then inserted between the upstream and downstream 05515-05525 flanking PCR fragments and simultaneously inserted into the pGEM-7Zf vector (Promega) in the BamHI and XhoI sites using the In-Fusion kit according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... MSIL4-GFP/MSIL4G-tagged version used for complementation and RIP corresponds to the fusion of a genomic PCR product containing MSIL4 promoter (primers TL3527(HindIII)-TL3528(SalI)) fused with a second PCR cDNA fragment (primers TL3529(SalI)-TL3530(BamHI)) cloned initially in pGEM T Easy (Promega). After sequencing the fusion DNA has been cloned in the binary vector CTL579 containing GFP cDNA sequence ...
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bioRxiv - Biochemistry 2023Quote: ... Northern probes were generated from gel-purified PCR products corresponding to the RNAi inserts and radioactively labelled using the Prime-a-Gene labelling system (Promega).
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bioRxiv - Biochemistry 2022Quote: ... The 400 base pair region surrounding the sgRNA target was then amplified by PCR (forward primer: 5’-CCCAGAGAGGAGGCTGTAGA-3’; reverse primer: 5’-AAAGGCCTCCCAGGGGTTAT-3’) with GoTaq DNA Polymerase (Promega). The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Developmental Biology 2023Quote: The highly conserved 998-bp core sequence of Xenopus tropicalis MFCS1 was amplified by PCR with specific primers (Table S3) and inserted into the Acc65I and XhoI sites of the pGL4.23 vector (Promega, E8411). Then the minimal promoter of the pGL4.23 vector was replaced with a 486-bp sequence of Xenopus laevis shh promoter (Fig ...
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bioRxiv - Bioengineering 2023Quote: ... Presumptive screening of 10 colonies per mPilA variant was performed via colony-PCR using GoTaq® Green Master Mix (Promega) and forward and reverse primers Diag:71301:MPilA:All:FWD and Diag:71301:MPilA:All:REV (SI Table S1) ...
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bioRxiv - Neuroscience 2023Quote: ... of neuropeptide and neurotransmitter marker genes were PCR-amplified from zebrafish brain cDNA or genomic DNA and cloned into the pGEM-T Easy vector (Promega). Three glutamate decarboxylase genes (gad1a ...
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bioRxiv - Plant Biology 2023Quote: ... Genotyping PCRs were performed using Promega Hotstart Green MasterMix following the recommended conditions from the manufacturer (Promega, Madison, WI, U.S.A.).
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Sequences encompassing the SD site and the env gene were acquired after cloning the PCR fragments inserted into pGEM-Teasy (Promega). Sanger sequencing with primers env7254(+ ...
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bioRxiv - Microbiology 2023Quote: ... gondii glyceraldehyde 3-phosphate dehydrogenase 2 (GAPDH2, ML5049/ML5680) or TgAPT1 (ML4097/ML4098) were used for subsequent PCR amplification with GoTaq polymerase (Promega) for twenty-five cycles as follows ...
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bioRxiv - Plant Biology 2023Quote: The cDNA of BdNRT2A was first amplified by PCR using specific forward primer 70F2 and reverse primer 70R2 before being cloned into the pGEMT-easy vector (Promega). Clones with correct sequence were used to produced appropriate PCR product for Gateway cloning ...
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bioRxiv - Microbiology 2023Quote: The region upstream of the genes of interest was amplified by PCR with proper primers (Table S4) and cloned into the pGEM-T easy plasmid (Promega). The insert was removed by digestion and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the lacZ gene ...
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bioRxiv - Cell Biology 2023Quote: The Human SART1 mutant resistant to siSART1#3 and its deletions mutants (amplified by PCR) were subcloned into pCI-neo Mammalian Expression Vector (Promega).
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bioRxiv - Systems Biology 2023Quote: ... and the bands at the predicted product size were excised and purified with a gel and PCR clean-up system (Promega). Sanger sequencing was completed by the Micromon facility at Monash University ...
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bioRxiv - Plant Biology 2024Quote: ... Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega). Sequencing was performed at LGC Genomics (Berlin ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Cancer Biology 2024Quote: Human H1-0 promoter sequence (nucleotides −351 to +161 from TSS) was PCR-amplified from REH cell genomic DNA and inserted into Firefly luciferase vector pGL4.22 (Promega, #E6771) at KpnI and HindIII restriction sites ...
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bioRxiv - Biochemistry 2024Quote: ... subtilis genomic DNA containing RsbT/RsbU was amplified by PCR using GoTaq DNA polymerase mix for thirty cycles without modification from the manufacturers protocol (Promega). DNA sequencing revealed that inserts had on average one to two mutations per product ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were then cloned into the BamHI restriction site of luciferase T7 control DNA plasmid (Cat. No. L4821, Promega). Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA library is pre-amplified in a first PCR (6 cycles) followed by size selection with ProNex beads (Promega) to remove short cDNAs and primer dimers ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified through an 0.8% agarose gel, recovered (ZymoResearch, USA) and ligated into pGEM-T (Promega, USA). The ligation reaction was transformed into DH5α Escherichia coli (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Cell Biology 2024Quote: ... The monoclonal cells were screened for successful incorporation in the genomic locus of the fluorescent tag by PCR using GoTaq DNA Polymerase (Promega). The genome-edited cells were confirmed through imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA was extracted from a small ear biopsy and PCR was performed with GoTaq® Hot Start Polymerase (Promega) using specific primers (Supplementary Table 1).
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bioRxiv - Developmental Biology 2024Quote: ... The selected marker genes were amplified by polymerase chain reaction (PCR) from the cDNA using GoTaq G2 Hot Start Green Master Mix (Promega), and primers designed using the PodMur_1.0 reference genome.88 Primers were designed to amplify 800-1200 bp of the gene of interest with approximately 500 bps in the 3’UTR and T7 and SP6 promoters attached to the reverse and forward primers ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleofected cells were incubated for 48 h prior to clonal amplification and screening for homozygous clones using target-specific PCR (GoTaq Hot Start, Promega) on genomic DNA using the following primers ...
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bioRxiv - Plant Biology 2020Quote: ... carried out using the RQ1-DNase kit (Promega), and were reverse transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... using the Wizard Genomic DNA Purification Kit (Promega). Cytosine methylation on target loci was assessed by Methylation-Sensitive Restriction Enzyme qPCR (MSRE-qPCR ...
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bioRxiv - Microbiology 2020Quote: Wizard Promega DNA extraction kits (Promega, Madison, WI) was used to extract DNA from overnight broth culture ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... using the GoTaq qPCR Master Mix kit (Promega). Gene expression was normalized to the housekeeping gene Rplp0 and expressed as fold change compared to CD11cWT samples ...
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bioRxiv - Genomics 2020Quote: ... with the Wizard Genomic DNA Purification Kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The RealTime-Glo Annexin V Apoptosis kit (Promega) was used as instructed to quantify the degree of apoptosis in HCASMC ...
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bioRxiv - Neuroscience 2021Quote: ... purified with Wizard Genomic DNA Purification kit (Promega) and confirmed via sequencing (GeneWiz) ...
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bioRxiv - Evolutionary Biology 2019Quote: The Wizard® Genomic DNA Purification kit (Promega) was used to isolated genomic DNA from overnight cultures ...
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bioRxiv - Microbiology 2019Quote: ... using the LEV DNA kit (AS1290, Promega, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Dual-Glo® Luciferase Assay System Kit (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by cDNA synthesis (Promega and Fermentas kits) and SYBR Green (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... were determined via Quantifluor dsDNA assay kit (Promega), modified 1,9-dimethylmethylene blue (DMMB ...
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bioRxiv - Neuroscience 2020Quote: An commercially available absorbance-based LDH kit (Promega) was utilized to measure the release of LDH to culture medium as an indicator of necrosis ...
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bioRxiv - Neuroscience 2021Quote: ... using the T7 or SP6 Riboprobe kit (Promega). 20 μm sections from fixed tissue were labeled and detected as previously described3 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated by GoScript kit (# TM316, Promega) using 4 μg total RNA with random primers ...
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bioRxiv - Microbiology 2020Quote: ... and 100μl of luciferase assay reagent (Promega Kit) added ...
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bioRxiv - Genomics 2021Quote: ... the Maxwell 16-cell DNA purification kit (Promega) or the MagNA Pure DNA isolation kit (Roche Molecular Systems ...