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Citations for Promega :
151 - 200 of 3098 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and translated using the nuclease-treated rabbit reticulocyte lysate (RRL; Promega). Typical reactions were comprised of 75% v/v RRL ...
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bioRxiv - Cell Biology 2021Quote: ... the mRNA was translated in a Flexi rabbit reticulocyte lysate (Promega) containing 35S methionine/cysteine (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were analyzed using a Dual-Luciferase Reporter Assay System (Promega) with a Tecan Microplate Reader (Tecan Infinite M1000) ...
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bioRxiv - Molecular Biology 2020Quote: ... which was synthesized in vitro with reticulocyte lysate systems (Promega, L5020), for 2 h at room temperature in binding buffer (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... and the lysate was treated with 20U/ml RNase A (Promega) and 60U/ml RQ1 DNase (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... The acquired mRNA was then translated in rabbit reticulocyte lysates (Promega) containing 35S- labelled Met and Cys ...
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bioRxiv - Immunology 2020Quote: ... Dual luciferase activity in the total cell lysates was quantified (Promega).
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were treated with RNAse free DNAse (Promega Corp) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA transcripts were then translated in rabbit reticulocyte lysate (RRL) (Promega) in the presence of L-35S-methionine (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro translated with a Rabbit Reticulocyte Lysate system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were then subjected to Dual-Luciferase Reporter assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl reactions contained 60% nuclease-treated rabbit reticulocyte lysate (Promega), 20 μM amino acid mixture minus leucine ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701, Promega) for 90 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA), and amplified using SYBR Green qRT-PCR SuperMix-UDG reagents (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 µl RT buffer 5x (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
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bioRxiv - Neuroscience 2021Quote: mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega), as previously described (23 ...
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bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
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bioRxiv - Biochemistry 2020Quote: ... Lep variants were then translated using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... reactions were prepared as per Rabbit Reticulocyte Lysate System (Promega Cat#L4960). 10 µL of reaction mixture was supplemented with 1 µg of either Renilla or Firefly luciferase mRNA alongside recombinant GST or GST-PKM1 ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) (Promega). Typical reactions were composed of 90% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μl of lysate was incubated with 50 μl luciferase reagent (Promega) and measured for 10 s using Lumat LB9507 (EG&G Berthold) ...
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bioRxiv - Microbiology 2022Quote: ... and a dual luciferase detection assay was conducted upon the lysates (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity in the lysates was analyzed using Luciferase Assay System (Promega) as per manufacturer’s instructions and as conducted previously (Vasudevan and Steitz ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μl of lysate was incubated with 50 μl luciferase reagent (Promega) and measured for 10 s using (Lumat LB9507 ...
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bioRxiv - Cell Biology 2023Quote: Lysates were digested from the beads with 100 ng of LysC (Promega) in 25mM HEPES pH 8.5 overnight at 37 °C with shaking at 1200 RPM in a thermomixer ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell lysates were harvested with 1X Passive Lysis Buffer (5X, Promega #E1941) diluted in water ...
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bioRxiv - Molecular Biology 2023Quote: ... and lysates were assayed for luciferase activity using Bright-Glo™ (Promega) on a 96-well using The VICTOR3™ Multilabel Plate Reader (PerkinElmer LAS Germany GmbH).
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bioRxiv - Microbiology 2024Quote: ... Lysates were then analyzed by the addition of luciferase reagent LAR (Promega) in white microtiter plates using a Tecan plate reader and represented as relative light units (RLU ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s protocol and reverse transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega) as follows ...
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bioRxiv - Microbiology 2022Quote: In vitro cytotoxicity of SMKIs on mock and/or virus infected A549 cells was determined using CellTiter-Glo 2.0 (CTG) Cell Viability Assay (Promega) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: Cytotoxicity of SMKIs on mock and/or virus-infected PCLS was determined using the LDH-Glo Cytotoxicity Assay (Promega) according to manufacturer’s protocols ...