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Citations for Promega :
101 - 150 of 3098 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Passive Lysis Buffer (Promega) and analyzed with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Reporter Lysis Buffer (Promega) and analyzed with the Luciferase Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclease-treated rabbit reticulocyte lysate translation reactions (Promega L4960) were set up in a 384-well plate (Thermo Scientific 164610 ...
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bioRxiv - Biochemistry 2021Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as previously described (Zong et al. ...
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bioRxiv - Cell Biology 2020Quote: ... NDUF13 precursors were synthesized using rabbit reticulocyte lysate (Promega) in the presence of [35S] methionine ...
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bioRxiv - Biochemistry 2021Quote: ... 17.5 µL of nuclease-treated rabbit reticulocyte lysate (Promega), 5 U RNase inhibitor (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Nuclease-treated rabbit reticulocyte lysate was from Promega (L4960), [35S] methionine (EasyTag EXPRESS 35S Protein Labelling Mix ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with Stop & Glo substrates (Promega) and luciferase activity was measured in a Synergy Neo2 reader (Biotek).
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bioRxiv - Microbiology 2020Quote: ... the TnT® Coupled Reticulocyte Lysate system (Promega; #L4610) was utilised as per the manufacturers’ instructions using 200ng of plasmid DNA ...
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bioRxiv - Immunology 2021Quote: ... All transcripts were translated in rabbit reticulocyte lysate (Promega) in the presence of L-35S-methionine (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each sample was added to Rabbit Reticulocyte Lysate (Promega) and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 47.5 µL of nuclease-treated rabbit-reticulocyte lysate (Promega), and one of four concentrations of HR1 peptide (0 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as described53,54 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and measured for luciferase activities using the Dual luciferase reporter assay system (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... transcripts were incubated with rabbit reticulocyte lysates (L4960; Promega) for 30 min at 37°C.
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bioRxiv - Biochemistry 2024Quote: ... Normalized cell lysate was first mixed with vivazine (Promega) to reach 1% vol/vol as recommended by Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Molecular Biology 2021Quote: 500 µl rabbit reticulocyte lysate obtained from Promega (L4151, untreated) was supplemented with 1 µl 12.5 mM Hemin (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... using the TnT Quick-Coupled reticulocyte lysate system (Promega #L1170) and 35S-Methionine (Perkin Elmer #NEG709A001MC) ...
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bioRxiv - Cell Biology 2019Quote: ... and lysates were analyzed using a GloMax® Luminometer (Promega). Transfection and expression efficiencies were normalized to the activity of β-galactosidase activities.
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Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entrybioRxiv - Biochemistry 2021Quote: ... The cell lysates were firstly digested with trypsin (Promega, USA) by the in-solution digestion method22 ...
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
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bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...
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bioRxiv - Molecular Biology 2022Quote: A rabbit reticulocyte lysate in vitro translation kit (Promega, L4960) was coupled to in vitro transcription with the supplementation of MgCl2 ...
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bioRxiv - Microbiology 2023Quote: Cell lysate was prepared by using passive lysis buffer (Promega). Firefly and renilla luciferase activities (Fluc and Rluc ...
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bioRxiv - Biochemistry 2023Quote: ... with the T7 TNT Coupled Reticulocyte Lysate System (Promega, L1170). Each substrate was amplified with primers by PCR (using template plasmids from the hORFeome collection or commercially available plasmids ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... Lysates were mixed with Firefly luciferase assay reagent (E1500 Promega) as per manufacturer’s protocol and scanned for luminescence in Tecan scanner.
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus -specific PCR products were cloned into pGEM-T Easy Vector (A1360, Promega Co., Madison, WI, USA) and used to transform E ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...