Labshake search
Citations for Promega :
1 - 50 of 3098 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
-
bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
-
bioRxiv - Molecular Biology 2022Quote: 1 ml nuclease-untreated Rabbit Reticulocyte Lysate (Promega L4151) was supplemented with 25 µM haemin ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
-
Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
-
Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
-
bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
-
bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
-
bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
-
bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Lysates for luciferase assay were prepared in 1× passive lysis buffer (Promega), 100 μl per well of a 24-well plate ...
-
bioRxiv - Cell Biology 2023Quote: Lysates for luciferase assay were prepared in 1× passive lysis buffer (Promega), 100 μl per well of a 24-well plate ...
-
bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
-
bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
-
bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
-
bioRxiv - Microbiology 2023Quote: ... Rabbit Reticulocyte Lysate (Promega), ATP regeneration system (0.5 mM ATP [Wako]) ...
-
bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
-
bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
-
bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
-
bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
-
bioRxiv - Biochemistry 2020Quote: Rabbit reticulocyte lysates (RRL, Promega) were used to investigate the inhibition of translation by DPR proteins ...
-
bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... synthesis was done by using 1 μg RNA as template and the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (Promega), in 20 μl reaction volume according to the instructions manual ...
-
bioRxiv - Biochemistry 2022Quote: ... In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System, Promega) and translated CFTR (domains ...
-
bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
-
bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of crude DNA lysate was used with 2x GoTaq Reaction Mix (Promega M7123) supplemented with 5% DMSO.
-
bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
-
bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
-
bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
-
bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
-
bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
-
bioRxiv - Biochemistry 2022Quote: ... or uncoupled reticulocyte lysate system (Promega). For the import reaction ...
-
bioRxiv - Cell Biology 2019Quote: ... and rabbit reticulocyte lysate system (Promega) respectively ...
-
bioRxiv - Biochemistry 2020Quote: The Rabbit Reticulocyte Lysate System (Promega) was used to determine the influence of internal modifications on translation efficiency of RNA F ...
-
bioRxiv - Biophysics 2023Quote: Rabbit reticulate lysate (RRL, L4960, Promega) is used as the artificial cytoplasm to fulfill the requirement of cytosolic factors necessary for nuclear transport (S ...
-
bioRxiv - Cancer Biology 2024Quote: Rabbit reticulocyte lysates (RRL) (Promega L4960) were treated with micrococcal nuclease (NEB M0247S ...