Labshake search
Citations for Promega :
1901 - 1921 of 1921 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The 25 µL polymerase chain reaction (PCR) mixture was the same for both mtDNA genes using 5x Green GoTaq® Flexi buffer (Promega), 0.2 mM dNTPs ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Microbiology 2024Quote: ... 2 μL of cDNA was added to the RT-PCR reaction mixture along with 10 μL of 2x GreenTaq polymerase (Promega, Madison), 1 μL of each MpFV-Hel forward and reverse primers (10 μM) ...
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bioRxiv - Cell Biology 2021Quote: ... Bait proteins were eluted with 25µl of elution buffer (2M Urea, 0.67M thiourea in 50mM Ammonium bicarbonate pH 8.0) containing LysC/Trypsin (Promega, Madison, WI, cat# V5071) to a final concentration of 5µg/ml followed by two elution with 50µl of elution buffer without LysC/Trypsin ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of supernatant was removed from all wells and replaced with 100 μl of Bright-Glo luciferase substrate reagent (Promega; catalog #E2610). After 2 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Correct protein size of 41.4 kDa for recEmTDH and 40.3 kDa for recMmTDH was confirmed by western blot using a mouse monoclonal anti-His tag antibody and an anti-mouse IgG (h+l) ap conjugate as secondary antibody (Promega, Dübendorf, Switzerland). Finally ...
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bioRxiv - Immunology 2024Quote: ... Then 100 μl of the co-culture was transferred to a white plate with an equal volume of Bright-Glo reagent (Promega, Madison, WI) and was incubated for 5 minutes before luminescence was measured.
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bioRxiv - Biochemistry 2022Quote: ... cells reached 50-60% confluence and were transfected with 350 ng of ADGRL3 + 350 ng 22F Glosensor reporter plasmid (E2301; Promega – a gift from R. Lefkowitz lab) + 9 ng of β2-adrenergic receptor + 2.8 uL of transfection reagent Fugene 6 ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from the OP and CL swab samples collected from chickens and tufted ducks using Maxwell® 16 Viral Total Nucleic Acid Purification Kit on a Maxwell® 16 System extraction robot (Promega, Madison, WI, USA). RNA was isolated from lung ...
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bioRxiv - Biochemistry 2024Quote: Kinase assay for GSK-3α and GSK-3β to assess the inhibitory potency of Psoralidin and Rosmarinic acid was performed employing GSK-3α and GSK-3β enzyme system (Promega Cat# V9361 and V9371, respectively) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined by removing 100 μL supernatant from all wells and adding 100 μL CellTiter-Glo reagent to the cells as described in the manufacturers protocol (CellTiter-Glo® Luminescent Cell Viability Assay, Promega, Madison, USA). After 2 minutes shaking on an orbital shaker ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... Three μL (6 ng on average) of template DNA was added to 5 μL 5x GoTaq Green Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 0.625 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of culture supernatant was removed from each well and replaced with 100 μL of ONE-Glo luciferase reagent (Promega, Madison, WI, Cat# E6110). The plates were gently mixed by rocking for 10 min at rt ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was quenched by adding 40 μl of 25 mM DTT and subsequently 5 μl of 100 mM CaCl2 and 25 µl of Trypsin stock was added (400 ng/μl Trypsin Platinum from Promega Corp. in 100 mM Tris-HCl [pH 8]) plus 115 μl of 25 mM NH4HCO3 (pH 8.5 ...