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Citations for Promega :
1851 - 1900 of 1921 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Luciferase activity of cell extracts was measured with Sirius L Tube Luminometer (Berthold) and the luciferase assay system (Promega).
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted with 120 μL of PBS before 1.2 μL of ProteaseMax surfactant (0.1 mg/mL in 100 mM ammonium bicarbonate, Promega, V2071) and sequencing grade trypsin (10 μL ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
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bioRxiv - Microbiology 2022Quote: Total RNA from each lung homogenate and nasal wash was isolated using the Maxwell RSC Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation), following the manufacturer’s suggested protocol ...
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bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were imaged using ChemiDocTM MP Imaging system using the Rhodamine filter and then counterstained with DiamondTM Nucleic Acid dye (Promega Corporation) to visualize the primer bands ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from leaf tissue using a modified protocol for Wizard(R) Magnetic 96 DNA Plant System from Promega (Madison, Wisconsin, USA). The copy number of integrated T-DNA in transgenic soybean plants was measured in duplex TaqMan assays ...
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bioRxiv - Biochemistry 2021Quote: ... After the capture step, beads were washed with PBS-casein and recovered using a Magnetic Rack (Ref Z5343, MagneSphere(R) (Promega, Madison, Wisconsin, USA), for tubes ...
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bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Maxwell 16 LEV simply RNA tissue kit in combination with a Maxwell nucleic acid extraction robot (Promega, WI, USA) for rabbit caecal content ...
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bioRxiv - Cell Biology 2024Quote: ... five µg protein were reduced by 2.5 mM dithiothreitol for 30 min at 37°C and alkylated with 10 mM iodoacetic acid for 30 min at 37°C in the dark before digestion by trypsin (Promega, Walldorf, Germany) in a protein to enzyme ratio 25:1 overnight at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Immunology 2020Quote: ... Total DNA from blood and urine (around 50 μL) was extracted using a Maxwell 16 automat with the Maxwell blood DNA and cell LEV DNA purification kits (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL of the medium at several time points was collected for gLuc expression assay using Renilla Luciferase Assay System (Promega) and a luminometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega).
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega). The ratio between Renilla and Firefly is calculated for each experimental condition.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed twice with 1X TBS-TT (10X TBS-TT is 10X TBS buffer with 5 mL/L Tween 20 and 5 mL/L Triton X) and once with 1X TBS before incubation with the secondary antibody (IgG anti-rabbit HRP conjugate, Promega) 1:1,000 diluted in blocking solution for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Microbiology 2024Quote: ... and the coding region of INKV L amplified by PCR and purified using the Wizard SV Gel and PCR Clean-up System (Promega). The INKV L fragment and the pINKV-L_linker backbone were digested with Aat II and Bsr GI (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of the reaction product was combined with 30 μL of a 1:50 dilution of Nano-Glo substrate (Promega) into a 364-well microplate ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNAs were extracted from the homogenized lung tissue samples (10% w/v) and nasal wash samples using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). The whole genomes of SARS-CoV-2 used in this research were amplified using a modified ARTIC protocol with several primers replaced or added (57 ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.