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Citations for Promega :
1901 - 1950 of 4085 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
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bioRxiv - Immunology 2022Quote: ... cells were washed 2 times with phosphate-buffered saline (PBS) and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega, Madison, WI). Using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: 3’ UTR fragments of miRNA target genes (for primer sequences see Table S2) were cloned into the XhoI/NotI sites downstream of the synthetic Renilla luciferase gene of psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 16.5 μg attB-plasmid library + 3 μg pCAG-NLS-Bxb1 + 2 μg pMAX-GFP + 2150 μL serum-free DMEM + 80 μL FugeneHD (Promega cat# E2311), then incubated at room temperature for 15 min and added dropwise to cells ...
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bioRxiv - Plant Biology 2024Quote: ... The Halo-DREB2D fusion protein was obtained by coupled in vitro transcription/translation for 2 h at 27 °C using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, WI, USA). Halo-DREB2D synthesis was verified by Western blot using Anti-HaloTag® Monoclonal Antibodies (Promega) ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were then diluted 4-fold with 100 mM ammonium bicarbonate to reduce urea concentration to 2 M and then added 5% (W/W) modified trypsin (Promega, Madison, WI). The pH was adjusted to 8.0 with 250 mM ammonium bicarbonate ...
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bioRxiv - Developmental Biology 2024Quote: ... tRFValCAC sequence was inserted into the multiple-cloning site at the 3’ end of the hRluc gene within the psiCHECK-2 Vector (Promega, cat # C8021). mESCs were transfected using the method described above ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, M1705, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 mM HEPES pH 7.4, 1mM MgCl2 with 1x c0mplete™ EDTA-free Protease Inhibitor Cocktail and 1U/μl RNasin® Plus RNase Inhibitor from Promega added right before the use) and incubating for 30 minutes on ice with pipetting every 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of repair template was transfected into 200,000 cells using a 3:1 ratio Fugene6:DNA (Promega), after overnight transfection cells were grown in fresh medium for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of pCMVi and 1 μg of pMDG (gifts from Greg Towers) using Fugene-HD (Promega, E2311). Virus was collected from 4x 10 cm plates 48 ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 mM NaCl, 1% NP-40, 0.5% SDS, 0.2 mM PMSF, 1 mM DTT, 10U RNase inhibitor from Promega, cOmplete Protease Inhibitor Cocktail from Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for 1 hour at room temperature with an HRP-conjugated secondary antibody (1:1000, Promega). Protein expression was revealed using the Clarity kit (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted 1:1 with TEAB buffer and digested by adding 0.1 µg of modified Trypsin (Promega) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega). The reaction was carried out at 42°C for 50 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega) in a total reaction volume of 25 μl ...
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bioRxiv - Genomics 2022Quote: ... After washing with TBST and incubation for 1 hour with anti-Rabbit IgG HRP Conjugate (Promega, 1:2,000) or anti-Mouse IgG HRP Conjugate (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... For immunostaining of the induced neurons following antibodies were used: anti-Tuj 1 (mouse; 1:2000, G7121, Promega), anti-MAP2 (chicken ...
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bioRxiv - Physiology 2022Quote: ... 1:1000 mouse anti-ATP1A1 followed by 1:2500 goat anti-mouse HRP-conjugated secondary (catalog #: W4021; Promega). p-ATP1A1Y260 and ATP1A1 bands were visualized with SuperSignal Pico utilizing the ChemiDoc.
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bioRxiv - Genetics 2020Quote: ... cells were washed with 1× phosphate-buffered saline (PBS) then lysed with 1× passive lysis buffer (E194A, Promega). Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:10,000 for WB) or goat-anti-rabbit IgG (W4011, 1:10,000 for WB) were purchased from Promega. APC anti-human CD63 (353008 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µL of extract was used as a DNA template for 1× Gotaq® Green Master Mix (Promega) 25 μL volume PCRs ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... cDNA was diluted at 1/80 and qPCR were performed using 1 μl of diluted cDNA (GoTaq Promega) and run on QuantStudio 3 Real Time PCR system ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL wash buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) ...
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bioRxiv - Molecular Biology 2023Quote: ... molar equivalents of the appropriate TF constructs were transfected into cells alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
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bioRxiv - Molecular Biology 2024Quote: ... an extra 1-hour room temperature incubation in 1:2500 anti-Mouse IgG-HRP secondary antibody (Promega, W4021) + 1% BSA + TBS was performed prior to Clarity Western ECL detection.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with 1:50 w/w LysC (Wako Chemicals) and 1:100 w/w trypsin (Promega) overnight at 37°C after dilution to a final urea concentration of 1M using 50mM Tris pH 8.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% Tween-20, 0.1% NP-40, 0.01% Digitonin, 1 mM DTT and 1 U/uL RnaseIn, Promega©) was added to cell pellets and incubated on ice for 3-5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... was added 1:1 to medium in triplicate wells and detected using a GloMax Luciferase Microplate Reader (Promega) with 0.5 second integration time ...
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bioRxiv - Cell Biology 2024Quote: ... HRP conjugated anti-rabbit (W401B; WB: 1:10,000) and anti-mouse (W402B; WB: 1:10,000) secondary antibodies were from Promega. Goat anti-Mouse IgG (H + L ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of random hexamer primers (Promega) and 1 μg of RNA in 16.125 μl ...
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bioRxiv - Cell Biology 2020Quote: ... Digested 1:50 v/v Trypsin (Promega) at 37°C o/n ...
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bioRxiv - Genetics 2021Quote: ... and α-βgal (1:100, Promega, Z3781). HRP-conjugated secondary antibodies in conjunction with the TSA system (Molecular Probes ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Halotag (Promega, G9281, 1:500), rabbit anti-Sox2 (Abcam ...
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bioRxiv - Genomics 2021Quote: 1% of lambda phage gDNA (D1221, Promega) was spiked-into 300ng gDNA to use as an unmethylated internal control ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 unit/µl RNasin Plus (Promega, N2615), 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL GoScript reverse transcriptase (A5003; Promega), and 7 μL RNase-free double-distilled H2O (N2511 ...
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bioRxiv - Microbiology 2020Quote: ... 1:1000 TuJ1 (monoclonal mouse, G712A, Promega); 1:250 NeuN (polyclonal chicken ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1% NLuc substrate (Nano-Glo, Promega) in the presence of release factors (4 nM eRF1 alone or with 4 nM eRF3a ...
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bioRxiv - Molecular Biology 2021Quote: ... trypsin-digested (1:75 w/w, Promega) and desalted (HLB μElution plate ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mg sequencing grade trypsin (Promega) per 200 mg total protein was added and incubated for 15 hours at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and α-βgal (1:100, Promega, Z3781). Embryo antibody staining was performed as described [24] ...
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bioRxiv - Plant Biology 2020Quote: ... 1 unit of RNase ONE™ (Promega) or 0.01 unit of RNase V1 (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µL of GreenLys solution (FluoroTect, Promega) was supplemented to a PUREfrex2.0 mix (total volume 10 µL ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
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bioRxiv - Systems Biology 2021Quote: ... 1 μL of 0.2 M DTT (Promega) was added ...