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Citations for Promega :
1701 - 1750 of 4085 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and 100 µL were seeded per well in 96-well plates at a density of 2×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). The following morning ...
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bioRxiv - Cell Biology 2023Quote: ... Intracellular fluorescence was monitored after 2 and 3 h of nanoalgosome incubation by spectrofluorimetric analysis using a microplate reader (Glomax, Promega). As vehicle-control ...
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bioRxiv - Microbiology 2024Quote: ... Tat and Rev and plasmid encoding spike of SARS-CoV-2 variants Delta or Omicron were transfected in HEK 293T cells using Fugene6 reagent (Cat. No. E2691, Promega) following manufacturer’s guidelines ...
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bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Neuroscience 2022Quote: The scATAC-seq peaks that overlapped obesity-associated SNPs were PCR amplified from human genomic DNA (see primers in Supplementary Table 2) and cloned into the pGL4.23 plasmid (Promega, E84111). The associated SNPs were then introduced into these plasmids by PCR amplification with primers containing the associated variants ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously [25] ...
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bioRxiv - Biochemistry 2022Quote: ... The RNA substrate was diluted in BLI buffer supplemented with a RNase inhibitor at a final concentration of 2 U/μl (RNasin Ribonuclease inhibitor (Promega)) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Physiology 2022Quote: ... Denatured proteins were then enzymatically digested into peptides upon incubation first with endoproteinase LysC (Wako Laboratories) at 25 °C shaking for 2 h and then with sequencing-grade trypsin (Promega) at 25 °C shaking overnight ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously20 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...
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bioRxiv - Physiology 2023Quote: ... The cultured media was replaced with 2 ml of DMEM media supplemented with 200 μM Beetle luciferin (Promega, Wisconsin, Madison). BMAL1Luc HDFs’ bioluminescence was measured continuously with a Kronos Dio Real-time luminometer (Atto ...
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bioRxiv - Immunology 2022Quote: ... was generated by cloning the regulatory region of Ikzf4 (2 kb upstream of the transcriptional start site) into the pGL3-Basic vector (Promega). EL4 T cells were nucleofected in combination with either a STAT5BCA ...
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bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Specific adapters 1 and 2 described in Table B were self-hybridized and then ligated to the ‘A’ tail ends by T4 ligase (Promega). After purification with AMPure XP purification kit (Agencourt) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Neuroscience 2023Quote: ... luminescence photons were collected by accumulating the image for 60 s in the presence of 2 mM D-luciferin (Promega), and the luminescence signal was measured from the same varicosities as the corresponding fluorescence image ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off with spin columns (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed using 2 μg of total RNA with M-MLV reverse transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: The activity of EBV miRs-BART 7-3p and 9-3p was evaluated in Akata-EBV/Cas9 cells by luciferase reporter gene assay with constructs based on the psiCheck-2 backbone (Promega). 3’-UTR sequences with miRNA-binding sites (Supplementary Material ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs were detected with a YFP long probe (primers listed in Supplemental Table 2) labeled with 32P-dCTP prepared according to the manual of the Prime-a-Gene Labeling System (Promega). The blot was hybridized overnight at 42 °C with the probe before being washed with 2xSSC ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Evolutionary Biology 2023Quote: ... reverse transcription was performed using Promega’s Go-Taq 2-Step system with oligo(dT) and randomized primers as per manufacturer’s instructions (Promega, Madison, WI).
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bioRxiv - Cell Biology 2024Quote: ... 50 μL IVT reactions were carried out for 2 hours at 30°C using commercially available Nuclease-Treated Rabbit Reticulocyte Lysate (RRL, Promega) and reaction components were added in the following order ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Molecular Biology 2024Quote: 3′-RACE assays were conducted using 2 μg of total RNA extracted from HEK293 cells using an RNA isolation kit (Promega) following established protocols (Shaji ...
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bioRxiv - Immunology 2024Quote: ... The 50% tissue-culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo Luciferase assay system (Promega).
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bioRxiv - Microbiology 2024Quote: The upstream regions of the ampC and cphA genes were amplified by PCR (Supplementary Table 2) and cloned into the pGEM-T easy plasmid (Promega). The inserts were subcloned into the pRKlacZ290 vector to obtain transcriptional fusions of the lacZ gene ...
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bioRxiv - Plant Biology 2024Quote: ... The sample solutions were diluted to a final concentration of 2 M urea and 50 mM ammonium bicarbonate prior to the trypsin (Promega) digestion ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase PRE reporters were generated by inserting PRE repeats into the 3’UTR of Renilla luciferase in the psiCheck-2 dual luciferase reporter vector (Promega) (Table S7) ...
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bioRxiv - Microbiology 2024Quote: The 3’UTR of human Akt or Pin1 was amplified by PCR from fibroblast genomic DNA and cloned downstream of the Renilla luciferase gene in the psiCHECK-2 dual reporter construct (Promega) by XhoI (Akt ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The annealed oligos were ligated into the Xho I and Not I sites in the 3’UTR of the Renilla luciferase gene in the psiCHECK-2 plasmid (C8021, Promega). Each plasmid was transfected individually into HEK 293 cells with miR-891b using DharmaFECT 1 Transfection Reagent (T-2001-03 ...
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bioRxiv - Molecular Biology 2024Quote: ... then media changed to 100 µL of equilibration media consisting of Ca2+- and Mg2+-free HBSS containing 2% (v/v) dilution of the GloSensor cAMP Reagent stock solution (Promega). Cells were incubated for 2 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... RLI from transduced cells was added to cytokine-starved CTLL-2 cells with proliferation of these cells subsequently measured via Colorimetric MTS Assay (Promega). Co-culture assays were conducted by incubating SB28 tumor cells with freshly isolated NK cells (BioLegend MojoSort™ NK Cell Isolation Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular proliferation was also measured at Day 0 and Day 2 using RealTime-Glo™ MT Cell Viability Assay (Promega).
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bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
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bioRxiv - Developmental Biology 2021Quote: ... abdomens were washed with phosphate-buffered saline with 0.1% Tween (PBST) four times for 15 minutes before RNase (Promega, original concentration of 4 mg/mL) was added in a concentration of 10 μL/mL for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence.
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... The attached cells were lysed in 20 μl of 1× passive lysis buffer (1× PLB, Promega) at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with nucleotide detection reagent (Promega) in a 96-well white opaque plate and left at room temperature in the dark for another 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-GFP 1:2000 (MBL, RRID: AB_591819) rabbit anti-Halo 1:1000 (Promega, RRID: AB_713650), rat anti-HA 1:500 (Sigma-Aldrich ...