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Citations for Promega :
1751 - 1800 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from PDTO samples using Maxwell RSC simply RNA Cells Kit (Promega) and underwent quality control ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were digested with trypsin/Lys-C mix (1:25 protease: protein; Promega) at 37°C was performed with constant mixing using a thermomixer ...
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bioRxiv - Developmental Biology 2024Quote: ... Each RNA sample was reverse-transcribed with the GoScript Reverse Transcription system (A5001, Promega) using Oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA from MD samples was purified using the ReliaPrep Kit (Promega, Cat # Z6210) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested with 1:50 LysC (Wako Chemicals) and 1:50 Trypsin (Promega) relative to protein amount ...
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bioRxiv - Physiology 2024Quote: ... The samples were then diluted with 100 mM NH4HCO3 and digested with trypsin (Promega) at a ratio of 1:20 (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted to 1M urea and subjected to digestion with trypsin (Promega) overnight at 37°C at a 1:20 enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were subjected to reverse-transcription (RT) using the Go Script reagent (Promega) following manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Samples were digested overnight at 37 °C with 1 μg Trypsin/LysC mix (Promega). Spin filters were centrifuged at 14 ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were cleaned with phenol chloroform and treated with DNAse (RQ1 DNase, Promega). The RNA quality and concentration were assessed by agarose gel electrophoresis and UV spectrophotometry ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from the RNA samples using GoScript™ Reverse Transcription System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA samples were subjected to reverse transcription (M-MLV Reverse Transcriptase, Promega) to obtain cDNA of either the positive-or negative-strand of EV-A71 RNA using the forward (5’-CCTCCGGCCCCTGAATGCGGCTAAT-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... Urea-solubilized pellet was then diluted to 2M urea using NaCl/Tris solution (150mM NaCl, 50mM Tris pH 8.0) and digested overnight at 37°C with 10ug of trypsin GOLD (Promega). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... and immersed in 100 µL of growth medium and 20 µL of CellTiter 96 AQueousOne Solution Reagent (Promega Co.). After another incubation for 3 h at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega) at 4°C before incubation overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The gel pieces were then first dehydrated with 100 % acetonitrile and then rehydrated with sequence grade trypsin solution (0.6 µg, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white microplate ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by applying 20 µl of CellTiter 96® AQueous One Solution Reagent (Promega, Madison WI) to each well and incubating for 2 h at 25°C ...
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bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
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bioRxiv - Systems Biology 2020Quote: Frozen fly brains were resuspended in 100 μL of lysis solution containing 0.1% (w/v) ProteaseMAX Surfactant (Promega, USA), 50 mM ammonium bicarbonate ...
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bioRxiv - Bioengineering 2020Quote: ... Cell viability was determined using the MTS assay (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, G3580; Promega) according to the manufacturer’s instructions and was calculated as a percentage of the negative control ...
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bioRxiv - Biochemistry 2021Quote: ... An aliquot (35 μg) was subjected to enzymatic hydrolysis “in solution” either with trypsin (Sequencing Grade Modified Trypsin, Promega) or with chymotrypsin (Analytical Grade ...
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white flat bottom microplate ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
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The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... a second jar of xylene for 5 min then incubated in a series of ice-cold solutions with 0.0025% RNasin Plus (Promega): 100% ethanol for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... and in-solution protease digestion using either a trypsin/Lys-C mix or Glu-C (Promega, Madison, WI, USA). Following RapiGest hydrolysis and desalting ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with 0.1M ammonium bicarbonate solution to a final concentration of 1% sodium deoxycholate before digestion with trypsin (Promega) at 37°C overnight (protein to trypsin ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Cell proliferation experiments were carried out using the CellTiter 96 AqueousOne Solution Cell Proliferation Assay reagent (Promega, Madison, WI). For these experiments ...
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bioRxiv - Cancer Biology 2022Quote: The cell viability assay was done using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Milano, Italy). It is a colorimetric method for accessing the number of viable cell in proliferation ...
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bioRxiv - Cancer Biology 2022Quote: The cell proliferation of U87MG and H4 was observed using CellTiter 96® AQueous One Solution Proliferation Assay (Promega). Cells were cultured in a 96-well plate for 72 hours under activation and AZD8055 treatment conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell viability was monitored using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA). VeroE6 cells or Huh7.5 cells were plated at 10,000 or 8,000 cells per well of flat bottom 96-well plates ...
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bioRxiv - Zoology 2020Quote: ... Other sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured by MTS/PES One Solution Cell Titer Assay following manufacturer’s protocol (Promega Corp, Madison, WI).
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bioRxiv - Plant Biology 2020Quote: ... The digestion was performed by adding 20 μL of trypsin solution (50 ng/μL; V5111, Promega, Madison, WI, USA) prepared in 50 mM ammonium bicarbonate ...
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bioRxiv - Immunology 2020Quote: ... Enzymatic digestion of proteins was performed at 37°C overnight by incubating the gel pieces in 25 ng/mL pH 8.5 trypsin solution (Sequenced Grade Modified Trypsin, ref V511A, Porcine, Promega). Digested peptides were extracted by incubation in 70% and 100% acetonitrile for 20 min each ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Neuroscience 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega) at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... a colorimetric MTS assay was performed on HUVECs following the manufacturer’s protocol (CellTiter 96 AQ ueous One Solution Cell Proliferation Assay, Promega) in 96 wells ...
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bioRxiv - Cell Biology 2020Quote: ... then diluted to a final concentration of 1 M prior to in-solution digestion with 0.2 µg/µl trypsin (Sequencing grade (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... then rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was quantified using an MTS assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Data was generated as means of six replicates from two independent experiments +/- SEM.
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bioRxiv - Microbiology 2022Quote: ... Dual Luciferase reporter assay kit and CellTiter96 Aqueous one solution cell proliferation assay kits were from Promega (Madison, USA). Non-targeting siRNA (Catalog no ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Data was generated as means of six replicates from two independent experiments +/- SEM.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... gel pieces were dried again and incubated with a freshly prepared solution of 12.5 ng/µl trypsin (sequence grade, Promega) in 50 mM Ambic for 16-20 h at 37°C ...
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bioRxiv - Physiology 2023Quote: ... neutralized with 1M Tris solution and lysates were further processed for measurement using the Glucose-Glo Assay (Promega, GmbH) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega). All experiments were performed in biological triplicates.
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...