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Citations for Promega :
1601 - 1650 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Dry gel pieces were digested with 200 μL of mass spectrometry-grade trypsin solution (4 ng/μL, Promega) in AMBIC and incubated at 37°C for 18 h ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide solution was then acidified to 0.1% formic acid before adding 0.2 μg Mass Spec grade ProAlanase protease (Promega) and incubating at 37°C for a further 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... cells were pelleted and resuspended in the Homogenization Solution of Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) and stored at -80°C until quantitative PCR experiments.
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bioRxiv - Biophysics 2024Quote: ... Ninety microliters of the oocyte supernatant were added to 40 µl of luciferase-luciferin solution (Promega, Madison, WI) for assaying luciferase activity ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). For linear RNA depletion ...
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bioRxiv - Cancer Biology 2023Quote: ... 72-hour viability assays were performed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, G3580) following manufacturer recommendations and 490nM absorbance measured using a CLARIOstar plate reader (BMG Labtech) ...
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bioRxiv - Cell Biology 2023Quote: ... We then mixed the cell solution with 80 μL of NanoGlo luciferase assay reagent (Promega, Cat. No. N1110), transferred it to a 96-well plate well (PerkinElmer,Cat ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation (MTS) Assay (Promega, Switzerland). One day before treatment ...
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bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Microbiology 2024Quote: ... the MTS solution (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (Promega # G3581) was added to individual wells ...
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bioRxiv - Cell Biology 2024Quote: ... which was followed by the addition of 25 μL of a 5X solution of NanoGlo substrate (Promega, #N2161). The cells were then transferred to a white non-binding surface (NBS ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was allowed to cool before the addition of 4µl of 4mg/mL RNase A Solution (Promega) before incubation at 37°C for a further hour ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondrial respiratory chain activity was measured daily using CellTiter 96 AQueous One Solution Reagent (Promega #G3580, Madison, USA) and a microplate reader (iMark ...
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bioRxiv - Microbiology 2020Quote: ... The beads were then washed 3 times and incubated with 30ul lysis buffer (Promega). Then 15μl of the lysate was used to measure bound NanoLuc activity.
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bioRxiv - Molecular Biology 2020Quote: ... before they were lysed in a passive lysis buffer (E1910, Promega, Madison, WI, USA) for the dual-luciferase assay ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in PBS twice and lysed in passive lysis buffer (Promega, #E194A) for 15 min on rocker ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed in 75 µl 1x Passive Lysis Buffer (provided in Promega E1960). Luminescence intensity was measured from 10 µl aliquots in technical duplicates using 50 μl of each luciferase assay reagent from the kit.
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bioRxiv - Molecular Biology 2021Quote: ... cells were then harvested and lysed with 25μL of 1×passive lysis buffer (Promega) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Cells were harvested 24–48 h after transfection/infection in passive lysis buffer (Promega). The samples were prepared according to the manufacturer’s protocol (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein pellets were resuspended in 8 M urea buffer combined with protease inhibitor (Promega) and purified using the chloroform-methanol precipitation method (Wessel & Flügge ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed by the addition of 30μl of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Microbiology 2021Quote: ... after which the media were removed and 35 µl of Passive Lysis buffer (Promega) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed by the addition of 30 µL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed in 50 µL of 1X Luciferase Cell Culture Lysis Buffer (Promega). For Gaussia luciferase assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested 48 hours after transfection using Reporter Lysis Buffer (Promega, Madison, WI), unless treated with 10nM E2 ...
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bioRxiv - Biochemistry 2020Quote: ... and were harvested on day 3 by scraping into passive cell lysis buffer (Promega). Luciferase activity was then measured using a commercial dual luciferase assay kit (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... Staining was performed by incubating embryos in staining buffer (pH9.5) containing NBT (Promega, S380C) at 50μg/mL and BCIP (Promega ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed by the addition of 30 μL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Microbiology 2020Quote: ... transfected cells were lysed in buffers provided with the Dual Luciferase assay kit (Promega). Protein concentrations were determined (Bio-Rad Protein Assay System) ...
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bioRxiv - Microbiology 2020Quote: ... followed by lysis with 200 μl of reporter lysis buffer (Promega, Inc. Madison, WI). 40 μl of the total protein lysate was mixed with 25 μl of luciferase assay reagent ...
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bioRxiv - Systems Biology 2020Quote: ... and finally with 50 mM ABC buffer supplemented with 0.0025% ProteaseMax (Promega, Cat# V2071) to facilitate sedimentation of agarose resin ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) and three freeze-thaw cycles ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) and one freeze-thaw cycle ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were harvested 24 h after infection in Reporter Lysis Buffer (Promega, E397A), lysed by freeze thawing at -80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 200μl 1X passive lysis buffer (Promega, Madison, WI, USA). All values are shown as the mean ± SEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were lysed in 12.6 μl of 1 × Passive Lysis Buffer (PLB, Promega) and light emission was measured following injection of 25 μl of either Renilla or Firefly luciferase substrate ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed by the addition of 30 µL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed by the addition of 30 µL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Microbiology 2020Quote: ... sedimented viral pellets were resuspended in 0.1 %BSA/PBS or Passive Lysis Buffer (Promega). ATP-free or ATP-containing (150 μM ATP ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... culture supernatants were removed and 1× lysis buffer was added (Luciferase assay system, Promega) to the cells in the wells ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed by the addition of 30 µL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 were transduced for 48 hours before lysis with reporter lysis buffer (Promega). Luciferase luminescence was read on a FLUOstar Omega plate reader (BMF Labtech ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which 25 µL assay buffer supplemented with 1:25 furimazine substrate (Promega, N246A) and 1:250 goat anti-rabbit Alexa Fluor 594 was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of this reaction was diluted in 100uL of passive lysis buffer (Promega). Following this ...
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bioRxiv - Molecular Biology 2022Quote: LNCaP/Luc or transfected COS-1 cells were lysed with Reporter Lysis Buffer (Promega). Luciferase activity was assayed using the Steadylite Plus kit (PerkinElmer) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed by the addition of 30 μL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed by the addition of 30 μL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed by the addition of 30 μL of passive lysis buffer (Promega) and three freeze-thaw cycles ...