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Citations for Promega :
1751 - 1800 of 2550 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized at 42 °C for 60 min with M-MLV-RT (Promega) using gene-specific primer SP1_nbdA ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Genomics 2021Quote: ... Samples were then treated for 1 hour at 37°C with RQ1 DNase (Promega) to remove possible contamination by DNA ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were digested overnight at 37 °C using Trypsin Gold (Mass Spectrometry Grade, Promega). After digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then diluted to 4M urea and digested with Lys-C (Promega), 67:1 (protein ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... for 15 minutes at 37°C before lysing with reporter lysis buffer (Promega, E397A). Samples were spun at 15,000 rpm for 10 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein digestion was accomplished by overnight incubation at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Biophysics 2022Quote: ... The protein was digested at 37 °C overnight by adding sequencing-grade trypsin (Promega) at a 24:1 substrate-to-enzyme ratio ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in fresh C+Y medium and 1 % NanoGlo substrate (Promega) was added and luminescence was measured 20 times every 1 min in a plate reader (VarioSkan luminometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were digested overnight at 37 °C with 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then digested by Lys-C (Princeton Separations) and trypsin proteases (Promega) (Florens et al. ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Molecular Biology 2022Quote: ... 2ul/5-10mg DW of Trypsin/Lys-C (Endoproteinase LysC) Mix (Promega, Cat #V5071) was added to samples and incubated with continuous agitation at 150 rpm for overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were digested overnight at 37°C with 10 nG MS Grade Trypsin (Promega). Trypsinization was quenched with 1 μL of 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the assay was performed at 20 °C using a Glomax 20/20 luminometer (Promega) by the luciferin/luciferase system supplemented with 25 µM P1,P5-di(adenosine-5’ ...
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bioRxiv - Systems Biology 2023Quote: ... washing, and digestion with lysyl endopeptidase (Lys-C, Wako) and sequencing-grade trypsin (Promega). The capture and digestion steps were performed with a Versette liquid handling robot (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were incubated at 25°C for 5 days before CellTiter-Glo assays (Promega) were performed ...
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bioRxiv - Cell Biology 2023Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biochemistry 2023Quote: Tryptic digestion was performed at 37° C with 125 μL Trypsin (Promega Cat# V511X) in digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at an enzyme to protein ratio of 1:40 ...
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bioRxiv - Synthetic Biology 2023Quote: ... or zero min at 37°C before addition of Nano-Glo substrate (Promega N1110) in 80 µL ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... followed by digestion with benzonase at 37 °C for 1 h and trypsin (Promega) digestion overnight at the same temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... after which samples were digested overnight at 37 °C with trypsin (Promega, Fitchburg, WI,) in a 1:60 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then diluted to 3M urea and digested with Lys-C (Promega) for 4 h at 25°C ...
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bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...
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bioRxiv - Cancer Biology 2024Quote: ... for 10 minutes at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega). DNase-treated RNA was purified with the RNA Clean and Concentrator-25 Kit (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... The protein solution was further diluted down to less than 2 M urea with 50 mM Tris-Cl (pH 8) and incubated with 2 μg of modified trypsin (w/w, 1∶50) (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... S2-CP8 cells were then transiently transfected with both mRFP-FKBP-5-ptase-dom and PM-FRB-CFP (0.5 μg/well of a 6-well plate for each vector) with ViaFect (Promega Corp.). After 24 h culture ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mm cell culture dishes were seeded with cells 24 hours prior to transfection and transfected the following day with the pCAGIG-Emx2 plasmid using FuGENE 6 transfection reagent (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... the whole brain of postnatal day 6 (P6) animals or dorsal raphe tissue punches (adult animals) were homogenized with Passive Lysis Buffer (Promega) and proteinase inhibitors (cOmplete ULTRA Tablets ...
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bioRxiv - Biochemistry 2019Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours and was harvested after 48 hours ...
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bioRxiv - Biochemistry 2019Quote: ... These cells were then transfected with 50 ng of pcDNA3-STING-HA and 500 ng of either pcDNA3-FLAG-HA or pcDNA3-FLAG-HA-SLC19A1 using FuGENE 6 transfection reagent (Promega). After 24 h ...
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bioRxiv - Cancer Biology 2019Quote: Replication-deficient retro- and lentiviral particles were produced by transient transfection of 293T cells using FuGENE® 6 reagent (Promega). In brief ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were grown in two 175 cm2 flasks to ∼50% confluence and transfected with 25 μg plasmid and 75 μl FuGENE 6 in 2 ml Opti-MEM according to the manufacturers instructions (Promega). One day after transfection ...