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Citations for Promega :
1651 - 1700 of 2550 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Triplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described68 ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were digested overnight at 37°C by the addition of trypsin (Promega) at a 1:100 enzyme:substrate ratio ...
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bioRxiv - Biochemistry 2022Quote: ... Trypsin and trypsin/Lys-C proteases (MS grade) were from Promega (Madison, WI); RapiGest detergent from Waters (Eschborn ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 0.7 μg Trypsin/Lys-C Mix (MS grade, Promega) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... destained and protein digested overnight at 37°C with trypsin (Promega, Madison, Wisconsin). The digested peptide samples were desalted and concentrated using C18 zip tips (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were digested at room temperature with 0.5 μg Lys-C (Promega, # VA1170) for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (Trypsin Gold, Promega).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal) (Promega) using X-tremeGENE HP DNA Transfection Reagent (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subjected to digestion using 20 µg sequencing grade Glu-C (Promega) in 1 M urea at 37°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... the proteins were digested with 1.5 µg of Trypsin/Lys-C mix (Promega) for 16 h at 37°C (800 rpm) ...
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bioRxiv - Plant Biology 2023Quote: Two vials of trypsin/Lys-C mix (100µg, V5078, Promega, Alexandria, NSW, Australia) were dissolved into 1 mL of the resuspension buffer (50mM acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: Ni-NTA beads were firstly digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Immunology 2023Quote: The eluted HSA-IgG complexes were digested by Lys-C and trypsin (Promega) to peptides and analyzed by LC-MS/MS via HCD combined with electron-transfer/higher-energy collision dissociation (EThcD ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested using a trypsin and lys-C mix (Promega, Madison, USA) at a ratio of 100:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were then digested overnight at 37°C with Trypsin Gold (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Quadruplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described 60 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with Lys-C (Wako) and sequencing-grade trypsin (Promega, V5117) (1 μg of each in 20 μL of 50 mM TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... Mass spectrometry grade Trypsin or Trypsin/Lys-C Mix (Promega #V5111 or V5071) was added at to a final concentration of 1µg/ml and incubated for 30 min at 37°C in the flask ...
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bioRxiv - Genetics 2023Quote: ... Digestion was performed overnight at 37°C with 1 µg of trypsin (Promega). The tryptic digestion was stopped by lowering the pH of the reaction below pH 4 by adding extraction buffer (0.1% TFA 2% acetonitrile) ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... and digested overnight at 37° C in 1.5M Urea with modified trypsin (Promega) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Digestion was performed at 37 °C overnight with 1 µg of trypsin (Promega). After digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... To each column was added 0.25 µg Trypsin+Lys-C (Promega; cat. V5073) in 75 µL WB3B-60 (60% acetonitrile ...
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bioRxiv - Neuroscience 2024Quote: ... 50 mM triethylammonium bicarbonate and 1 μg of Trypsin/Lys-C mix (Promega) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... and digestion was allowed to proceed overnight at 37°C using trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
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bioRxiv - Microbiology 2022Quote: ... Plasmids with the arsC-1Fas and arsC-2Fas correct sequences were purified by miniprep (Wizard® Plus SV Minipreps, Promega, cat. n° A1360) and double digested with XhoI and HindIII (Thermo Scientific™ ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae with the same genotype were pooled (min. n=10 larvae/sample) and RNA was extracted using the ReliaPrep RNA Miniprep System (Promega AG, Dübendorf, Switzerland) following manufacturer’s user guide ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were diluted with 8 volumes of 25 mM Tris-HCl pH 8.0 followed by addition of sequencing-grade modified Trypsin (Promega Corp., Madison, WI) (0.4 μg/ sample ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pseudoviruses capable of single-cycle infection were prepared by transfecting 8×106 HEK293T/17 cells with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions as previously described46 ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Immunology 2023Quote: ... Urea concentration was further adjusted to 2M using 50mM Tris pH 8 and Lysyl peptides were additionally digested with sequencing grade trypsin (Promega, Madison, WI) in an enzyme/substrate ratio of 1:20 at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were seeded in 24-well plates at a density of 4 × 104 or 8 × 104 cells/well and co-transfected 24 hours later with 200 ng of the PsiCheck2 plasmid (Promega, cat #C8021) with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg of sgRNA construct and 1.5 μg of linearized HDR plasmid were transfected into RPE1 cells using Fugene 6 (Promega). Positive cells were FACS sorted to isolate the Ndc80-EGFP expressing cells and single clones were identified by visual inspection with fluorescent microscope ...
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bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
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bioRxiv - Developmental Biology 2019Quote: ... were injected with dsRNA throughout and frozen in Trizol on dry ice after 6 days in vitro for RNA extraction according to manufacturer’s protocol and DNAse (Promega) treatment for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.
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bioRxiv - Neuroscience 2020Quote: ... and 0.4 pmol/well of hairpin constructs (pscAAV-GFP-shFoxP1 or shCtrl) using FuGENE 6 Transfection Reagent (#E2691, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells (1.2 x 106 cells/well, 6-well format) after 24 hpi using passive lysis buffer (Promega) based on the manufacturer’s instructions ...