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Citations for Promega :
2001 - 2050 of 2721 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Plates were incubated for 30 min at 25 °C before NanoBRET Nano-Glo Substrate (Promega) was added to both control and experimental samples at a final concentration of 10 µM ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biophysics 2021Quote: ... The purified RNA was treated for 30 min at 37°C with RQ1 DNase (Promega) according to the manufacturer’s protocol and then purified using one round of each phenol/chloroform and chloroform extraction followed by precipitation using ethanol in presence of 300 mM sodium acetate pH 5.5 and GlycoBlue coprecipitant (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
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bioRxiv - Microbiology 2021Quote: ... The concentrates were treated for 30 min at 37°C with 10μL of DNAse (Promega) and 2,5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and desalted using 200 µL StageTips packed with three Empore™ SPE Disks C18 (3M ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated with a trypsin/lys-C mixture (w:w = 1:50; Promega, USA) at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37° C for 30 minutes with RQ1 RNase-Free DNase (Promega, M6101). cDNA synthesis was performed using random primers (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... After two days incubation at 37°C cell viability was assessed using CellTiterGlo (Promega, G7570) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
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bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
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bioRxiv - Immunology 2022Quote: ... Proteolytic digestion was initiated by the addition of 250 ng of endoproteinase Lys-C (Promega) and incubating at room temperature for 2 hours ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were digested overnight at 37°C using 500 ng of trypsin (Trypsin Gold, Promega). Supernatants were kept and peptides remaining in gel pieces were further extracted with 1% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were digested at 37°C overnight in 10 μg MS-grade Trypsin Gold (Promega). Sequencing grade trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega). Thereafter ...
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bioRxiv - Molecular Biology 2021Quote: ... In-solution protein digestion was performed using Lys-C (Wako Chemicals, Neuss) and trypsin (Promega). For analysis of the input samples ...
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bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Cancer Biology 2020Quote: ... rat C/EBP□-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2021Quote: Proteins on beads were digested overnight at 37°C with trypsin (Promega, Madison, WI, USA) in a 25-mM NH4HCO3 buffer (0.2µg trypsin in 20µL) ...
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bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2022Quote: ... 1B and transferred to 37°C immediately after addition of supercoiled pGemT target DNA (Promega). Reactions were incubated for 30 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Cell Biology 2022Quote: ... and then digested overnight with 300 ng of Trypsin/Lys-C Mix (Promega, Madison, WI). For on-bead digestion following SA pull-down ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were digested at 37°C initially with 0.5 µg of trypsin (Promega) per sample for 6 hours then additionally with 0.3 μg of trypsin overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Each fraction was digested for 15–18h at 37°C using ultra-grade Trypsin (Promega) at a 1:100 enzyme ...
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bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed at 37 °C with 0.66 µg Trypsin Platinum (Promega, Madison, WI) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) and mixed gently at 37°C overnight to digest proteins ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Transfection of HEK293T cells was performed at 80% confluence using 25ng of each plasmid construction mixed with 150nL of Fugene 6 (E2691, Promega, France) reagent per well ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Genomics 2019Quote: ... pSMGV Dam-V5-Lap2β (Dam-Lap2β) and pSMGV Dam-V5-EMD (Dam-EMD) were transfected using Fugene 6 transfection reagent (Promega, E2691) into the Platinum-E packaging line (Cell Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested overnight at RT with a 1:50 ratio of sequencing grade modified trypsin (0.4 μg for whole proteome samples, 6 μg for phospho samples, V511A, Promega, Fitchburg WI) in 130 μL TEAB (0.05M Triethylammoniumbicarbonate in water) ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Cell Biology 2019Quote: ... a puromycin resistance marker and specific gRNA guides against VPS35, SNX5 or SNX6 (Kvainickas et al., 2017; Simonetti et al., 2017) using FuGENE® 6 (Promega). The day after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cancer Biology 2020Quote: ... used for infection of p53-null NSCs were obtained from previously transfected DF1 chicken fibroblasts (ATCC #CRL-12203) using FuGENE 6 Transfection reagent (Promega #E2691), according to manufacturer’s protocol ...