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Citations for Promega :
1701 - 1750 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Cell Biology 2019Quote: Full-length BUB-1 and ULP-1 cDNAs were cloned into pF3 WG (BYDV) Flexi® Vectors and expressed using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). BUB-1 reactions included 35[S]-labelled methionine to allow for further detection ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Microbiology 2019Quote: ... In-gel digestion of selected protein bands or spots was performed overnight at 37 °C by incubation with trypsin (Sequencing grade, Promega, Madison, USA). Afterwards peptides were extracted as previously described [24] and samples were vacuum-dried using CentriVap Vacuum Concentrator (Labconco) ...
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bioRxiv - Bioengineering 2021Quote: ... Cell lysates were pre-cleared of any non-specific streptavidin-binding proteins by incubating with 60 µL of MagneSphere paramagnetic particles (Promega, Madison, WI) for 30 min with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... PA) and AccuMap™ low pH protein digestion kit (with trypsin and lysC) and chymotrypsin (sequencing grade) were from Promega (Madison, WI). PreScisson protease was from GenScript (Piscataway ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Protein complexes were then expressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282) ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellin proteins at a concentration of 0.5 µg/µL in 25 mM ammonium bicarbonate were digested with trypsin (Promega, Madison, WI, USA) using a protein to enzyme ratio of 30:1 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... the heated plates were allowed to cool down on ice and proteins digested by adding 1µl of 10ng Trypsin Gold (Promega, catalog number V5280) in 100mM TEAB and incubating at 37°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... stable cell line expressing the human MC4-R and a split luciferase cAMP genetic sensor based on the circularized fusion of firefly luciferase and protein kinase A regulatory cAMP binding domain (clone 22F, Promega, Madison WI) was used to determine the α-MSH-elicited cAMP responses in live cells as previously described 32 ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 or sg25 cells were transfected with a control plasmid or protein expression plasmids together with the luciferase reporter plasmids using Viafect TM Transfection Reagent (Promega, Madison, WI, USA) or Lipofectamine TM 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...