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Citations for Promega :
1701 - 1750 of 2378 citations for 6 BROMO 3 4 DIHYDROQUINOLINE 2 1H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: The apoptotic effect of MK-1775 was determined by means of caspase 3/7 activity via Apotox-Glo Triplex Assay (Promega, #G6320) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times in TBST for 15’ per wash and incubated 1 hour in HRP-conjugated anti-rabbit (Promega, W4011) or anti-mouse (Promega ...
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bioRxiv - Biophysics 2024Quote: ... labeling of TRPV1exCellHalo was completed in the imaging chamber by incubating cells with 3 μM Alexa660 Halo Ligand for 5 minutes (Promega, WI) in HBR followed by 3 minutes of continuous rinsing with HBR perfusion.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Infectivity was determined after 3 days by luminescence using a Promega GloMax plate reader following addition of BrightGlo reagent (Promega #E2620) to Calu-3 cells or FLuc Assay buffer (Nanolight #318 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
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bioRxiv - Biochemistry 2024Quote: Each biological replicate was split into two 500 uL aliquots and incubated with either 3 µg of trypsin (Promega, sequencing grade) (“surface digest” ...
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bioRxiv - Systems Biology 2019Quote: ... and 20 μL of diluted insect cell extract (containing 4 μL of ICE, 3.2 μL of 5x ICB dilution buffer and 8 units of T7-RNAP-Promega#P4074 ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2022Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Developmental Biology 2019Quote: ... Ark -/- MEFs were transfected with 4 guide pairs in order to create the desired deletion using FUGENE (Promega, E2311) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or day 4 in TZM-bl cells by measuring luciferase activity using the luciferase assay system (Promega, Madison, WI) and the microplate luminometer Monolight 3096 (BD Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
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bioRxiv - Developmental Biology 2020Quote: ... Tryptic digests were performed overnight after addition of 46 μl of 100 mM Hepes pH 7.6 and 4 μl of 1 μg/μl Trypsin Gold (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were pelleted at 20,000 × g and washed with ice cold 10 mM HCl / 90% acetone before resuspension in 60 μL 4 M Urea and 0.5 × ProteaseMax (Promega), vortexed ...
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bioRxiv - Cell Biology 2021Quote: ... at room temperature (r.t.) for 4 hours and then further digested overnight with 1:50 (w/w) trypsin (Promega) at r.t ...
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bioRxiv - Biochemistry 2021Quote: ... per well of a 96-well plate 4×104 Drosophila SR2+ cells were seeded and transfected using Fugene (Promega) according to the manufacturer’s instructions with the following plasmids ...
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bioRxiv - Immunology 2021Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Microbiology 2019Quote: ... Digestion product was cooled and transferred to a pre-chilled 15 ml tube containing 4 ml 1.1X Ligase Buffer (Promega) and 1% Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... were added alone or in combination at the indicated concentrations (0.25-4 pmol) to in vitro translation reactions (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were incubated in 400 μl 50 mM ammonium bicarbonate containing 4 μl of sequencing-grade modified trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Viability of MV-4-11 cells was assessed using the CellTiterGlo 2.0 kit from Promega (as per manufacturer instructions); viability of HPAFII cells was assessed using PrestoBlue reagent from Thermo Fisher (as per manufacturer instructions) ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Neuroscience 2023Quote: ... after which the slice pieces were fixed in 4% PFA in PBS (with 0.1 U/ml RNase inhibitor, Promega) for 15 mins and dissociated into single cells through careful trituration ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability was assessed in uninfected PBMC incubated with each PROTAC under the same conditions (1.0 µM, 4 d) using the CellTiter-Blue assay (Promega).
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bioRxiv - Cell Biology 2023Quote: The Foxo1/3/4 responsive luciferase reporter plasmid was constructed by inserting six FoxO REs (FoxO-responsive elements) into the pGL4.23 (Promega). The following sequence was used for the FoxO RE ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−N-loop-PE+N-loop- AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Cell Biology 2023Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing JF646 HaloTag ligand (20 nM; GA112A; Promega) when required ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−peptide-PE+peptide-AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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bioRxiv - Plant Biology 2023Quote: ... Two µl of cDNA (1:4 dilution) was added to a 20µl qPCR reaction using the GoTaq qPCR Master Mix (Promega) and ran on a BioRad CFX Opus96 thermocycler ...
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bioRxiv - Neuroscience 2024Quote: ... with 1 μg of Lys-C (Fujifilm-Wako) at 37 °C for 4 h followed by digestion with 0.2 μg of trypsin (Promega) and mixed gently at 37 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Proteolytic digestion was performed by consecutive digestion with LysC (0.1 µg; 4 h) and trypsin (0.1 µg; 16 h) at room temperature (Promega, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of RNA was subjected to reverse transcription using M-MLV enzyme (Promega #M1705), dNTP mix 100 mM each (BLIRT #RP65 ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Genetics 2019Quote: ... following manufacturer’s protocol at 50°C in the presence of 2 U/μl RNasin (Promega). RT negative controls in which the reverse transcriptase enzyme was omitted were set up in parallel ...
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bioRxiv - Molecular Biology 2019Quote: ... The linearized plasmid was treated with 2 units of shrimp alkaline phosphatase (Promega, Madison, USA) at 37 °C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 and 2 μL of 50 ng/μL trypsin (in 100 mM TEABC, Promega) was added to the wells with single cells and two hundred carrier cells ...