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Citations for Promega :
1801 - 1850 of 2229 citations for 6 BROMO 3 4 DIHYDROQUINOLINE 2 1H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells continued to be scanned every 8 h for a further 4 days before being assessed for the number of viable cells using Cell Titer Blue® (Promega) according to the manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 100 μL of a 1:4 dilution of Bio-glo (Promega, G7940) in PBS was added to cells ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 96 and 120 h after transfection and 4 h after adding 20 µl of CellTiter 96® AQ One Solution Reagent (Promega) to each well containing transfected TCam-2 cells.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Immunology 2021Quote: The PCR product of the targeted region within exon 4 of the TRDC gene was subcloned into the pGEM-T Easy Vector system (Promega, Germany) in accordance with the manufacturer’s protocol and then transformed into XL10 bacteria ...
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bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... digoxigenin-labeled probes were synthesized from the full-length medaka cDNA of oct-4 (stem cell marker also known as pou5f1; [38]) using pGEM®-T Vector (Promega) linearized plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
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bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
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bioRxiv - Microbiology 2023Quote: ... infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with either 0.3 μg/well of Smad4 firefly luciferase reporter plasmid constructs (pLuc366 or pLuc207) or the control pGL3-Basic vector (Promega, San Luis Obispo, USA). The renilla luciferase plasmid was also co-transfected to correct for variations in transfection efficiency (45 ng/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with ABC to a final concentration of 1 M urea and digested using trypsin (Promega, V5113; 3 μg/mL, 37°C, 16 h). After digestion ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Media was changed at 3 hpi and luciferase luminescence was measured at 24 hpi using the Nano-Glo® Luciferase Assay (Promega, Madison, USA, #N1130) kit ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Plant Biology 2020Quote: ... genomic DNA was extracted from 2-wk old seedlings using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis using two AtNIP2;1 gene specific primers and the left border T-DNA primer (all primers used in this study are described in Supplemental Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Selection was done twice for 2 weeks on medium containing 25 µg/ml G418 (Promega, Mannheim, Germany) starting 2 weeks after transformation with a 2-weeks release period in between ...
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bioRxiv - Developmental Biology 2022Quote: ... The results were calculated using the 2-ΔΔCT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or with 0.05 µg GLP-1R-SmBiT and 0.05 µg LgBit-β-arrestin-2 (Promega, Hampshire, UK) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: The entire genome was amplified by PCR using GoTaq 2× Hot Start Colorless Master Mix (Promega, USA) using overlapping primer sets (Ali et al. ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Biophysics 2021Quote: ... Constructs (2 μg) were transfected into HEK293 cells on glass coverslips using Fugene HD (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Biochemistry 2022Quote: ... urea was diluted to 1 M by adding 50 mM ammonium bicarbonate and 2 μg Trypsin (Promega) per 100 μg protein ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
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bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Molecular Biology 2022Quote: Cell proliferation was measured in BAG-1 CRISPR knockout cells or siRNA treated cell in response to vehicle (0.1% DMSO) and Thio-2 (5μM and 50μM) using CellTiter-Glo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were treated with the CellTiter-Glo 2.0 Viability Assay (1:2 v/v; Promega, Madison, WI) using the Viaflo Assist Pipetting Platform (Integra Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) was added to the sample followed by 40 µg of sequencing grade trypsin (Promega). Proteins were digested overnight rotating at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... measured using a Biotek Synergy 2 multimode plate reader and the BacTitr-Glo Cell Viability assay (Promega). There was a linear relationship between concentration and luminescence (R2 =0.998) ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...