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Citations for Promega :
1651 - 1700 of 5339 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... a PCR reaction was performed using the GoTaq® G2 Green Master mix (Promega) and primers specific for the housekeeping gene Actin1 (ACT1 ...
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bioRxiv - Plant Biology 2022Quote: ... Verification of mutation in At3G01090 was done by PCR amplification using GoTaq polymerase (Promega) at annealing temperatures 56°C and 62°C and primers specific to Kin10 (At3G01090_forward ...
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bioRxiv - Plant Biology 2022Quote: ... and Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) was used to recover the desired bands from the agarose gel ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned into the pGEM-T vector (Promega, Madison, WI, USA) and confirmed by Sanger sequencing ...
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bioRxiv - Plant Biology 2022Quote: PCR reactions were performed using GoTaq G2 Flexi DNA polymerase (Promega; Catalogue number M7805), Phusion High Fidelity DNA polymerase (New England Biolabs Ltd ...
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bioRxiv - Neuroscience 2023Quote: Probes for in situ hybridization were made by cloning PCR products into pGemTeasy (Promega). 1 μg of the linearized plasmid was transcribed in vitro using NTP labelling mix and T7 or SP6 RNA Polymerase ...
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bioRxiv - Physiology 2022Quote: ... Quantitative realtime PCR was performed using the GoTaq SYBR Green qPCR Master Mix (Promega) and gene specific primers (Extended Data Table 3.) ...
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bioRxiv - Molecular Biology 2023Quote: ... and PCR screening was performed using GoTaq Flexi DNA polymerase (Promega, Wisconsin, United States) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The cold mixture was quickly transferred to a PCR-clean up column (Promega, A9282) and centrifuged for 30 s at 14,000g ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic sites encompassing targeted guide regions were amplified by PCR using GoTaq polymerase (Promega) and sequenced ...
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Molecular Biology 2023Quote: ... The resultant PCR products were inserted into a pFN21A HaloTag CMV Flexi Vector (Promega) using SgfI/PmeI restriction enzyme recognition sites ...
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Genomics 2023Quote: The target cut sites were amplified by PCR using GoTaq G2 Green (Promega,# 9PIM782). Similar to Reding et al ...
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bioRxiv - Biophysics 2022Quote: ... which was replaced by PCR-amplified LgBit insert from LgBit Expression Vector (Promega#N2681). AgeI and SpeI restriction enzymes (NEB ...
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bioRxiv - Immunology 2023Quote: ... Unpurified PCR product was used as input to rabbit reticulocyte transcription translation system (Promega) and Nano-Glo HiBit Lytic Detection System (Promega Cat No ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified PCR products were isolated and subcloned into pGEM-T easy vector (Promega) and sequenced using Bigdye terminator v3.1 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 2x GoTaq Green Mastermix (Promega), 400nM primer-F ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR product was cloned using the pGEM®-T Easy vector (Promega, USA) before the sequencing ...
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bioRxiv - Genetics 2023Quote: ... and amplified by PCR using the GoTaq Master Mix (Promega, Charbonnières les Bains, France). We employed specific primers for PTGER1 ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR products were digested by direct addition of 0.5 µl of HindIII (Promega) and incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were quantified using the QuantiFluor™-ST Blue Fluorescence Quantification System (Promega). After Illumina PE250 library construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 16 cycles of PCR with GoTaq Flexi DNA Polymerase (Promega), purified using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR-amplified products were then cloned into the pGEM-T Easy Vector (Promega) and subjected to sequencing for further analysis.
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bioRxiv - Biophysics 2023Quote: ... a regular PCR was run on a thermocycler using Taq polymerase master mix (Promega). Gene knockout was thereby confirmed by gel electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: Quantitative PCR: Taq qPCR was performed using SYBR Green GoTaq qPCR master mix (Promega) according to manufacturer’s instructions on a LightCycler 96 SW 1.0 (Roche Diagnostics GmbH ...
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bioRxiv - Microbiology 2023Quote: The amplified products underwent purification using Quick PCR purification columns (Promega, Madison, WI, USA) and were subsequently sequenced with the Big Dye Terminator Cycle Sequencing Ready Reaction Kit on an Applied Biosystems analyzer (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... All the resulting amplicons were purified using the Wizard SV PCR cleanup system (Promega). The DNA fragment for the vector was amplified by PCR using the primer set Inv-pARP3-fwd/Inv-pARP3-rev and the pARP3 plasmid as the DNA template ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was then cloned in pGEMT-easy cloning vector (Promega, USA). Cloning and HvHsfA6a gene was confirmed by sequencing followed by BLAST search using NCBI ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified for cloning into the pGEMT vector (Promega, Madison, WI, USA). A minimum of 10 clones per PCR product were sequenced to characterize the edited alleles in the transgenic plants.
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bioRxiv - Neuroscience 2024Quote: ... genotyping PCR was performed using Go Taq Green Master Mix (Promega, Madison, WI, USA) with the following four primer sets ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with 100 μl/well of PBST and 100 μl/well of 3,3,′5,5-tetramethylbenzidine TMB (Promega, Madison, WI, USA) were added and incubated for 20 min at RT ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... At the indicated time points firefly and Renilla luciferase activities were measured using the Dual-Glo® Luciferase assay system (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... washing two times with 100 µl PBS and then lysed with 1x lysis buffer (made from 5x Promega Firefly Lysis Buffer). Plates were frozen for at least 24 hours and then thawed for 2 hours before analyzing with Promega Firefly Luciferase Kit on a luminometer.
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bioRxiv - Cell Biology 2019Quote: ... non-fluorescent blocking ligand was washed out and cells were additionally washed three times with fresh DMEM medium and incubated DMEM medium containing Janelia Fluor 646 (JF646) HaloTag ligand (Promega, GA1121) in final labeling concentration 200 nM for indicated timepoints.
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bioRxiv - Cancer Biology 2021Quote: ... Plates were then incubated at 37°C in 5% CO2 and cell viability was assessed at the indicated time points by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega) over 22 and 32 days of constant treatment (for WiDr and DiFi respectively) ...
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bioRxiv - Microbiology 2022Quote: ... the beads were washed three times and bound NanoLuc activity was measured using Nano-Glo Luciferase Assay System and a GloMax® Navigator Microplate Luminometer (Promega). To construct standard calibration curves for measurement of mAb levels in plasma ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were diluted 8 times with 0.1M ammonium bicarbonate and sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) was added ...
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bioRxiv - Microbiology 2022Quote: ... the beads were washed three times and bound NanoLuc activity measured using Nano-Glo Luciferase Assay System and a GloMax® Navigator Microplate Luminometer (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of each sample in triplicates at various time points was measured using a luminescence assay of ATP (CellTiter-Glo 2.0 Assay, Promega, Cat. #G9242) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
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bioRxiv - Cell Biology 2024Quote: ... Protein-bound magnetic beads were washed three times with 200 µl of 80% ethanol and reconstituted in 50 mM TEAB and digested with trypsin (Promega, V5111) at a 1:50 enzyme-to-substrate ratio for 16 h at 37 °C with constant shaking (1000 rpm) ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.