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Citations for Promega :
1651 - 1700 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Protein complexes were then expressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... the heated plates were allowed to cool down on ice and proteins digested by adding 1µl of 10ng Trypsin Gold (Promega, catalog number V5280) in 100mM TEAB and incubating at 37°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... stable cell line expressing the human MC4-R and a split luciferase cAMP genetic sensor based on the circularized fusion of firefly luciferase and protein kinase A regulatory cAMP binding domain (clone 22F, Promega, Madison WI) was used to determine the α-MSH-elicited cAMP responses in live cells as previously described 32 ...
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bioRxiv - Plant Biology 2024Quote: ... The Halo-DREB2D fusion protein was obtained by coupled in vitro transcription/translation for 2 h at 27 °C using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, WI, USA). Halo-DREB2D synthesis was verified by Western blot using Anti-HaloTag® Monoclonal Antibodies (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... two volumes of 100 ng/μL TRAIP expression plasmid and 150 ng/μL of TTF2 or Fusion plasmid were mixed with three volumes of TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega # L326A) and incubated at 25°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Levels of HiBiT-tagged proteins were detected via luminescence using the Nano-Glo® HiBiT Lytic Detection System (Promega, catalog number N3050). Dose response curves were fitted using non-linear regression and the data shown are from n = 3 or n = 4 independent repeats ...
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bioRxiv - Immunology 2024Quote: ... Buffer was then exchanged washing the membrane three times with 50 mM NH4HCO3 and proteins digested overnight at 37°C using trypsin (Trypsin Gold, Promega, Madison, WI) at an enzyme-to-protein ratio of 1:50 (w/w).
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bioRxiv - Molecular Biology 2024Quote: One hundred micrograms of urinary proteins from each sample were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellin proteins at a concentration of 0.5 µg/µL in 25 mM ammonium bicarbonate were digested with trypsin (Promega, Madison, WI, USA) using a protein to enzyme ratio of 30:1 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... which were washed with water followed by incubation in TBS-T for at least 30 min and incubation in HiBiT blotting buffer supplemented with LgBiT protein (Promega, Madison, WI) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... at a cell density of 1×106 cells ml-1 using FuGENE HD reagent (Promega), grown in Sf900 II SFM media (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Media was then removed and 100 μL of 1:1 diluted DPBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... 1 M ABC (ammonium bicarbonate) and 1 μg trypsin (Sequence grade modified trypsin porcin, Promega) for denaturation and tryptic cleavage ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 1 mM ATP and 1 unit/uL RNasin® (Promega, N2515) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... at a cell density of 1 × 106 cells ml−1 using FuGENE HD reagent (Promega), grown in Sf900 II SFM media (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... were added prior trypsinization with 1 μl trypsin (1 μg/μl) (Trypsin-gold V5280 Promega) over night at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Cells received 1:1 maturation medium and CellTiter-Glo® Reagent (Promega, Madison, WI, USA). Plates were shaken at 480 rpm for 2 min followed by 10 min incubation at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then 50 uL of 1x Bright-Glo (2x diluted 1:1 in DPBS; Promega) was added to each well and incubated for 1 min ...
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bioRxiv - Neuroscience 2024Quote: Anti-HaloTag primary antibody (rabbit, 1 mg/ml, dilution 1:500; catalog no. G928A, Promega) and iFluor 647-conjugated anti-rabbit secondary antibody (goat ...
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Afterwards wells were washed again with PBS-T thrice and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, WI, United States) was added in a dilution of 1 ...
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bioRxiv - Immunology 2021Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence.
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... The attached cells were lysed in 20 μl of 1× passive lysis buffer (1× PLB, Promega) at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with nucleotide detection reagent (Promega) in a 96-well white opaque plate and left at room temperature in the dark for another 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-GFP 1:2000 (MBL, RRID: AB_591819) rabbit anti-Halo 1:1000 (Promega, RRID: AB_713650), rat anti-HA 1:500 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was quantified using a Caspase-Glo 1 Inflammasome Assay kit (Promega, Madison, WI). THP-1 cells were treated and harvested as described above ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence ...
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bioRxiv - Immunology 2024Quote: ... organoids were collected and lysed in 100 µl of a 1:1 dilution containing CellTiterGlo (Promega) and PBS ...
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bioRxiv - Cell Biology 2023Quote: ... For Non-SILAC samples, 1 µl trypsin (1 µg/µl Trypsin Gold, Mass Spectrometry Grade (Promega)) was added and samples were incubated at 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence in technical triplicate for each sample ...
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bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Plant Biology 2024Quote: ... Aliquots #1 and #2 were then treated with 1 mg/mL trypsin (Promega, Madison, WI, USA) and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Microbiology 2024Quote: Released caspase-1 enzymatic activity of was quantitated with the Caspase-Glo 1 inflammasome assay (Promega), following instructions from the supplier ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected with a 1:1 ratio of receptor construct and GloSensor22F cAMP biosensor (Promega) plasmids.
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...