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Citations for Promega :
1501 - 1550 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) or with 1 unit of RQ1 DNase (Promega; RNase-free) in 40 μL of RQ1 buffer (40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μl of Trypsin/LysC solution (100 ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
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bioRxiv - Molecular Biology 2022Quote: ... alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
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bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extracted from liver mouse and HEK293 cells were used as templates for PCR-cloning of the mouse/human promoter/intron fragments into the KpnI/MluI sites of pGL3 basic luciferase reporter vector (Promega). The glutamic acid (E ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse DNA was extracted from the ear using the Wizard® Genomic DNA Purification Kit from Promega (Madison, WI, USA), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: The luciferase reporter plasmid was generated by cloning the mouse HDAC7 promoter into the NheI site of pGL4 Luciferase reporter plasmid (Promega). The mouse HDAC7 promoter was amplified using Platinum SuperFi II DNA Polymerase (12361010 ...
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bioRxiv - Immunology 2020Quote: ... were used as primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Promega (Rabbit W401B and Mouse W402B), and the substrate ECL was detected by Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: OCA2 enhancer region was cloned from mouse genomic DNA into KpnI and XhoI sites of the pGL3 promoter vector (E1751, Promega). MITF proximal promoter was cloned into SacI and HindIII sites of the PGL4 promoter vector (E6651 ...
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bioRxiv - Molecular Biology 2020Quote: ... we isolated the sequence-paired sites from the native mouse and human Hes1 and Hes5 genes and cloned these fragments into the promoterless pGL3-Basic vector (Promega). Our synthetic promoters ...
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bioRxiv - Neuroscience 2022Quote: ... the cDNA fragments corresponding to the coding region or 3′-UTR of mouse Inka2 cDNA were subcloned into a pGEM-3Z plasmid vector (Promega) for in vitro transcription ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Cell Biology 2024Quote: ... the Deaf1 promoter region was first PCR-amplified from genomic DNAs of C2C12 mouse myoblasts and cloned into the pGL4.20 vector (Promega, E675A). ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from NMRI mouse cortical neuron cultures at DIV1 and DIV20 using the Reliaprep RNA Miniprep system (Promega) followed by deoxyribonuclease I (NEB ...
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bioRxiv - Genomics 2023Quote: Promoters were amplified from mouse genomic DNA using primers listed in table S1 and cloned into pGL3-Basic (E1751, Promega) using SacI and BglII.
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then incubated in species specific secondary HRP-conjugated antibody (anti-rabbit, Promega, W4011 and anti-mouse, Promega, W4021) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies were diluted in the same buffer and added to the cells ON at 4°C (mouse anti-HiBiT (Promega) catalog number CS2006A01 clone 30E5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins trapped in the suspension bed were digested in 50 mM ammonium bicarbonate with trypsin and endoproteinase Lys-C (Promega, V5073) at enzyme to protein ratio 1:25 in a 47 °C waterbath for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... miPEPs sequences cloned into pF25A ICE T7 Flexi vector were expressed in vitro using TnT® T7 Insect Cell Extract Protein Expression System (Promega). For cells extracts and Drosophila extracts ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Neuroscience 2021Quote: The protein lanes were excised from the gels and proteolytic digestion with trypsin/lys-C mix (V5073, Promega, Southampton, United Kingdom) was performed as described (Shevchenko et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1h ...
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bioRxiv - Cell Biology 2021Quote: ... conditions with HaloTag fusion protein expression were also incubated with 100nM of Janelia Fluor® 646 HaloTag® Ligand (Promega GA11220) for 30min prior to fixation ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 20μg protein from each of the three replicates (control and treated) was digested with 1μg of sequencing grade trypsin (Promega, Fitchburg, WI) followed by DTT and iodoacetamide treatment ...
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bioRxiv - Biochemistry 2021Quote: ... import assays of the comparatively higher molecular weight ACE2 and S proteins (50 μL reactions) were both performed using the TNT® Coupled Transcription/ Translation system (Promega) for 90 min at 30°C as described by the manufacturer (∼50 ng/μL cDNA,1 μM Ipom-F or an equivalent volume of DMSO ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro kinase assays were performed by co-expressing the substrate together with a gate-keeper engineered phot1 (phot1GK) using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) in the presence of 10 μM FMN ...
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bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... subjected to SP3 protein clean up as previously described (87, 88) and eluted in AmBic 50mM prior digestion with 300ng trypsin (Promega V5280). For the on-chromatin interactors ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... mRNA for each chimeric Lep protein was produced from plasmids using the RiboMAX RNA production system in accordance with the manufacturer’s instructions (Promega, Madison, WI). Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: All wheat germ cell-free expression used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...