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Citations for Promega :
1551 - 1600 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega, cat. no. L4140) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Microbiology 2024Quote: ... the protein pellets were resuspended in 100 µL 0.6M urea in 100mM TEAB buffer and 4 µg trypsin (Promega, Cat#V511B) was added for digestion at 1:25 enzyme:protein at 500 rpm at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... The FLAG-tagged VRS5 and GFP were expressed in-vitro using 2 µg plasmid and TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). Expressed FLAG-tagged proteins were bound with prepared DNA libraries for 2 hours at RT followed by immobilization by anti-FLAG magnetic beads (SIGMA) ...
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bioRxiv - Molecular Biology 2024Quote: ... The band corresponding to streptavidin was discarded and the remaining proteins were digested in-gel using modified trypsin (Promega, sequencing grade) as previously described (51) ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro expression of Halo-BnC07MYB3a fusion proteins was performed using the TNT SP6 Coupled Wheat Germ Extract System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The iodoacetamide was discarded and the gel bound proteins were digested with 0.5 ml of 20 ng/µl trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate buffer at 37 °C with constant mixing for 12 h ...
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bioRxiv - Immunology 2024Quote: ... Beads were resuspended in ammonium bicarbonate and proteins digested on the same day using 0.5 ug Sequencing Grade Modified Trypsin (Promega, Madison, WI) overnight at 37°C with agitation ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µL at a minimum concentration of approximately 100 ng/µL of each PCR amplicon was used to express TF proteins in vitro using the TnT T7 Quick for PCR DNA System (Promega L5540) with an incubation at 30⁰C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Cloning of the mNeonGreen fused shelterin proteins was performed by Gibson assembly into pRK2 backbone (original pHTN HaloTag CMV-neo, Promega #G7721).
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bioRxiv - Cell Biology 2024Quote: ... Radiolabeled rat ornithine transcarbamylase (OTC) precursor protein was synthesized from plasmid OTC with the TNT Quick Coupled Transcription/Translation kit (Promega #L2080). Upon import into the mitochondrial matrix ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA (1 µg) was treated with RNase-free RQ1 DNase 1 (cat. # M6101, Promega) with a murine RNase inhibitor (cat ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 μg of Lys-C and 1 μg of Glu-C (Promega, U.S.A.) were used for the sample for quantifying S182 phosphorylation.
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bioRxiv - Microbiology 2022Quote: ... caspase-1 activity was measured using the Caspase-Glo 1 Inflammasome Assay kit (Promega) per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... biotinylated OVA was mixed in a 1:1 ratio with SA (Promega, WI, USA) and emulsions were prepared as described ...
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bioRxiv - Genomics 2020Quote: ... pCGL-1-SNORD96A or empty pCGL-1 plasmid using Fugene (Promega, Madison, WI, USA). Cells were harvested 24- and 48-hours post-transfection for RNA isolation (TRIzol ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate) (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was mixed 1:1 with ONE-GloTM Luciferse Assay Buffer (Promega), and the luminescence was measured with the Synergy HTX Multi-Mode Reader ...
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bioRxiv - Microbiology 2024Quote: ... Briefly, 100 µl elution buffer 1 (100 mM ammonium bicarbonate, 1 µg trypsin (Promega)) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: The PD-1/PD-L1 immune checkpoint bioassay (PD-1/PD-L1 Bioassay, Promega) was performed according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent digestion was performed with Trypsin/LysC solution [100ng/μl 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested with 1:50 LysC (Wako Chemicals) and 1:50 Trypsin (Promega) relative to protein amount ...
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bioRxiv - Developmental Biology 2021Quote: α-βgal (1:100, Promega, Z3781) was used to visualize rP298.lacZ ...
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bioRxiv - Systems Biology 2022Quote: ... 0.5μl 1% Digitonin (Promega G9441), 9.5μl H2O ...
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bioRxiv - Neuroscience 2020Quote: ... fLuc (1:1000, G7451, Promega), eIF2D (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Tuj1 (1:1000, Promega G7121), H4K8ac (1:1000 ...
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bioRxiv - Zoology 2021Quote: ... 1 μl 10mM dNTPs (Promega), 2.5 μl of each primer at 10 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and 1% nanoluciferase substrate (Promega), were incubated in 384-well plate (Nunc ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM UDP-Gal (Promega) for B3GALT2 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 µl 1% Digitonin (Promega), 0.5 µl 10% Tween-20 (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 µl 1% Digitonin (Promega) (resuspension buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 U/μl RNasin (Promega) and 1 mM ATP at 16°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Furimazine (1:20 dilution, Promega) was then added and the plate promptly transferred to a Flexstation 3 plate reader at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate (1:10,000) (Promega). The experiments were repeated three times independently with similar results.
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of RNaseOUT (Promega) and 1 μL of reverse transcriptase (M-MLV RT ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% Digitonin (Promega, G9441) (final conc ...
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bioRxiv - Neuroscience 2022Quote: ... and RNasin (Promega, 1:100) on ice gently ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µl 1 % Digitonin (Promega), 0.5 µl 10 % Tween-20 (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... and 1 μg Trypsin (Promega) was added to the columns ...