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Citations for Promega :
1601 - 1650 of 2354 citations for ssc mir 487b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real time PCR was carried out using the GoTaq qPCR Master Mix (Promega) and LC480 detection system (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were digested using restriction enzymes NcoI and XhoI (Promega, Madison, WI, USA) and ligated into pre-digested expression vector pET28a ...
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bioRxiv - Microbiology 2021Quote: ... real-time quantitative PCR using the GoTaq qPCR Master Mix (Promega, Madison, WI, USA) was performed targeting a 158 bp stretch of the sodB gene using primers specific for the symbiont (sodB-F ...
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bioRxiv - Microbiology 2019Quote: ... which was then purified using the Wizard Gel and PCR Clean-Up System (Promega) and submitted to a third party for sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription-PCR was performed using a cDNA synthesis kit (Promega, Madison, WI, USA) and 2 μg of total RNA according to the manufacturer's protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... This cDNA was then used for PCR with GoTaq G2 Flexi Polymerase (Promega, M7801), using a forward primer annealing to the 3’ UTR of the mRNA of interest (all primer sequences in Supplementary Table 5 ...
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bioRxiv - Cell Biology 2019Quote: ... gel purified-PCR products were ligated into NheI/NcoI-digested pGL3-basic vector (Promega) immediately upstream of the luciferase start codon ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal quantities of tumor genomic DNA were amplified by PCR with GoTaq (M7123, Promega) using the gene-specific primers listed ...
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bioRxiv - Developmental Biology 2019Quote: PCR amplification was performed using GoTaq G2 Hot Start Green Master Mix kit (Promega) in a 25 µL standard reaction mix and the following program ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting PCR product was subcloned into the promoterless pNL1.1[Nluc] vector (Promega, # N1001) using OE-PCR as described by Bryksin and Matsumura (24) ...
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bioRxiv - Cell Biology 2021Quote: PCR fragments were cloned into the pGEM-T Easy plasmid (Promega Corporation, Madison WI) under the conditions established by the manufacturers and E ...
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bioRxiv - Genomics 2021Quote: ... PCR was performed according to standard procedures using GoTaq Colorless Master Mix (Promega, M7832) on sperm ...
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bioRxiv - Cancer Biology 2020Quote: ... The Alu PCR product was cloned into using pGEM-T Easy Vector (A1380, Promega). The clones obtained were subjected to Sanger sequencing for verification ...
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bioRxiv - Molecular Biology 2020Quote: The PCR mix included 10 µl Green GoTaq DNA polymerase mix (Promega, Madison, USA) with 200 nM of each primer and 10 ng genomic DNA ...
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bioRxiv - Plant Biology 2020Quote: ... Cloning of The PCR products were performed in the vector pGEM®-T (Promega) containing the lactose operon and the gene coding for ampicillin resistance and then transformed into chemocompetent bacteria (DH5 ...
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bioRxiv - Molecular Biology 2020Quote: ... and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712). Clones that tested positive by PCR were further validated by western blotting for eS25 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR was run for 30 cycles using GoTaq® G2 Green master mix (Promega) and specific primers ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction using the Wizard SV gel and PCR clean-up system (Promega). The purified candidate genes were cloned into pCR8/GW/TOPO entry vectors (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR product was cloned into the pGEM-T Easy vector system (Promega) and then digested with the restriction enzymes ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified and cloned into the pGEMT vector (Promega, Madison, WI, USA). pCRC and each gene coding fragments were linked by a double digestion with XhoI and a specific restriction enzyme which cuts within the pGEM-T polylinker (AatII or SacI depending on the pCRC and coding sequences orientation in the pGEM-T ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed according to standard procedures using GoTaq Colorless Master Mix (Promega, M7832) on sperm ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega. All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... DNA was purified with the Wizard® SV Gel and PCR Clean system (Promega). The DNA concentration of amplicon was measured by NanoDrop (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... were purified using Wizard® SV Gel and PCR Clean-Up System (Promega, USA), and then directly sequenced in both directions by Macrogen Inc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were purified and cloned into a pGEM-T Easy vector (Promega, A1360) according to the manufacturer’s instructions and the identity of inserts confirmed by sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... AS-PCR for each allele was assembled using the standard GoTaq DNA polymerase (Promega) protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR was done with GoTaq2 polymerase following the instructions of the manufacturer (Promega) and the following PCR protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... All LDI-PCR products from each sample were pooled and quantified using Quantus (Promega). 5-20 ng of the pooled PCR product was analyzed using Tapestation (Agilent) ...
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bioRxiv - Plant Biology 2022Quote: ... a PCR reaction was performed using the GoTaq® G2 Green Master mix (Promega) and primers specific for the housekeeping gene Actin1 (ACT1 ...
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bioRxiv - Plant Biology 2022Quote: ... Verification of mutation in At3G01090 was done by PCR amplification using GoTaq polymerase (Promega) at annealing temperatures 56°C and 62°C and primers specific to Kin10 (At3G01090_forward ...
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bioRxiv - Plant Biology 2022Quote: ... and Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) was used to recover the desired bands from the agarose gel ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned into the pGEM-T vector (Promega, Madison, WI, USA) and confirmed by Sanger sequencing ...
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bioRxiv - Plant Biology 2022Quote: PCR reactions were performed using GoTaq G2 Flexi DNA polymerase (Promega; Catalogue number M7805), Phusion High Fidelity DNA polymerase (New England Biolabs Ltd ...
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bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Probes for in situ hybridization were made by cloning PCR products into pGemTeasy (Promega). 1 μg of the linearized plasmid was transcribed in vitro using NTP labelling mix and T7 or SP6 RNA Polymerase ...
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bioRxiv - Physiology 2022Quote: ... Quantitative realtime PCR was performed using the GoTaq SYBR Green qPCR Master Mix (Promega) and gene specific primers (Extended Data Table 3.) ...
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bioRxiv - Molecular Biology 2023Quote: ... and PCR screening was performed using GoTaq Flexi DNA polymerase (Promega, Wisconsin, United States) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The cold mixture was quickly transferred to a PCR-clean up column (Promega, A9282) and centrifuged for 30 s at 14,000g ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic sites encompassing targeted guide regions were amplified by PCR using GoTaq polymerase (Promega) and sequenced ...
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Molecular Biology 2023Quote: ... The resultant PCR products were inserted into a pFN21A HaloTag CMV Flexi Vector (Promega) using SgfI/PmeI restriction enzyme recognition sites ...
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Genomics 2023Quote: The target cut sites were amplified by PCR using GoTaq G2 Green (Promega,# 9PIM782). Similar to Reding et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... amplicons were purified with a WizardTM PCR Clean Up kit (Promega, Fitchburg, WI, USA). PCR products were sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... which was replaced by PCR-amplified LgBit insert from LgBit Expression Vector (Promega#N2681). AgeI and SpeI restriction enzymes (NEB ...
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bioRxiv - Immunology 2023Quote: ... Unpurified PCR product was used as input to rabbit reticulocyte transcription translation system (Promega) and Nano-Glo HiBit Lytic Detection System (Promega Cat No ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified PCR products were isolated and subcloned into pGEM-T easy vector (Promega) and sequenced using Bigdye terminator v3.1 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 2x GoTaq Green Mastermix (Promega), 400nM primer-F ...