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Citations for Promega :
1551 - 1600 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... pTet-O-GOI-puro using the LigaFast Rapid DNA Ligation System (Promega) and heat-shock transformed into competent E ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Leukemia burden was assessed using non-invasive bioluminescence imaging by injecting mice intraperitoneally with 75 mg/kg d-Luciferin (Promega), anesthetizing them with 2–3% isoflurane ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2020Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
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bioRxiv - Cancer Biology 2019Quote: ... all mice were intraperitoneally injected (150 mg/kg) with a 30 mg/ml solution of D-luciferin (Vivo Glo Luciferin, P1043, Promega) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was isolated 24 hours after transfection and the pattern of alternative splicing was assessed by RT (with oligo-d(T) / random hexamer) and AMV Reverse Transcriptase (Promega) and PCR with GoTaq (Promega ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Bioluminescent imaging was performed to verify disseminated tumor establishment in mice by injecting D-luciferin subcutaneously at 75 mg/kg (Promega) and imaged with the IVIS Spectrum Imaging System (Perkin Elmer) ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using d-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of nanobody normalized to luciferase readout in the absence of nanobody ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase3 / 7 Kit (Promega company).
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Physiology 2019Quote: ... Plasmid DNA (6 µg) was mixed (1 : 3 ratio) with transfection reagent (Fugene 6; Promega, UK) in reduced serum media (OptiMEM ...
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bioRxiv - Developmental Biology 2019Quote: ... Nano-Luc activity was measured after 3 hours using the Nano-Glo® Luciferase Assay (Promega) and imaged with the IVIS Lumina LT system ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with Galectin-3-GFP plasmid (0.5 µg) using FuGene HD (Promega, Madison, WI) with a ratio of 3:1 for 24 h at 37 °C in 5% CO2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin (Promega Sequencing Grade; Madison, WI) per sample ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was combined at a 3:1 ratio of FuGENE® 6 Transfection Reagent (Promega) to DNA and incubated 20 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: Lentivirus particles were collected from HEK293T cell supernatant 3 days after co-transfection (FuGENE 6, Promega) of lentiviral plasmid constructs (Supplementary Table 2A ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of translation reaction was added to 30 μL Bright-Glo Luciferase assay reagent (Promega) and the resulting luminescence was measured in the illuminometer over a spectral wavelength of 350–650 nm and an integration time of 10 s at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Luminescence was determined after 3 days of culture using the GloMax® microplate reader (GM3000, Promega) and normalized with seeding control plate.
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bioRxiv - Microbiology 2021Quote: ... Infectivity was determined after 3 days by luminescence following addition of BrightGlo reagent (Promega PR-E2620). IC50 were calculated by nonlinear regression analysis using GraphPad Prism (version 9.0 ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: The 3’UTR of the genes of interest was cloned in the pSI-Check2 vector (Promega) using the primers listed in Supplementary Table S6 ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Cell Biology 2023Quote: NRK cells were transfected with IP3R-3 siRNA (see below) using Transfast (Promega Corp.; Madison WI) using manufacturer’s instructions ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Molecular Biology 2022Quote: ... 2ul/5-10mg DW of Trypsin/Lys-C (Endoproteinase LysC) Mix (Promega, Cat #V5071) was added to samples and incubated with continuous agitation at 150 rpm for overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were incubated at 25°C for 5 days before CellTiter-Glo assays (Promega) were performed ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pulsed for 4 h with CellTiter 96 Aqueous One solution reagent (Promega). Cell viability was measured by a luminometer ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosome 4: 101,716,750-101,718,250 forward strand) was cloned into pGL3-basic vector (E1751, Promega). Reporter vector ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 before adding 4 µL of 0.5 µg/µL sequencing grade trypsin (Promega) and incubated over night at 37°C ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... cells were resuspended at 2×106 cells/ml in culture media containing Nano-Glo® Endurazine Live Cell Substrate™ (Promega Inc, WI, USA # N2571) and DrkBiT (Promega Inc ...
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bioRxiv - Cell Biology 2022Quote: The number of viable cells inside HTM hydrogels subjected to the different treatments for 10 d was measured with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and 22 days post-surgery on a Lago X (Spectral Instruments Imaging) after isoflurane anesthesia and intraperitoneal administration of 150 µg D-luciferin (Promega, E1605) per gram body weight to monitor bioluminescence ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells seeded in 12-wells plates coated with poly-D-lysine were transfected with 500 ng of empty vector pHTN HaloTag CMV-neo (Promega G7721) or pCEFL-HaloTag-P-Rex1 constructs and co-transfected with 500 ng of SRE-firefly luciferase and 50 ng of Renilla luciferase plasmids ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was measured after 3 days using the CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9243). Luminescence was measured for 500ms using a SpectraMax M3 Microplate Reader (Molecular Devices) ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The pellets from centrifugation at 1,500 g for 3 min were lysed in Reporter lysis buffer (Promega) containing 0.16 U µl−1 Ribosafe RNase inhibitors ...
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bioRxiv - Bioengineering 2020Quote: ... Homozygous lpat2-3 T-DNA insertional mutants were identified by PCR using Promega PCR Master Mix (Promega) and combinations of the gene specific and T-DNA left border primers pairs ...
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bioRxiv - Cancer Biology 2020Quote: ... The 3’UTR fragments were cloned in the XbaI site of the pRL-CMV vector (Promega, #E2261) (pCMV-Luc-PTCH1 3’UTR wt/mut) ...