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Citations for Promega :
1501 - 1550 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... initially for 4 hours with 1.5 μl trypsin (0.5 μg/μl; Promega) and then another 1–2 hours with 0.5 μl additional trypsin ...
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bioRxiv - Cell Biology 2023Quote: ... After addition of 4 μl of RNAsin Plus ribonuclease inhibitor (Promega N2611), the samples were spun down ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 56 °C for 30 seconds and 72 °C for 30 seconds (30 cycles) and 72 °C for 5 minutes using GoTaq DNA polymerase (Promega) and a forward primer (hChimera exon 1-F ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 to 2 µg of RNA was used for DNase treatment as per the protocol provided by the manufacturer (Promega, Madison, Wisconsin, USA). This was followed by cDNA synthesis using reverse transcriptase kits from Applied Biosystems (High capacity cDNA synthesis kit ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Biochemistry 2019Quote: ... The beads were again washed with 50 mM ammonium bicarbonate buffer and resuspended in 100 µl of fresh 50 mM ammonium bicarbonate buffer and treated with 2 µg of sequencing grade modified trypsin (Promega; Product code V5113) at 37 oC overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA samples (2 μg) were used for the synthesis of cDNA by reverse transcription using GoScript ™ Reverse Transcriptase (Promega, Madison, USA). RT-PCR was performed with a CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: Hela cells were transfected with 2 µg of HaloTag® fusion construct and 20 ng NanoLuc® fusion construct using Fugene® HD (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 105 U2OS cells were transfected with 2 µg of sgRNA and Cas9 expressing plasmid using Fugene HD reagent (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used in this study were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Physiology 2019Quote: ... The wounded area and its surrounding area (2 x 2 cm) were immediately dissected out and RNA extraction was conducted using Maxwell® RSC simplyRNA Tissue Kit (AS1340, Promega, Madison WI) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The protein solution was further diluted down to less than 2 M urea with 50 mM Tris-Cl (pH 8) and incubated with 2 μg of modified trypsin (w/w, 1∶50) (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg of RNA were used for reverse transcription into complementary DNA (cDNA) using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, Cat. no. V5117) directly on top of the column and incubating overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... beads were equilibrated in 50 mM of ammonium bicarbonate with 2 washes followed by on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed 3 times for 5 minutes in TBST or TBST-XL for mucin-2 and incubated with anti-mouse IgG HRP Conjugate (1:10000, Promega, Cat no. W4021) or anti-rabbit IgG HRP Conjugate (1:10000 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega, USA) enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... and 50µl of 0.1mM D-luciferin K+ salt before measuring luminescence in a GloMax® multi microplate reader (Promega).
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Cancer Biology 2022Quote: ... Leukaemia propagation was monitored by bioluminescence using an IVIS imaging system (Caliper) following intraperitoneal (i.p.) injection of 150mg/kg D-luciferin (Promega). For LNPs treatment of adult RG mice ...
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bioRxiv - Molecular Biology 2022Quote: ... selected Δku80 and Δku80_Δpks1 strains were plated on Sabouraud and YPD agar paltes supplemented with 0.4 mM of d-luciferin potassium salt (Promega) to confirm the expression of a functional luciferase by bioluminescence imaging using ChemiDoc MP (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Cell Biology 2019Quote: ... Survival after 3 days of treatment was measured by the use of Resazurin (Promega #G8081). Cells were incubated with Resazurin for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: FLAG-β2AR HEK 293 cells were transfected with 3 µg cAMP luciferase plasmid DNA (Promega) using Turbofect (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections required 3 μg/well and were carried out using 0.1% Fugene HD (Promega).
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bioRxiv - Biophysics 2019Quote: ... A total of 3 μg plasmid and 9 μL of FuGENE HD Transfection Reagent (Promega) were delivered into each well following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2019Quote: ... Quantitative PCR was performed in 3 technical replicates with the GoTaq qPCR Master Mix (Promega) in 384 well plates ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Genomics 2021Quote: All the reporter constructs were cloned into pGL-3 promoter plasmids (Promega, Madison, WI, USA). Fragments of the candidate RSCNEs were cloned into pGL3-promoter vector digested by BamH I and Sal □ downstream of the luciferase gene ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptosis activities were detected using substrate from the Caspase-3 Glo Apoptosis kit (Promega, WI).
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bioRxiv - Developmental Biology 2023Quote: ... The prospero 3’UTR [2131bp] was subcloned into the pmirGlo Dual-Luciferase expression vector (Promega) downstream of the firefly luciferase gene ...
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bioRxiv - Cell Biology 2024Quote: ... washed with ammonium bicarbonate (0.1M) and proteolyzed (0.4µg of LysC (Wako) for 3 hours and 1.2µg of Trypsin (Promega) overnight) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... b-gal (Promega, Z3781), p21 (BD Pharmingen ...
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bioRxiv - Genetics 2020Quote: ... Cell viability was assayed 4 days post-seeding by Cell Titer Glo (Promega) and an Envision plate reader ...
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bioRxiv - Systems Biology 2023Quote: ... 4 μL of digestion buffer containing 20 ng/μL sequencing-grade trypsin (Promega), 20 ng/μL sequencing-grade Lys-C (Wako) ...
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bioRxiv - Pathology 2023Quote: ... 0.08 µL of GoTaq2 and 4 µL of its 5x corresponding buffer (Promega), and 5 µL of boiled calibrated bacterial suspensions ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...