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Citations for Promega :
1401 - 1450 of 5634 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2020Quote: ... Cells (5 × 106) were then lysed using 100 μl of RIPA lysis buffer (Promega, WI) supplemented with 1 μl of proteinase inhibitor and 10 μl of phenylmethylsulfonylfluoride ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cancer Biology 2019Quote: ... before dilution with 120 μL 0.04 × ProteaseMax and 5 μg/μL sequencing grade trypsin (Promega) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Survival was determined after 5 days of incubation using the Cell Titre-Blue assay (Promega). All cell lines were treated with 4 and 8 µM Erastin and 3 and 6 µM RSL3 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.3 µl of Taq polymerase (5 u/µl) provided by Promega (Madison, Wisconsin, U.S.A.). Variations in MgCl2 (0.5–1 µl ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 400 μL 50 mM NH4HCO3 containing 5 μg modified sequencing grade trypsin (Promega), then transferred to a protein LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Molecular Biology 2024Quote: ... 5–10 embryos were collected at 3 hpf and lysed in Passive Lysis Buffer (Promega) containing cOmplete Protease Inhibitor Cocktail (Sigma‒Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were cleaved by overnight digestion in porcine trypsin (Sequencing Grade Modified, Promega, Wisconsin). The tryptic peptides were extracted by using 5% acetic acid in 50% acetonitrile and vacuum-dried by speedvac ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Immunology 2021Quote: ... Total protein (100 μg) extracted from each sample was digested with Trypsin Gold (Promega, USA) with the ratio of protein:trypsin = 30:1 and incubated at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... and RXRA proteins were produced using the TNT® Quick Coupled Transcription/Translation System (Promega). Fluorescent probes were synthesized by Integrated DNA Technologies and corresponded to the LXRE1 sequence with IRDye 700 attached to the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins from the gel pieces were digested with trypsin HPLC-grade (Promega AG, Dübendorf, Switzerland) overnight and the resulting peptide solutions were desalted on C18-based STAGE tips as previously described [43,44] ...
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bioRxiv - Biochemistry 2020Quote: A total of 100 μg protein was digested with Trypsin Gold (Promega, Madison, WI, USA) with the ratio of protein:trypsin = 30:1 at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins in each well were digested with 1 μg of sequencing-grade trypsin (Promega Inc.) in 75 μl of 50 mM ammonium bicarbonate and incubated overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... On-bead protein digestion was performed by adding 1 µg of sequencing grade trypsin (Promega) to the samples ...
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bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins extracted were then processed with a precipitation-based digestion protocol using trypsin (Promega) at a ratio of 1:20 as described previously(Collins et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were digested on-bead with trypsin (1.5 µg, in provided buffer, Promega Corp.) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: Halo fusion proteins were visualized in cells by staining with fluorescent Halo ligand (Promega GA1110). Cells were seeded on polyD lysine coated coverslips (neuVitro ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in the plugs where digested overnight with Trypsin (10μg/ml) (Promega, Madison, WI, USA) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed on beads using Trypsin Gold (MS grade; Promega, cat. no. V5280), as previously described in (Hughes et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... the MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). Cleavage efficiency was typically ~70-90% at 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on-bead either with sequencing-grade trypsin (1:50) (Promega, Walldorf, Germany) for 4 h at 800 rpm and 42°C or thermolysine (1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were visualized by staining the membrane with TMB substrate (Promega; Cat.NO W4121).
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bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
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bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were digested overnight at 37°C using 500 ng of trypsin (Trypsin Gold, Promega). Supernatants were kept and peptides remaining in gel pieces were further extracted with 1% (v/v ...
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bioRxiv - Synthetic Biology 2019Quote: ... The proteins in the dried gel pieces were then digested with 0.1 μg trypsin (Promega) in 20 μl ABC with incubation overnight at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...