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Citations for Promega :
1451 - 1500 of 1655 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 50°C for 60 min and 70°C for 15 min) in the presence of 10 fg of Luciferase RNA (Promega) using the SuperSript III reverse transcription kit (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence of FITC-dextran in serum was diluted to 1:10 with PBS and measured on a Promega GloMax Multi Detection System (Promega) at 490 nm excitation and 520 nm emission wavelengths ...
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bioRxiv - Cell Biology 2021Quote: ... Beyotime) for 10 min at 37°C and the other was treated with 1 U/ul RNase inhibitor (N2518, Promega). For chromatin extraction ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed 3 times for 10 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 5% non-fat dry milk -TBST for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Rescue assays were performed in media supplemented with 10%(v/v) FBS as previously described using Cell Titer 96 MTS (Promega). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... The primer and DNA marker were end-labeled with 10 μCi of [γ-32P]ATP by T4 polynucleotide kinase (Promega) and then used for reverse transcription with avian myeloblastosis virus (AMV ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Microbiology 2022Quote: ... A 5 mL sample was then mixed with 10 mL of RNAprotect and processed with the SV Total RNA Isolation System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Hana 3A cells were authenticated using polymorphic short tandem repeat (STR) at the Duke DNA Analysis Facility using GenePrint 10 (Promega) and shown to share profiles with the reference (ATCC) ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in 1×PBST and then incubated with the secondary antibody (anti-rabbit or anti-mouse IgG conjugated with horse radish peroxidase at 1:10 000, Promega). The membranes were then revealed using an ECL kit (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... The Genetic Resources Core Facility at the John Hopkins University School of Medicine authenticated the HPAF-II cells by performing short tandem repeat profiling via GenePrint 10 (Promega) and comparing to the ATCC database ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Microbiology 2023Quote: ... princeps luciferase enzymatic activity due to luciferase reconstitution was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies) using a reading time of 10 s after injection of 50 µl Renilla luciferase reagent (Promega). Mean relative light units (RLUs ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Cancer Biology 2023Quote: ... were authenticated by the Genetic Resources Core Facility at the John Hopkins University School of Medicine by performing short tandem repeat profiling via GenePrint 10 (Promega) and comparing to the ATCC database ...
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... before drying further with a speed vac until the pieces were ‘dice-like bouncy.’ The protein samples were then incubated in digestion buffer [50 mM NH4HCO3 and 10 μg/mL Sequencing grade modified trypsin (Promega)] overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were reswelled in 60 mL of 50 mM NH4HCO3 buffer containing 10 ng/mL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Genomics 2023Quote: ... The plate was then incubated at room temperature for 10 min to stabilize the luminescent signal and recordings were taken on a GloMax Discover System (Promega).
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Presumptive screening of 10 colonies per mPilA variant was performed via colony-PCR using GoTaq® Green Master Mix (Promega) and forward and reverse primers Diag:71301:MPilA:All:FWD and Diag:71301:MPilA:All:REV (SI Table S1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were maintained at 37 °C and 5% CO2.Cell lines were authenticated using Short Tandem Repeat fingerprinting carried out using the GenePrint 10 system (Promega). For 3D spheroids ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell line authentication was performed by short tandem repeat DNA analysis with the GenePrint 10 System (Promega, Fitchburg, WI, USA).
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Molecular Biology 2023Quote: ... Culture aliquots (200 µL) were taken to measure OD620 and luminescence by adding 10 µL of 1 mg/mL beetle luciferin (Promega) in a BioTek Cytation 3 Microplate Reader ...
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bioRxiv - Genetics 2024Quote: ... Cells were co-transfected after 24 hours with 50 ng of the CNTNAP5 luciferase reporter constructs along with 10 ng of pRL-TK Renilla luciferase internal control using FuGENE HD transfection reagent (Promega). At 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assays were performed by co-transfecting HEK-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (E2691; Promega) and performing the Dual-Luciferase Reporter Assay System (E1910 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... Kanamycin-sensitive exconjugants were screened for the mutant with colony PCR using altgenoF and altgenoR primers designed outside of the deletion flanks with the following PCR reaction mix: 10 µl of GoTaq Green master mix (Promega), 0.5 µl of each primer at 10 µM concentration ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of extracted DNA was added to an amplification mixture containing 1 µl (10 µM) of primers and 12.5 μl of PCR Master Mix Plus (Promega, USA). To each mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... The phase separation assay was performed in the presence or absence of 10% polyethylene glycol 8000 (PEG-8000, Promega, V3011) and the reaction mix loaded in a home-made imaging chamber consisting of a glass slide attached to a cover slip by two parallel strips of double-sided tape ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the PCR reaction included 10 µL GoTaq Hot Start Green Master Mix (Promega, Madison, WI US), 2 µL of each primer at 10 µM ...
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bioRxiv - Biochemistry 2024Quote: Kinase activity was measured in a generic kinase reaction buffer (20 mM Tris pH 7.4, 50 mM KCl, 10 mM MgCl2) using the ADP-Glo assay kit (Promega, V6930) and on a commercial CK2 substrate peptide (EMD Millipore ...
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bioRxiv - Immunology 2024Quote: ... the samples were quenched with 10 mM DTT and digested overnight at 37°C with Trypsin gold (Promega, WI, USA). The digestion was stopped and the surfactant was cleaved by treating samples with 200 mM HCl (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was executed in a total of 20μl reaction volume which includes 2 X Mix SYBR green I (10μl; Promega Corporation, Madison, WI, USA), primer (0.25μl ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was monitored every 30 seconds for 2 min using a luminometer (GloMax® 20/20n Luminometer, Promega Italia, Milan, Italy) for the luciferin/luciferase chemiluminescent method ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected according to manufacturer instruction with a transfection mix containing a ratio of 1 µg of plasmid for 2 µl FuGene HD transfection reagent (Promega, REF E2311) diluted in DMEM without FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’int-F and 24nt-6stp-R primers and 2) 3’KI: 24nt-6stp-F and 3’int-R using the GoTaq DNA polymerase mix (Promega, Madison, WI) and 200 nM of each primer ...
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bioRxiv - Molecular Biology 2021Quote: ... protein ratio of 1:50 w/w for 2 hours at 37°C and further digested with 30 µL 50 mM TEAB containing trypsin (Sequencing Grade Modified, Promega, Madison, WI) at a trypsin ...
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bioRxiv - Developmental Biology 2019Quote: ... 3’UTR sequences were cloned downstream from the renilla luciferase gene using the XhoI and NotI sites in the siCHECK-2 vector (Promega, Madison, WI). This vector also contains a firefly luciferase gene driven by an independent protomer ...
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bioRxiv - Neuroscience 2019Quote: ... Gaussia luciferase activity was measured in the dark 0.1 sec after injection of 100 μl/well of 40 mM coelenterazine, substrate (NanoLight, Pinetop, AZ) using a 2 second integration on a Veritas Microplate Luminometer (Promega, Madison, WI). The cells were subsequently assayed for red Firefly activity using the ONE-GloTM Luciferase Assay System kit (Promega ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg of purified RNA for each sample was used to synthesize cDNA using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) at 42°C for 1 h ...