Labshake search
Citations for Promega :
1401 - 1450 of 1655 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... Cysteines were then alkylated with 0.5 M iodoacetamide in 100 mM NH4HCO3 (45 minutes in a dark room at room temperature) and proteins were digested overnight with 10 ng/μl trypsin (CAT NO V5280, Promega) in 25 mM NH4HCO3 77 ...
-
bioRxiv - Molecular Biology 2019Quote: ... purified by ethanol precipitation and co-transfected into Huh7 cells in 6-well plates (90 ng reporter, 10 ng pcDNA-Blast) using FuGENE 6 transfection reagent (Promega). Cells were selected with blasticidin (0.2 μg/mL ...
-
Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Media was then removed and cells were incubated in serum-free DMEM containing either 0.49% dimethyl sulfoxide (Pierce) or a differentiation medium (serum-free DMEM supplemented with 10 ng/ml fibroblast growth factor (Promega), 240 μM isobutylmethylxanthine (Sigma) ...
-
bioRxiv - Plant Biology 2019Quote: ... 40 μL of supernatant was then transferred from each sample to PCR strip tubes and mixed with 10 μL Renilla luciferase lysis buffer (Promega). Prepared samples were then stored at −20°C until luciferase assays were run ...
-
bioRxiv - Bioengineering 2021Quote: ... Ligations of the digested plasmid backbones and PCR products occurred for 5-10 minutes at RT using T4 DNA ligase (Promega) prior to transformation into NEB® Turbo Competent E ...
-
bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... before treatment without or with OSM (10 ng/ml, 45 min) and RNA extraction (ReliaPrep RNA Cell Miniprep System, Promega). cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System ...
-
bioRxiv - Microbiology 2020Quote: ... the plate was incubated at room temperature for 10 minutes to stabilize luminescent signal before luminescence was recorded on a GloMax plate reader (Promega).
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
-
bioRxiv - Microbiology 2021Quote: ... mice were anesthetized with ketamine-xylazine (100 mg/kg ketamine and 10 mg/kg xylazine) and then retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega) diluted 1:10 in PBS ...
-
bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
-
bioRxiv - Cell Biology 2021Quote: ... the antibody–bead mixture was resuspended in 500 μl of the reaction mixture containing 10 μg of fragmented total RNA,100 μl of reaction buffer and 5 μl of RNasin Plus RNase Inhibitor (Promega), and incubated for at least 4 h at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... gel pieces were reswelled in 60 μL of 50 mM NH4HCO3 buffer containing 10 ng/μL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed for 15 minutes on ice then treated with 10 µL of RQ1 DNase (Promega, catalog no. M610A) for 5 minutes at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lines were validated by short tandem repeat (STR) DNA typing using the Promega GenePrint 10 System according to the manufacturer’s instructions (Promega #B9510). Cells were cryopreserved in Recovery Cell Culture Freezing Medium (Gibco #12648-010) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 μl of the lysate was used to measure luciferase activity by sequential mixing with 10 μl of each substrate from the Dual Luciferase Reporter Assay System (Promega). Luminiscence was measured on a Mithras LB 940 microplate reader (Berthold Technologies) ...
-
bioRxiv - Biochemistry 2022Quote: ... The gels were dehydrated with acetonitrile and rehydrated with 10 mM ammonium bicarbonate containing 13 ng µl-1 trypsin (Promega). The protease digestion was carried out for 12 h at 37 °C following which the peptides were extracted from the gels using 1:2 (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... and the Gaussia princeps luciferase enzymatic activity was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies, reading time 10 s after injection of 50 µl Renilla luciferase reagent (Promega). For the minigenome assays ...
-
bioRxiv - Plant Biology 2021Quote: ... a 10 mm × 10 mm piece of tissue surrounding the inoculation point was applied to the Dual-Luciferase Reporter Assay System (Promega), to detect the firefly luciferase activity (or activation of the ACS2 and ACS2mut promoters) ...
-
bioRxiv - Microbiology 2021Quote: ... air-dried and treated by 10 mkl of 12 mg/mL solution of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) in 50 mM ammonium bicarbonate for 15 h at 37°C ...
-
bioRxiv - Plant Biology 2021Quote: ... The proteins were then incubated for 30 min on ice in the presence of 10 μL of 20 ng/μL trypsin (Trypsin V5280, Promega), followed by 16 h at 37 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... cells were mock treated or treated with 10 unit/mL of uIFN for 6 hrs and collected in 1× passive lysis buffer (Promega). Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
-
bioRxiv - Microbiology 2021Quote: ... Five ml of BHI broth containing 10 μg/ml tetracycline was inoculated with a single colony and genomic DNA was extracted (Wizard DNA extraction kit, Promega). Genomic DNA was sequenced by paired-end joining Illumina (Biomics Platform of the Institut Pasteur ...
-
bioRxiv - Cancer Biology 2021Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by DNA sequencing ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
-
bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ten to twenty 10 μm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... Both human cell lines were validated using the cell line authentication service at Duke University and confirmed using the GenePrint 10 kit from Promega and tested to be mycoplasma-free ...
-
bioRxiv - Plant Biology 2019Quote: ... subspecies nebraskensis were carried out in 20 μl reactions containing 10 μl of 2X GoTaq® Green Master Mix (Promega), 1 μl of each forward and reverse primer (5 μM) ...
-
bioRxiv - Plant Biology 2019Quote: Semi-quantitative RT-PCR was performed on RNA isolated of 10 days-old seedlings using the total RNA isolation system (Promega). Reverse transcription was performed on 300 ng of total RNA using the iScript advanced kit (BioRad) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and activity of both luciferases was measured in 10 μL of the sample with the Dual Luciferase Reporter Assay system (Promega) on a Modulus Single Tube Reader (Turner Biosystems) ...
-
bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 40 μl of the cell suspension were transferred to each well of 96-well plates containing 10 μl of 5X GloSensor cAMP Reagent (Promega) prepared according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... was added to each well and the plate was incubated for 10 minutes at room temperature before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment for nine total measurements per cell type and treatment.
-
bioRxiv - Immunology 2021Quote: We seeded 1 × 105 of HEK293 cells into 24-well plates in triplicate and transfected the cells with an expression vector together with p-125 Luc reporter (100 ng/well) and phRL-TK (10 ng/well, Promega) plasmids ...
-
bioRxiv - Immunology 2020Quote: ... HF and MEF cell lines were transfected with 10 ng of the internal control plasmid pTK-Renilla (pRL-TK, Promega) or 60 ng of the reporter plasmid pLUC-NF-κB (R ...
-
bioRxiv - Developmental Biology 2019Quote: ... The University of Queensland) constructs were co-transfected (at ratio of 1:10) with renilla luciferase (pRL-TK vector, PROMEGA) in Non Targeting Control or H2a.z.2 siRNA treated B16 melanoma cells ...
-
bioRxiv - Immunology 2019Quote: HEK cells were seeded at 3.0 × 106 cells in 10 cm dishes and transfected with 3 μg of the indicated DNA construct using FuGENE HD (Promega). 24 h post-transfection ...
-
bioRxiv - Molecular Biology 2019Quote: ... The identity of all cell lines was confirmed by DNA finger printing analysis utilizing the GenePrint® 10 System (Promega). BT-474 and MDA-MB-453 cells were cultivated in DMEM medium (Pan-Biotech ...
-
bioRxiv - Neuroscience 2021Quote: ... or methadone were added for another 10 min followed by addition of 20 μL of the RLuc substrate coelenterazine h (Promega), at 5 μM final concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was suspended in buffer P1/RNAse A and lysed by adding 1/20 volume of 10% SDS (Promega). After incubating for 20 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... Hana 3A cells were authenticated using polymorphic short tandem repeat (STR) at the Duke DNA Analysis Facility using GenePrint 10 (Promega) and shown to share profiles with the reference (ATCC) ...
-
bioRxiv - Plant Biology 2021Quote: ... The DNA pellet was resuspended in water with RNase A (10 µg/mL) for amplicon library preparation using GoTaq G2 Green Master Mix (Promega) and primers (Supplemental Table S1 ...