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Citations for Promega :
101 - 150 of 4654 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Microbiology 2021Quote: ... ImProm-II Reverse Transcriptase (Promega) was used to synthesize cDNA from total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Genetics 2019Quote: ... Clone libraries were created for each amplified ITS region using the pGEM®-Easy Vector System (Promega). Positive inserts were verified by PCR using SP6 and T7 primers ...
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bioRxiv - Cancer Biology 2021Quote: ... containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega). Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Plant Biology 2021Quote: ... The entire protein region of the gel was excised and subjected to in-gel trypsin (Promega V511A) digestion after reduction with DTT and alkylation with iodoacetamide ...
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bioRxiv - Immunology 2021Quote: ... containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega). Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated and the gene region of interest was amplified using GoTaq DNA polymerase (Promega) (HBB Fw ...
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bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... regions of mtDNA were cloned from donor cell lines into p-GEM-T Easy vector (Promega, A1360). Samples were then diluted to specific concentrations and analyzed to evaluate probe specificity.
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bioRxiv - Microbiology 2021Quote: Isolated ovine lungs were infused with 1.5% low-melting point agarose (Promega) in DMEM and subsequently put into cold PBS in order to allow the agarose to solidify ...
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bioRxiv - Biophysics 2021Quote: ... DNA ligation products were separated on a 0.5% low melting agarose (Promega) gel and the 18.4-kb band was excised and treated with β-Agarase (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... to an agar block (4% agarose, low melting point, analytical grade, Promega, Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega). After overnight incubation at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Anesthetized zebrafish were embedded in low melting point agarose gel (Promega, V2111) in a confocal dish ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng of total RNA was transcribed to cDNA using the ImProm-II™ reverse transcription kit (Promega, A3500) with random primers ...
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bioRxiv - Plant Biology 2024Quote: ... The complete amount of RNA was reverse transcribed to cDNA using the oligodT from the GeneRacer kit and following the manufacture’s guide of ImProm-II Reverse Transcriptase (Promega). The PeSPL6 and PeSPL13a 5’-ends were amplified by PCR using the GoTaq Master mix (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Microbiology 2019Quote: ... and ImProm-II Reverse Transcriptase (Promega) was performed31 ...
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bioRxiv - Microbiology 2021Quote: ... and Improm-II reverse transcriptase (Promega) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and ImProm II reverse transcriptase (Promega). Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc. ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST region of psfRps29-GST was replaced with luciferase cDNA (pNL1.1[Nluc]Vector; Promega Madison, WI, USA). Luciferase cDNA was amplified via PCR using primers Nluc_FW2 and Nluc_RV2 ...
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bioRxiv - Developmental Biology 2022Quote: CEBPa promoter (−2kb) or enhancer region (Cooper et al., 2015) was cloned into the pGL3 basic backbone (Promega). The construct containing part of the Id1 promoter region (336 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products from the unique regions of her9 were cloned into the pGEMT-easy vector (Promega, Madison, WI). Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... pGL3basic-IFNβ-Luc (mIfnb1- FLuc) consists of the 812 bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (99) ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rRNA region was amplified with the standard bacterial primers 335F and 769R with GoTaq Mastermix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... between 0.5 to 1 µg of total RNA was reverse transcribed into cDNA using an ImProm II Reverse Transcriptase kit (Promega). Equal amounts of cDNA were quantified by RT-qPCR using the IQ SYBR green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... The plate reader was programmed to inject 40 μl of Luciferase Assay Substrate re-suspended in Luciferase Assay Buffer II and 35 μl of 1X Stop&Glo Reagent (Dual-Luciferase Reporter Kit, Promega) per well ...
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2020Quote: ... Tissues were embedded in 3% low melting point agar (Promega, Madison, WI, USA). Formalin embedding ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized using a 1% low-melting-point agarose (Promega; Cat#: V2111) solution and covered with Volvic water containing tricaine ...
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 enzyme wt:sample wt to the lavage ...
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 weight:weight to the S-Trap column ...
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bioRxiv - Systems Biology 2022Quote: ... Glu-C (Promega) or Lys-C (Wako chemicals ...
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bioRxiv - Pathology 2024Quote: ... Lys-C (Promega) was added in an enzyme:substrate ratio of 1:75 for 4 hours at 25 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...