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Citations for Promega :
1 - 50 of 4654 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Goat immunoglobulins against rabbit or mouse immunoglobulins conjugated with horseradish peroxidase (Promega) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Molecular Biology 2019Quote: ... The 3.6 kbp human CDC37 promoter region was PCR amplified and subcloned into a pGL3-luciferase vector (Promega, Madison, WI) using KpnI and BamHI ...
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bioRxiv - Molecular Biology 2023Quote: ... The ImProm-II reverse transcription kit (Promega) was used to synthesize cDNA from 1 µg of total RNA using the included oligo dT primers in a volume of 20 µL per the manufacturer’s direction ...
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bioRxiv - Molecular Biology 2021Quote: ... goat anti-rabbit immunoglobulin G conjugated with alkaline phosphatase (Promega, USA) was added to the wells and incubated for 3 h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Immunology 2021Quote: ... and then lung lobes were inflated with 3% (w/v) 45°C prewarmed low-melting agarose (Promega). The inflated lung lobes were immediately removed and cooled on ice for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Immunology 2019Quote: ... the mutated region amplified with GoTaq (Promega) (Supplemental Table 2) ...
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bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized with the ImProm II Kit (Promega). qPCR was performed using the Brilliant III Ultra-Fast SYBR® Green QPCR Master mix reagents (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... An ImProm-II™ Reverse Transcriptase System kit (Promega, USA) was used to synthesize the first strand cDNA using 1.5 μg of the DNA-free RNA sample as a template and each reaction was carried out according to the supplier’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained using an ImPROM II kit (Promega, A3800) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... low melting point (V3841, Promega). Immunofluorescence images were acquired with the iMic spinning disk confocal microscope ...
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bioRxiv - Immunology 2023Quote: ... followed by goat anti-mouse immunoglobulin conjugated to alkaline phosphatase (Promega S3721, dilution 1:7500). Focuses of infection were revealed using NBT/BCIP reagent (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: TERT promoter regions were cloned into the pNL1.1 vector (Promega), transformed in TOP10 chemically competent cells (Lifetech) ...
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bioRxiv - Developmental Biology 2020Quote: ... To localize the regions of gene expression NBT/BCIP (Promega) in alkaline phosphatase buffer (See Table S3 for composition ...
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bioRxiv - Systems Biology 2021Quote: ... 2 ul were used as templates for PCR with the srrm3 or srrm4 primers designed to amplify the genomic region of interest (listed in Table S12) and GoTaq® Flexi DNA Polymerase kit (Promega).
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bioRxiv - Evolutionary Biology 2019Quote: ... probe was generated by an in vitro transcription system using primer pairs with T7 and SP6 promoter regions (T7 and SP6 Ribomax™ large scale RNA kit, Promega) (Mlig-pro46 ...
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bioRxiv - Immunology 2020Quote: ... and total RNA isolated using the Maxwell 16 low elution volume simplyRNA Blood Kit (Promega, Madison, USA). Complementary DNA (cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Luminescence was read after adding 50μL Luciferase Assay Reagent II (Promega kit), then read a second time after addition of 50μL/well of stop and glow reagent ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was done using cDNA ImProm-II reverse transcription kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... in 0.5% low melting point agarose (Promega) for imaging in brightfield or under RFP fluorescence (Figure 7) ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Genetics 2022Quote: ... the SEV hardware was replaced with the Low Elution Volume (LEV) hardware and the Simply-RNA Cell kit (Promega) was used ...
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bioRxiv - Cell Biology 2020Quote: ... to clone the promotor region into the pGL4.10 [luc2] construct (Promega; E6651). The promotor sequence included the sequence of a transcriptional active area ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The BRh coding region was cloned into pGEM-T easy vector (Promega). A short oligonucleotide sequence encoding the 1D4 epitope of bovine rhodopsin (STTVSKTETSQVAPA ...
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bioRxiv - Cell Biology 2019Quote: ... RT reaction was carried out in 15 ul reaction mixture at 40 °C for 60 min after addition of ImProm II (Promega). DNA fragments for L chain and the variable region of H chain (VH ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was prepared using ImProm-II™ reverse transcription kit (Promega, A3800) and 10 ng of cDNA were used as template for each real-time PCR reaction ...
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bioRxiv - Pathology 2023Quote: ... We reverse-transcribed cDNA from total RNA using the ImProm II Kit (Promega, US) with Random primers ...
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bioRxiv - Biophysics 2022Quote: ... spheroplast/sucrose buffer suspension was added to 500 μL warm (42 °C) agarose solution (low melting point agarose, V2831 Promega, 2% w/v in sucrose buffer) using a cut pipette tip ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...