Labshake search
Citations for Promega :
301 - 350 of 4654 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
-
bioRxiv - Developmental Biology 2020Quote: The coding region of her9 was amplified by RT-PCR and the cDNA was cloned into a pGEMT-easy plasmid (Promega, Madison, WI). Capped mRNA was synthesized using the mMessage T7 kit (Ambion ...
-
bioRxiv - Genetics 2019Quote: The target genomic region was amplified using PCR and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The DNA sequence of the PCR fragment was determined using cycle sequencing with BigDye v3.1 reagents (Applied Biosystems ...
-
bioRxiv - Immunology 2022Quote: ... that cover the promoter region and proximal portion of the first exon of the gene was PCR amplified with GoTaq DNA Polymerase (Promega, Madison, Wisconsin) from bisulfite-converted DNA using the primer listed in Table S3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Primer sets for real-time PCR (Table 1) were used to amplify target regions from genomic DNA and cDNA as templates by the GoTaq DNA polymerase (Promega, WI, USA). For real-time PCR experiments ...
-
bioRxiv - Cancer Biology 2021Quote: ... and reverse-transcribed with ImProm-II reverse transcriptase (Promega) using oligo-dT primers ...
-
bioRxiv - Microbiology 2020Quote: ... and ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA) was performed ...
-
bioRxiv - Plant Biology 2022Quote: ... and reverse-transcribed using ImProm II reverse transcriptase (Promega) and oligo(dT)15 primer ...
-
bioRxiv - Genomics 2020Quote: ... and 1U of ImProm-II™ Reverse Transcriptase (Promega). SARS-CoV-2 genomes were quantified by using a qRT-PCR assay targeting a conserved region of the envelope gene ...
-
bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega). Two-step quantitative real-time PCR(qPCR ...
-
bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Promega, Madison, WI, USA) was diluted to 10,000 genome copies per μl then serially diluted to 10 genome copies per μl and used to create a human DNA copy number standard curve ...
-
bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human Genomic DNA Male (Promega, cat # G1471) and IDH1 R132H Reference Standard (Horizon ...
-
bioRxiv - Cancer Biology 2020Quote: ... 50ng of normal human genomic DNA (Promega) was used as a negative control ...
-
bioRxiv - Cancer Biology 2021Quote: ... Human LAMA1 cDNA was purchased from Promega and sub-cloned into pLVX-Tet-On Advanced vector (Clontech) ...
-
bioRxiv - Genomics 2021Quote: Commercial human DNA (Promega, Cat. No. G1521) was used for PCR amplification of all tested genome fragments from AF associated loci (for primers used see Supplementary Table S1 ...
-
bioRxiv - Microbiology 2023Quote: ... Human gDNA (Promega, San Luis Obispo, CA) was added to the extracted gDNA from the clinical swabs to reach a total input of 3 μg/130uL for fragmentation and library prep ...
-
bioRxiv - Immunology 2022Quote: ... These included 5μg/ml human gDNA (Promega), 500ng/ml citrullinated histones (Cayman Chemical #501620) ...
-
bioRxiv - Genomics 2023Quote: ... 1 ug human DNA (Promega, Madison, WI), 5 ug sheared salmon sperm DNA (Promega ...
-
bioRxiv - Genomics 2023Quote: ... Human male genomic DNA (Promega, CAT #G1471) was used for a standard curve at 125 pg/µL ...
-
bioRxiv - Biochemistry 2024Quote: ... a K562 human peptide digest standard (Promega) and E.coli peptide standard (Waters ...
-
bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... Digestion was performed for ∼18 hours at 37 °C using sequencing grade Glu-C (Promega). Peptides were analysed by nanoHPLC-MS/MS over a 65 min acquisition with elution from a 50 cm C18 PepMap column onto an Orbitrap Fusion Tribrid mass spectrometer via an Easy-Nano ionisation source ...
-
bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin or Lys-C alone (Promega). Digested peptides were separated by liquid chromatography and analyzed on an Orbitrap Elite mass spectrometer (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C.
-
bioRxiv - Molecular Biology 2024Quote: The cytotoxicity of venoms was assessed in Madin-Darby canine kidney II (MDCK II) cells using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, cat. no. G7570) as previously reported (Hurka et al. ...
-
bioRxiv - Molecular Biology 2022Quote: The plasmid pEGFP-C1-human Rab29 was generated by inserting human Rab29 sequence from pFN21A-Halo-Rab29 (Promega, #FHC08084) into Bgl II - Eco RI site of pEGFP-C1-rat Rab29 plasmid that was used previously11 ...
-
bioRxiv - Cancer Biology 2020Quote: ... predigested with Lys-C (Wako) (1:75, 4h at 37°C) and trypsin digested overnight (Trypsin Gold, Mass Spectrometry Grade, Promega; 1:50 at 37°C). Peptide samples were desalted using C18 Sep-Pak cartridges (3cc ...
-
bioRxiv - Cancer Biology 2020Quote: ... predigested with Lys-C (Wako) (1:75, 4h at 37°C) and trypsin digested overnight (Trypsin Gold, Mass Spectrometry Grade, Promega; 1:50 at 37°C). Peptide samples were desalted using C18 Sep-Pak cartridges (3cc ...
-
bioRxiv - Cancer Biology 2020Quote: ... Plates containing the different transfected cells were incubated for 24 hours at 37°C before the luciferase activity was measured using the Dual-Glo luciferase detection kit (Promega: E2920).
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR products with low concentration or bad sequencing quality were cloned into pGEM-T Easy Vector (Promega) for sequencing ...
-
bioRxiv - Cancer Biology 2020Quote: Cell viability was assayed in 2D-adherent and ultra-low adherent 3D-spheroids using CellTiter-Glo (Promega) as previously described.6 ...
-
bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized in the dish using a drop of 1% low-melting-point agarose (V2111, Promega) solution in 80% glycerol ...
-
bioRxiv - Molecular Biology 2019Quote: The putative LINC00662 binding regions within the ELK4 gene were amplified by PCR and cloned into downstream of pmirGLO dual-luciferase vector (Promega, Madison, WI, USA) to form the wide-type plasmid (ELK4-3’UTR-Wt ...
-
bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...
-
bioRxiv - Biophysics 2022Quote: pHalo-H2B was generated by PCR amplification of the H2B coding region from an H2B-GFP template and cloned into a pFC14A backbone (Promega, Madison, WI, USA) to fuse the HaloTag to the C-terminus of H2B (56).
-
bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
-
bioRxiv - Biochemistry 2022Quote: ... The scFv gene was then ligated into pCSE2.6-hIgG1-Fc-XP vector (43) using T4 Ligase (Promega) and transformed into E ...
-
bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
-
bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
-
bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
-
bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
-
bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
-
bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
-
bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was made using Improm-II Reverse Transcription System (Promega), following the manufacturer′s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... and processed with the ImProm-II Reverse Transcription System (Promega) for cDNA synthesis ...