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Citations for Promega :
1201 - 1250 of 3904 citations for 6 7 Dihydro 2 formyl 5H pyrrolo 1 2 a imidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 řC ...
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bioRxiv - Genetics 2019Quote: ... each well was transfected with 2.1 ul FuGENE 6 (Promega), 600 ng of the mutant SCN5A plasmid and 100 ng of a plasmid expressing Bxb1 recombinase (a gift from Pawel Pelczar,21 Addgene plasmid #51271) ...
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bioRxiv - Microbiology 2022Quote: ... and gL using 2.5 μL/well FuGENE 6 (Promega, USA) in 500 μL/well DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... and 6 ng of Renilla luciferase vector (Promega, Cat. E6921) were co-transfected into HEK 293 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mESCs were transfected using the Fugene 6 Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were transfected using FuGENE 6 transfection reagent (Promega). Transfection mixture was prepared in serum-reduced OptiMEM medium (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... 6 hour digestion (both enzymes from Promega, 200 ng each). Samples were equilibrated to 0.1 % TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... HUH7 cells were transfected with FuGene 6 (Promega, Madison WI), and cell pellets were harvested 40 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by addition of 6 μL of FuGene HD (Promega). This entire mix was dripped onto the culture and incubated without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg total RNA was treated with DNase RQ1 (Promega), purified with RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Biochemistry 2019Quote: ... The beads were again washed with 50 mM ammonium bicarbonate buffer and resuspended in 100 µl of fresh 50 mM ammonium bicarbonate buffer and treated with 2 µg of sequencing grade modified trypsin (Promega; Product code V5113) at 37 oC overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA samples (2 μg) were used for the synthesis of cDNA by reverse transcription using GoScript ™ Reverse Transcriptase (Promega, Madison, USA). RT-PCR was performed with a CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: Hela cells were transfected with 2 µg of HaloTag® fusion construct and 20 ng NanoLuc® fusion construct using Fugene® HD (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 105 U2OS cells were transfected with 2 µg of sgRNA and Cas9 expressing plasmid using Fugene HD reagent (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used in this study were as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Celltiter 96 Aqueous One-solution Cell Proliferation Assay, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The wounded area and its surrounding area (2 x 2 cm) were immediately dissected out and RNA extraction was conducted using Maxwell® RSC simplyRNA Tissue Kit (AS1340, Promega, Madison WI) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg of RNA were used for reverse transcription into complementary DNA (cDNA) using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Affinity purified proteins retained on beads were resuspended in 100 µL of NH4HCO3 at 25 mM containing 2 µg of Trypsin/Lys-C mix (Mass Spec Grade, Promega, Madison, WI, USA). Digestion was performed under agitation at 37°C during 4h ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Molecular Biology 2020Quote: Apoptosis assays were performed using Caspase-Glo 3/7 reagent according to manufacturer’s instructions (Promega #0000239042). Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700) ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase activation was measured using the Caspase Glo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... cell metabolic activity or caspase 3/7 activity was measured using the CellTiter-Glo assay (Promega) and Caspase-Glo 3/7 assay (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... apoptosis was quantified using the Apo-ONE homogeneous caspase-3/7 assay kit (Promega, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Caspase activity was determined using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation was evaluated 7 days post-plating using CellTiter-Glo® Luminescent Assay (Promega, #G7570) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Caspase 3/7 activity was measured using the Caspase-Glo assay kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity and cell viability were determined by ApoTox-Glo assay kit (G6321, Promega). Briefly ...
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bioRxiv - Genomics 2023Quote: ... This TP53 response element is controlling the GADD45A gene (7) and is commercially available (pGL4.38, Promega). The benchmark response element was also linked to the three selected spacer sequences and the two core promoters ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were transfected on DIV 7-9 utilizing a Calcium phosphate transfection kit (Promega; Cat# E1200): In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and −7 activity was measured following three days of treatment (Caspase-Glo #G8093, Promega), while cell viability was obtained after six days of treatment (CellTiter-Glo 3D #G9681) ...
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... 24h prior to imaging cells were co-transfected with human PEX31-42-GFP-Halo and either human HAP1-mCherry-eDHFR or mouse BICD21-572-mCherry-eDHFR using FuGENE 6 (Promega; 1 µg total DNA) and 48h prior to imaging with control siRNA using Lipofectamine RNAiMAX (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... After two low stringency washes for 5 minutes each with 2× saline sodium citrate/ 0.1% sodium dodecyl sulfate buffer (2x SSC buffer; Promega Co., Madison, WI, Catalog# V4261) and a high stringency wash for 2 min with 1x SSC buffer ...
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bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2021Quote: Plasmids were transiently transfected into 293T17 cells using FuGENE 6 (Promega). For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites ...
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bioRxiv - Biochemistry 2019Quote: ... Transfection of plasmids into cells were performed using FuGENE 6 (Promega) according to the manufacturer’s protocol and the cells were harvested 24 hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were transfected with EB3-GFP using FuGENE 6 (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was transfected with 3 uL FuGENE 6 reagent (Promega) and 130 ng pcDNA3-mouseSTING in line with manufacturer’s protocols.
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bioRxiv - Cancer Biology 2021Quote: ... All transfections were performed with FuGENE 6 transfection agent (Promega E2691) in Opti- MEM Reduced Serum Medium (Gibco 31985070 ...
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bioRxiv - Biophysics 2020Quote: ... Transfection was performed using Fugene 6 transfection reagent (Promega, Madison, WI). When WT and mutant myc-KCNQ1 were co-transfected ...
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bioRxiv - Cancer Biology 2021Quote: ... expression plasmids were delivered with Lipofectamine 3000 or FuGene 6 (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... a total 3μg of plasmid DNA and 6μL FuGENE 6 (Promega) were combined in 300μL Opti-MEM (Gibco) ...