Labshake search
Citations for Promega :
1151 - 1200 of 3904 citations for 6 7 Dihydro 2 formyl 5H pyrrolo 1 2 a imidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
-
bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
-
bioRxiv - Microbiology 2022Quote: ... The light production was then monitored at 20-minute intervals at 37 °C for 2 hours using the Glomax 20/20 Luminometer (Promega, Madison, WI). A final measurement was also taken after the samples had been allowed to incubate overnight at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Filters were transferred to 2 mL microfuge tubes for further processing using the Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation) with a modified version of the kit protocol ...
-
bioRxiv - Microbiology 2020Quote: ... for gram-negative bacteria and cDNA library preparation was performed using the GoTaq® 2-Step RT-qPCR System (A6010, Promega, USA). The RT-PCR was done using the Applied Biosystems™ 7500 Real-Time PCR System and primers shown in the supplementary data table S2 (35-38) ...
-
bioRxiv - Pathology 2020Quote: ... RNA (2 µg) was reverse transcribed through the GoScript™ Reverse Transcription System performed with Oligo(dT)15 (Promega, Madison, Wisconsin, EUA), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... OXA-111-NcoI F or OXA-71-NcoI F and OXA-66-XhoI R primers (Table 2) and TA cloned into the vector pGEM-T Easy (Promega, United Kingdom). The inserts were confirmed by sequencing with the universal T7 Promoter primer ...
-
bioRxiv - Genomics 2019Quote: ... All PCR products were checked on 2% agarose gels and then cleaned with the Wizard SV Gel and PCR Clean-up System from Promega (Madison, Wisconsin) and sent off for Sanger-sequencing along with the amplification primers to Molecular Cloning Laboratories (San Francisco ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cell viability was assessed by the viability assays 3-(4,5-dimetiltiazol-2-il)-5-(3-carboximetoxifenil)-2-(4-sulfofenil)-2H-tetrazolio (MTS) using the Cell-Titer 96c AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, USA) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Luminescence was read after 2 minutes shaking on an orbital shaker and 10 min incubation at RT using the GloMax instrument (Promega, Madison, USA).
-
bioRxiv - Immunology 2021Quote: The effect of neutralization capacity of Multivalent DARPin was evaluated by exposing serial dilutions of the DARPin candidates to increasing titers of SARS-CoV-2 and determining cell protection by CellTiter-Glo assay (Promega, Madison, USA). Serial dilution of DARPin candidates were prepared in 96 well plates in 100 µl cell culture medium (2%-FBS-MEM + HSA ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
-
bioRxiv - Biophysics 2020Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA with oligo (dT)15 primers and GoScript™ Reverse Transcriptase (Promega, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR products were extracted from a 2% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified by QuantiFluor™-ST (Promega, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed 2 times with phosphate-buffered saline (PBS) and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega, Madison, WI). Using the Nano-Glo Luciferase Assay System (Promega ...
-
bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
-
bioRxiv - Developmental Biology 2023Quote: ... Table S2) were cloned into the XhoI/NotI sites downstream of the synthetic Renilla luciferase gene of psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... Caspase activity was determined using Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI) according to the manufacturer’s specifications ...
-
bioRxiv - Developmental Biology 2019Quote: ... The Promega Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI, Cat No. G8091) was used to quantify apoptotic cell death
-
bioRxiv - Molecular Biology 2019Quote: ... 7 and 9 h post transfection and luminescence was measured with luciferase assay reagent (Promega) using a Glomax luminometer (Promega).
-
bioRxiv - Microbiology 2021Quote: ... caspase activity was measured using Caspase-Glo 3/7 Assay according to manufacturer’s instructions (Promega). Staurosporine (1μM ...
-
bioRxiv - Neuroscience 2020Quote: Caspase activity was measured using a chemiluminescent assay kit (Promega Caspase-Glo® 3/7) in 96-well format using an Analyst HT plate reader (Molecular Devices ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated for 24 hr before adding 40 μl of Caspase-Glo 3/7 (Promega) to each well ...
-
bioRxiv - Cancer Biology 2021Quote: ... The remaining cells in the inserts were analyzed with Caspase-Glo 3/7 assay (Promega) to confirm that Jurkat cells incubated with TAM CM did not induce apoptosis compared to the control medium.
-
bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Caspase activation was measured using the CaspaseGlo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions for the indicated time point presented in the figure legend ...
-
bioRxiv - Systems Biology 2023Quote: ... genes were cloned in pFN19A (HaloTag®7) T7 SP6 Flexi® vector (Promega, USA) by Gibson assembly (Gibson et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... Apoptosis was assessed by commercially available caspase 3/7 assay (Cat # G8090, Promega, Madison, WI) as per the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: GP5d clonal cells (7 × 10E5) expressing the HiBiT-KRAS were transfected with FuGENE HD (Promega) and 1 μg of HaloTag-VHL ...
-
bioRxiv - Cell Biology 2021Quote: ... per 10 cm dish using Fugene 6 (Catalog# Promega# E2691), and the medium was changed after 8 hours using (IMEDM+10%FBS) ...
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were digested with 6 ng/µl trypsin (Promega, UK) overnight at 37°C ...
-
bioRxiv - Biochemistry 2019Quote: 293T cGAS ENPP1−/− cells were transfected with Fugene 6 (Promega) according to manufacturer’s instructions plus indicated concentrations of pcDNA3 plasmid DNA (empty or containing human ENPP1) ...
-
Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... 1.5 μl of Fugene 6 transfection reagent (Promega, Madison, WI) and 100 μl of OptiMEM (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... Plasmids were forward transfected into cells using FuGENE 6 (Promega). BEZ-235 ...
-
bioRxiv - Cell Biology 2019Quote: ... Plasmid transfections were performed with FuGENE 6 (Promega, cat#E2691) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Retroviral particles were generated using the Fugene 6 reagent (Promega) to simultaneously transfect subconfluent monolayers of 293T cells with 1μg pBABE (vector ...
-
bioRxiv - Cell Biology 2021Quote: Plasmid and siRNA transfections were done with Fugene 6 (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected using FuGENE 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: Transfections were performed with Fugene 6 transfection reagent (Promega, USA) according to manufacturer’s instructions with minor modifications ...
-
bioRxiv - Microbiology 2020Quote: ... and 0.25 μl of the transfection reagent Fugene 6 (Promega). After 20 min incubation at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... and luciferase-IRES-ZsGreen using FuGENE 6 Transfection Reagent (Promega). Culture supernatants were exchanged with fresh medium (high glucose DMEM supplemented with 2 mM L-glutamine ...
-
bioRxiv - Cell Biology 2019Quote: ... ‘ΔGsix’ HEK 293 cells were transfected using Fugene 6 (Promega) according to manufacturer specifications.
-
bioRxiv - Biochemistry 2021Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 °C ...
-
bioRxiv - Biophysics 2019Quote: ... mixed with 6 μL of Fugene HD transfection reagent (Promega) and incubated for 15 minutes at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were transfected using the FuGENE 6 Transfection Reagent (Promega) at a ratio of 1 μg DNA/3 μL FuGENE 6 according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were transfected with cDNA using FuGene 6 (Promega, E2691) following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega ...
-
bioRxiv - Cell Biology 2021Quote: ... 1.5 μl of Fugene 6 transfection reagent (Promega, Madison, WI) and 100 μl of OptiMEM (ThermoFisher Scientific ...