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Citations for Promega :
1151 - 1200 of 4040 citations for trans 6 Chloro 1 hexen 1 ylboronic acid pinacol ester 96% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated antibody (1:2000; Promega, Madison, WI, USA) and washed again with TBS-T ...
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bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... clarified by centrifugation (500 x g, 5 min, at 15°C) and mixed 1:1 with 2× passive lysis buffer (Promega cat #E1941). Cell monolayers were washed in PBS and lysed in 1× passive lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding different Ocrl1 mutants were transfected using Fugene 6 reagent (Promega) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: DNA was transiently transfected into cells using FuGENE 6 transfection reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1,3 μg of plasmid DNA and 3 μl of FuGene 6 (Promega) were mixed in 100 µl Opti-MEMTM medium before the addition to the dish ...
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bioRxiv - Microbiology 2022Quote: All ectopic transfections were performed using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5-6ng pcDNA3.1 FLAG-cGAS and STING using 0.75μl FuGENE 6 (Promega) and 20μl Opti-MEM (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 1.2 µg p8.91 and 1.2 µg KAP1 pHRSIN using FuGENE 6 (Promega). Virus-containing supernatant was collected 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... Viral stocks were generated by transfecting 293T cells using Fugene 6 (Promega). After 48h ...
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bioRxiv - Microbiology 2022Quote: ... The DNA plasmid mix was added to 18µl of Fugene-6 (Promega) transfection reagent in 200µl of pre-warmed Opti-MEM (Gibco ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... Transfections and co-transfections were performed using FuGENE 6 transfection reagent (Promega). Cells were analysed two days post-transfection.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Fugene 6 reagent (Promega) by following the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: RSV VLPs were produced by FuGENE® 6 Transfection Reagent (Promega, E2691) transfection of DF1s at 50% confluence with 1 µg of viral plasmid ...
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bioRxiv - Immunology 2021Quote: ... and HIV Rev plasmid using Fugene 6 transfection reagent (Promega, Madison, WI). Virus-containing medium was titrated to ensure undersaturating conditions for infection of H1299 cells ...
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bioRxiv - Cell Biology 2022Quote: ... For transient transfection cells were incubated with mixture of FuGene 6 (Promega) and the plasmid of interest ...
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bioRxiv - Biochemistry 2022Quote: ... was mixed with 10 μL of FuGENE 6 transfection reagent (Promega E2693). After 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (70% confluent) were transfected using FuGene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the plasmid of interest using Fugene 6 transfection reagent (Promega). All shRNA plasmids used were purchased from Sigma ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE® 6 transfection reagent (Promega). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega). After overnight incubation at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using a master mix of Fugene 6 (Promega, E269A) and Optimem (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were transfected with 3 μL of FuGENE 6 (Promega) and 1 μg pA2 plasmid DNA per well of a six well plate ...
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bioRxiv - Immunology 2024Quote: ... These three plasmids were cotransfected into HEK293T cells using Fugene 6 (Promega). Media was exchanged after three days and ultimately collected after five days total then passed through a 0.45μm syringe filter before being stored at -80C.
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were transfected using 3 μL of FuGENE 6 (Promega) and 0.5 μg Alu reporter plasmid + 0.5 μg TMO2F3 plasmid DNA per well of a six well plate as noted above ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were grown to 80% confluence and transfected using Fugene 6 (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... they were transfected with indicated plasmids using FuGENE 6 transfection reagent (Promega). After another 24h ...
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bioRxiv - Biophysics 2024Quote: ... After 6 to 24 hours cells were transfected using Viafect reagent (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... 0.3% xylose and 1% low melting temperature agarose (Promega) on slides ...
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bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). The mixture was subsequently incubated with 20 µL of Dynabeads protein-A (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). After the addition of 50 µL Dynabeads protein-A (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of RNAse inhibitor (40 U/µl, Promega) was added to stop the reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:1000) and b-actin-peroxidase (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:100). The following Alexa Fluor secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Biochemistry 2020Quote: ... 1x reaction buffer (from Promega (containing 1 mM Co2+) for Supplementary Fig ...
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bioRxiv - Bioengineering 2020Quote: ... and 1 unit T4 ligase with associated buffer (Promega). The following thermocycler program was run ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of streptavidin-coupled magnetic beads (Promega, Z5481) per condition were washed three times during 10 min at 4°C (under intermittent shaking ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µg of sequencing-grade modified trypsin (Promega, USA) was added to each solution ...
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bioRxiv - Neuroscience 2020Quote: ... and Donkey anti-rabbit HRP (Promega W401B, 1:10,000). Soluble protein samples were normalized to REVERT total protein stain (Li-Cor).
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bioRxiv - Cell Biology 2021Quote: ... with freshly added Halt protease inhibitor (1:100, Promega)) ...