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Citations for Promega :
1051 - 1100 of 4040 citations for trans 6 Chloro 1 hexen 1 ylboronic acid pinacol ester 96% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... followed by a 1:20 trypsin (V511A, Promega) to protein concentration for overnight digestion (16 hours) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg RNA was treated with DNase (Promega) and cDNA was synthesised via incubation with random hexamer primer (C1181 ...
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bioRxiv - Cancer Biology 2021Quote: Plasmids were transiently transfected into 293T17 cells using FuGENE 6 (Promega). For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 µl/well of FuGENE 6 Transfection reagent (Promega; E2691) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 µl/well of FuGENE 6 Transfection reagent (Promega; E2691) following the manufacture’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was transfected with 3 uL FuGENE 6 reagent (Promega) and 130 ng pcDNA3-mouseSTING in line with manufacturer’s protocols.
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bioRxiv - Cancer Biology 2021Quote: ... All transfections were performed with FuGENE 6 transfection agent (Promega E2691) in Opti- MEM Reduced Serum Medium (Gibco 31985070 ...
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bioRxiv - Biophysics 2020Quote: ... Transfection was performed using Fugene 6 transfection reagent (Promega, Madison, WI). When WT and mutant myc-KCNQ1 were co-transfected ...
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bioRxiv - Cancer Biology 2021Quote: ... expression plasmids were delivered with Lipofectamine 3000 or FuGene 6 (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... a total 3μg of plasmid DNA and 6μL FuGENE 6 (Promega) were combined in 300μL Opti-MEM (Gibco) ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with 6 μM EnduRen (Promega, Madison, WI, USA), a substrate for Renilla luciferase ...
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bioRxiv - Biophysics 2021Quote: pGFP was transfected using FuGENE®6 Transfection Reagent (E2691,Promega) with OptiMEM (31985088 ...
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bioRxiv - Immunology 2022Quote: ... Phoenix cells were transfected using FuGENE 6 Transfection Reagent (Promega #E2691), and retroviral supernatant was collected and used immediately for transfection of MC38s on days 2 and 3 following transfection ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.4 ng plasmid encoding Renilla luciferase using FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection using the Dual Luciferase assay kit (Promega ...
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bioRxiv - Cancer Biology 2022Quote: Transfections were carried out using FuGENE 6 transfection reagent (Promega #E2691) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and target (400 ng) plasmids using 6 μL FuGene HD (Promega). 72 hrs later ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2.7 μl FuGENE 6 Transfection Reagent (E2691, Promega, Madison, WI) in 1.5 ml volume ...
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bioRxiv - Physiology 2021Quote: ... and 5 μg lentiviral construct with 30 μL Fugene 6 (Promega) the following day ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were mixed with Fugene 6 transfection reagent (Promega, Madison, WI) at a dilution of 1:12 in a total volume of 600 μl ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids were transfected into HeLa cells using Fugene 6 (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg VSV-G envelope plasmid using Fugene 6 (Promega) transfection reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2.25 μg psPax2) and 18 μL of FuGENE 6 (Promega). The culture medium was changed 6-24 h later ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufactureŕs protocol.
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfection was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... then transfected with 1.5 µg plasmid DNA using FuGENE 6 (Promega) as follows ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected into C2C12 cells using a Fugene 6 reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with the help of Fugene 6 (Promega) using a ratio of 3:1 of reagent to DNA ...
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bioRxiv - Cell Biology 2023Quote: Cells expressing one plasmid were transfected with FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the indicated plasmids using FuGENE 6 (Promega) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... then digested by treatment with 6 µl of Trypsin Gold (Promega). Peptides were extracted and concentrated for MS analysis.
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Biophysics 2021Quote: ... IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Microbiology 2023Quote: The assay was adapted from 15 where Hyp1-Nluc schizonts at 1% hematocrit and 1-2% parasitaemia were lysed in 1x NanoGlo buffer (Promega, USA) within a 96-well flat-bottom plate and parasite lysates were added to compounds and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 1% paraformaldehyde/PBS for 1 minute and washed with PBS and with 2X SSC (Promega, V4261), followed by Incubation in 50%formamide/2X SSC (pH7-7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
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bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were washed with Wash Buffer three more times and nuclei pellets were resuspended in Diluted Nuclei Buffer (1x Nuclei Buffer, 10x genomicsTM, supplemented with 1 mM DTT and 1 U/uL RnaseIn, Promega©) for counting and scMulti library preparation ...
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bioRxiv - Cell Biology 2024Quote: ... Islets were fixed in paraformaldehyde (4%) and blocked without permeabilization in 1% BSA prior to 48-hour incubation with anti-Halo antibody (1:500; Promega, UK) in 0.1% BSA/PBS at 4°C ...