Labshake search
Citations for Promega :
1151 - 1200 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... In-gel protein digestion was performed overnight at 37°C with trypsin (Sequencing Grade Modified Trypsin, Promega, Madison, USA) at a final concentration of 0.005 µg/µL ...
-
bioRxiv - Genetics 2021Quote: ... The samples were reconstituted in 50 µL of 20% acetonitrile 80 mM triethyl ammonium bicarbonate (TEAB) and digested with 3.3 µg of trypsin/Lys-C mix (Promega) at 37C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... The sample was first treated by 2.5 μg of LysC (Wako) for 3 h at 37 °C with shaking and then treated with 2.5 μg of Trypsin (Promega) for over-night at 37 °C with shaking ...
-
bioRxiv - Biochemistry 2021Quote: ... Reactions were then incubated at 37 °C for 2 min before the addition of 30 units of SmaI (Promega), 46 kBq [a-32P]-dCTP (111 TBq/mmol ...
-
bioRxiv - Neuroscience 2021Quote: ... equilibrated at 20°C for 30 minutes and 30 μl CellTiter-Glo Luminescent Cell Viability Assay reagent (Promega, #G7573) was distributed into each well ...
-
bioRxiv - Biophysics 2021Quote: ... the C terminus of MC1R was fused with a 15-amino-acid polypeptide linker (GSSGGGGSGGGGSSG) and a LgBiT (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
-
bioRxiv - Neuroscience 2021Quote: ... all DNA constructs were prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
-
bioRxiv - Microbiology 2020Quote: ... was then diluted into fresh 50mM ammonium bicarbonate and incubated overnight at 37°C either with 0.056 μg of trypsin (Promega), chymotrypsin (Sigma Aldrich ...
-
bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
-
bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
-
bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
-
bioRxiv - Developmental Biology 2024Quote: ... Washes were performed 6x with 200 µL each and digestion was performed with 0.75 µg Trypsin/Lys-C Mix (Promega) per 10 µg total protein ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were digested for 2 hr at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega), and overnight at 25°C with proteomics-grade porcine trypsin (T6567 ...
-
bioRxiv - Systems Biology 2023Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
-
bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
-
bioRxiv - Systems Biology 2023Quote: ... Plates were incubated at 37°C for 96 hours and lysed using CellTiter-Glo Reagent (Promega, catalog. no. G7570). Luminescence was measured using a PHERAstar FS instrument and growth inhibition was calculated relative to DMSO-treated wells.
-
bioRxiv - Biochemistry 2023Quote: ... samples were washed twice with S-Trap buffer before overnight digestion at 37° C with Trypsin (Promega Cat# V511X) in digestion buffer (50 mM ammonium carbonate ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
-
bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
-
bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
-
bioRxiv - Pathology 2023Quote: ... Overnight digestion was performed at 37 °C with 2 μL of freshly prepared trypsin at 0.1 μg/μl (V5111, Promega) in 50 mM ammonium bicarbonate (per 100 μl of sample) ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37°C overnight before luciferase activity was quantified using the Luciferase Assay System (Promega) on SoftMax Pro v.7.0.2 (Molecular Devices) ...
-
bioRxiv - Microbiology 2023Quote: ... OD600nm was monitored every hour at 37°C for 24 h by using a GloMax Explorer plate-reader (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
-
bioRxiv - Genomics 2024Quote: ... The column was washed twice with 150 µL of loading buffer and gently centrifuged before overnight digestion at 37°C with 0.1 µg/µL of trypsin (Promega) in 50 mM ammonium bicarbonate ...
-
bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were then divided in two to obtain tryptic and Glu-C peptides and diluted to final concentration of 0.1 mg/mL with 50 mM NH4HCO3 for Trypsin digestion and with PBS for Glu-C (Promega) digestion ...
-
bioRxiv - Cell Biology 2019Quote: ... diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega). We visualized and quantified 5-FUrd incorporation by using high content imaging device (OPERETTA ...
-
bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
-
bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...
-
bioRxiv - Microbiology 2019Quote: A Luciferase Assay System kit (Promega) was used ...
-
bioRxiv - Plant Biology 2019Quote: The Reverse Transcription System Kit (Promega) was used to synthesize a cDNA library following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2020Quote: ... the PureYield™ MidiPrep Kit (ProMega) and NucleoBond Xtra™ Midi/Maxi Kit (Macherey & Nagel ...
-
bioRxiv - Genetics 2021Quote: ... using the Renilla Glo Kit (Promega) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... the Glutamate Glo Assay Kit (Promega) was used ...
-
bioRxiv - Microbiology 2021Quote: ... the Glucose Uptake-Glo kit (Promega) was used ...
-
bioRxiv - Microbiology 2022Quote: ... The T7 RiboMax express kit (Promega) was used for the in vitro production of 16S rRNA under standard conditions.
-
bioRxiv - Molecular Biology 2019Quote: ... using QuantiFluor ONE dsDNA kit (Promega) and library size was checked on the Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase or Renilla activity (Galactostar ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the dual luciferase kit (Promega). Experiments were done at least in triplicate ...
-
bioRxiv - Biochemistry 2020Quote: The “ADP-Glo” kit (Promega, Wisconsin) was used to measure hydrolysis of ATP to ADP and inorganic phosphate by ATPase ...
-
bioRxiv - Bioengineering 2020Quote: ... Steady-Go luciferase assay kit (Promega) was used to measure the expression of luciferase in cells ...