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Citations for Promega :
1351 - 1400 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: The protein lanes were excised from the gels and proteolytic digestion with trypsin/lys-C mix (V5073, Promega, Southampton, United Kingdom) was performed as described (Shevchenko et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were diluted 10-fold with 100 mM Tris (pH 8.0) and incubated overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1h ...
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bioRxiv - Systems Biology 2019Quote: ... lysates were diluted in 700µl of TBS and incubated at 4°C with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... Bound fractions were then washed with 1xPBS to remove excess salt followed by RQ1 DNase treatment at 37° C for 10 minutes [4 units of RQ1 (Promega M6101) and 60 units of SUPERaseIN (ThermoFisher AM2694)] ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
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bioRxiv - Immunology 2021Quote: ... The indicated compounds were introduced analytically using a CyBio pintool and the plates left to incubate at 37 °C prior to the addition of the CellTiter-Glo reagent (Promega, G7573) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... The dried gels were incubated for 16 h at 37°C in 50 mM ammonium bicarbonate containing sequence grade modified trypsin (Trypsin Gold; Promega, USA). The digested peptides were eluted from the gels with 50% acetonitrile (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Digestion solutions were brought to a final concentration of 2 M urea and 2 mM CaCl2 and a second digestion was performed overnight at 37 °C using trypsin (Promega, v5280) at 1:100 w/w ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant C-terminally HA tagged Su(H) was produced using in vitro coupled transcription/translation (TNT with rabbit reticulocyte extracts; Promega Inc.) from DNA matrices generated by PCR using Su(H)-HA forward and reverse primers combined with the Su(H ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were dried in a vacuum centrifuge and resuspended in 200 uL of 0.05 ug/uL Lys-C/Trypsin (Promega, WI, USA) in 25 mM Ammonium Bicarbonate ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40 °C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40 °C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 40 °C ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... The resulting protein extract was first diluted to 2M urea for overnight digestion with LysC (Wako, USA) at 37°C and then diluted 2-fold for 8 h digestion with trypsin (Promega, USA) at 37°C.
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bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
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bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40°C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40°C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 hours at 40°C ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Biophysics 2023Quote: ... each respective cell type was incubated at 37 °C with 500 pM of Janelia Fluor® 646 HaloTag Ligand (NSCs, ECs, or keratinocytes) (Cat. No. GA1120, Promega) or Janelia Fluor® 635 HaloTag Ligand (MNRs ...
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bioRxiv - Molecular Biology 2023Quote: ... and MSN(T558A) genes with either N-terminal or C-terminal SmBit and LgBiT fusions were produced using Flexi®-based cloning (Promega). Best luminescence signals were obtained with CD44 containing a C-terminal smBiT fusion (C-terminal smBiT ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.
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bioRxiv - Immunology 2022Quote: ... The samples were subsequently quenched with 10 mM DTT with overnight digestion at 37°C using Trypsin gold (Promega, WI, USA). The digestion was terminated and the surfactant was cleaved by treating the samples with 200 mM HCl (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Beads were incubated overnight at 37°C with 20 μL of 25 mM NH4HCO3 buffer containing 0.2µg of sequencing-grade trypsin (Promega, Madison, WI, USA). The resulting peptides were loaded and desalted on evotips provided by Evosep (Odense ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... The well plates were incubated at 30 °C and the optical density at 560 nm was measured using a plate reader (Promega ProMax). We defined a growth inhibition of at least 50 % in comparison to the growth in the DMSO-supplemented medium as the cut-off to determine the MIC.
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bioRxiv - Microbiology 2024Quote: Proteins were digested for 11 hours at 37°C under agitation at 600 rpm on a ThermoMix (Thermo) using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Developmental Biology 2021Quote: ... anti-β-Galactosidase (1:1.000; Promega, 1:1000; abcam), anti-Cleaved Caspase-3 (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...