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Citations for Promega :
1101 - 1150 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HPR-conjugated secondary antibodies were from Promega. Anti-Mouse Alexa Fluor 488 (A32790) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HRP conjugated secondary antibodies were from Promega. Anti-mouse Alexa® Fluor 488 (#A32790 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were detected with an anti-mouse secondary antibody conjugated to HRP (Promega) at 1:5000 dilution in 5% fat-free milk ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Anti-mouse and anti-rabbit Abs conjugated with horseradish peroxidase (HRP) were from Promega Biotec (Madison ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (0.5 µg) was reverse transcribed with mouse myeloblastosis virus reverse transcriptase (Promega) under standard conditions using hexanucleotide random primers according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The mouse Il12b promoter (−355 to +55) was cloned into the pGL4.10 vector (Promega). Motif mutations were introduced using the GENEART site-directed mutagenesis system (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... but the secondary antibodies were goat anti-mouse HRP conjugated antibody (Promega Cat #W4021) and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011) ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Urea was reduced to 1 M by addition of 500 μl AB and overnight digestion was performed at 37 °C with 5 μl of freshly prepared 0.2 μg/μl trypsin (Promega) in AB ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Urea-solubilized pellet was then diluted to 2M urea using NaCl/Tris solution (150mM NaCl, 50mM Tris pH 8.0) and digested overnight at 37°C with 10ug of trypsin GOLD (Promega). The following day ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: Proteins were digested overnight at 37°C in 20 ml of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 mg/ml; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile and 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Cancer Biology 2019Quote: ... was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL) (Promega) for an additional 20 min incubation at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Systems Biology 2019Quote: ... with N-and C-terminus tagging configurations (NB MCS1, NB MCS2, NB MCS3, NB MCS4) were obtained from Promega, each containing an HSV-TK promoter for protein expression in mammalian cells and an ampicillin resistance marker for bacterial selection of transformed cells ...
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bioRxiv - Cancer Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Finally ...
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bioRxiv - Cancer Biology 2019Quote: ... and cultured for 72 h at 37°C before viability assay using the ATP-based CellTiter-Glo (CTG) 2.0 Assay (Promega). Luminescence was read with the LMAX II luminometer (Molecular Devices) ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then incubated overnight at 37 °C with 500 ng Sequencing Grade Modified Trypsin (Promega, Madison, WI, USA). To achieve the complete digestion of the peptides ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated at 37 °C overnight and the luciferase activity was measured using the Bio-Glo Luciferase Assay System (Promega) on the EnVision Multilabel Plate Reader (Perkin Elmer) ...
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bioRxiv - Neuroscience 2020Quote: ... in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega) per sample in 100 mM ABC without 0.1% SDC ...
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bioRxiv - Microbiology 2020Quote: ... Urea was reduced to 1 M by addition of 500 μl AB and overnight digestion was performed at 37 °C with 5 μl of freshly prepared 0.2 μg/μl trypsin (Promega) in AB ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 0.2 mg of protein was subjected to overnight digestion with 8.0 µg of mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Plant Biology 2020Quote: ... Tryptic in-gel digestion of gel slices was performed overnight at 37°C in 50 mM ammonium bicarbonate using 0.1 µg trypsin (Promega) per gel band ...
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bioRxiv - Neuroscience 2020Quote: Plates were incubated for 24 hours at 37°C and assayed for FL and RL using the Dual-Luciferase Assay System and following manufacturer’s instructions (E1980, Promega). Liquid handling and luciferase assay were performed using the integrated automated screening platform (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Urea was reduced to 1 M by addition of 500 ml AB and overnight digestion was performed at 37°C with 5 ml of freshly prepared 0.2 mg/ml trypsin (Promega) in AB ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C/5% CO2 for 3 days before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega). Luminescence was read using a Molecular Devices Spectramax L plate reader ...
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bioRxiv - Microbiology 2020Quote: ... with the required amount of cells (cell number equal to the number of wells multiplied by 1.5 × 104).The effector and target cells were mixed 20 h post-transfection at 37°C in fresh medium containing 60μMmembrane-permeable substrate for Renilla luciferase (RL) (Enduren Live Cell Substrate; Promega)1 h prior to mixing ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 72hr at 37°C with 5% CO2 and then measured by CellTiter-Glo assay (Promega). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... before the addition of digestion buffer (50 mm Tris) containing 0.8 μg Lys-C (Wako, 129–02541) and 0.8 μg Trypsin-gold (Promega, V5280). Enzymatic digestions proceeded under agitation (400 rpm ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were diluted with 900 µl of 25 mM ammonium bicarbonate and digested overnight at 37°C in the presence of 0.35 µg of sequencing grade modified porcine trypsin (Promega). Peptides were vacuum-dried in a centrifugal evaporator to approximately 250 µl ...
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bioRxiv - Microbiology 2022Quote: ... HEV) or at 33°C (HRV) until harvested by washing in PBS and lysis in Passive Lysis Buffer (Promega). Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium) ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at 30° C in the dark and finally tryptically digested with 1.25 µg of sequencing grade porcine trypsin (Promega) in 100 mM ammonium bicarbonate at 37° C for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... alkylated with 100 mM iodoacetamide and proteolytically cleaved over night at 37°C using trypsin (Promega, Madison, WI, USA). Peptides were extracted ...
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bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein pellets were resuspended in 40μl of 100mM TEAB and digested overnight at 37°C with shaking by the addition of 2μl of 1μg/μl trypsin (Promega, V5111) and 1μl of 80mM CAM (2mM final ...
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bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads were finally added with 2.5 μl of 200 ng/µl Trypsin/Lys-C Mix (Cat. No. V5073, Promega) at 37°C with shaking overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was stored at −20°C until analysed by polymerase chain reaction using GoTaq®G2 DNA polymerase (Promega) according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 54°C for 10 min and the resulting cDNA amplified with GoTaq Flexi DNA polymerase (Promega; Madison, WI) for 8 PCR cycles in the same reaction with a final volume of 100 μL ...
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bioRxiv - Cell Biology 2019Quote: Proteins were then digested overnight at 37°C in 20 μL of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 μg/mL; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile ...
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...