Labshake search
Citations for Promega :
1151 - 1200 of 1751 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 1.5 µl of Fugene HD according to manufacturer’s instructions (Promega) for 40 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... The WT and mutated 5’UTR sequences were ligated into a linearized reporter plasmid (psiCHECK-2 vector, Promega). The entire sequences of all the plasmids were validated by Sanger sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: ... CDC20 and ANAPC4 knockouts were obtained with sgRNA plasmids transfected using FuGENE® HD Transfection Reagent (Promega, E2311), followed by selection using puromycin (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... The HdAd plasmid was linearized with PmeI and then transfected (Profection Mammalian Transfection System, Promega, Madison, WI, USA) into 116 producer cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen, UK and Promega, UK, respectively) following manufacturers’ instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The two plasmids were transfected at a ratio of 20 ng:20 ng per well using ViaFect (Promega), employing a transfection agent to plasmid ratio of 3:1 μL:μg.
-
bioRxiv - Microbiology 2023Quote: Transfection of DNA plasmids into different mammalian cells was conducted using FuGENE® HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc fusion (Promega) as specified in Table S3 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 25 ng SRE-Luc reporter (E134A, Promega), 75 ng Gαi or Gαi QL in pcDNA3.1+ ...
-
bioRxiv - Cell Biology 2023Quote: Immortalized DMDΔ52 myoblasts were co-transfected with 1.5ug of each plasmid containing the selected sgRNAs using ViafectTM (Promega) transfection reagent (1:5 ratio) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The Carm1-reporter or its mutated reporters was co-transfected into 293T with the pRL-TK plasmid (Promega) expressing renilla for normalization ...
-
bioRxiv - Genomics 2023Quote: ... cells were transfected with 1µg L1 plasmid per well using 3µL FuGENE HD transfection reagent (Promega, Cat# E2311) and 97µL Opti-MEM (Life Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... each well was transfected with 1 μg of plasmid DNA (0.5 μg L1 plasmid + 0.5 μg pCEP/GFP) using 4 μL of FuGENE 6 transfection reagent (Promega); the following day ...
-
bioRxiv - Cell Biology 2020Quote: ... and SacI/XhoI restriction site overhands were annealed then ligated into the dual luciferase reporter plasmid (Promega, Cat#E1960). For mutated sequences ...
-
bioRxiv - Cell Biology 2019Quote: HeLa cells were cultured on 12-well plates and transfected with the indicated plasmids using Viafect (Promega, Madison, WI). After expression for 24h ...
-
bioRxiv - Molecular Biology 2020Quote: pCDH-WSB1 and pGL4.14-c-Myc promoter-luciferase plasmids as well as pCDH-WSB1 and pGL4.14-TCF/LEF1-luciferase plasmids were transfected on H460 and tested with the Luciferase reporter kit (Promega) in the same way ...
-
bioRxiv - Biochemistry 2019Quote: ... Mutations were verified by sequencing (Macrogen) and DNA for transfection was purified using the PureYield Plasmid MaxiPrep System (Promega).
-
bioRxiv - Molecular Biology 2019Quote: ... were plated in T25 flasks and transiently transfected with 2.5 µg plasmid DNA per flask using FuGENE HD transfection reagent (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 μg of sgRNA construct and 1.5 μg of linearized HDR plasmid were transfected into RPE1 cells using Fugene 6 (Promega). Positive cells were FACS sorted to isolate the Ndc80-EGFP expressing cells and single clones were identified by visual inspection with fluorescent microscope ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293T cells in 12-well plates were co-transfected with 0.05 µg each of GLP-1R-SmBiT and βarr2-LgBiT plasmid (Promega) plus 0.9 µg pcDNA3.1 ...
-
bioRxiv - Plant Biology 2019Quote: ... PCR reactions with 50 ng plasmid template were performed in 50 µl volume containing 10x DreamTaq buffer and 2.5 units of DreamTaq polymerase (Promega). Standard PCR conditions were 94 °C for 5 min ...
-
bioRxiv - Bioengineering 2019Quote: ... Linearized plasmid DNA was purified using the Wizard® SV Gel and PCR Clean-up Kit (Promega, Madison, WI) in preparation for transfection into Jurkat cells.
-
Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
-
bioRxiv - Genomics 2019Quote: ... 120 ng of plasmid per well were transfected along with 30 ng of pGL4.74 Renilla luciferase expression vector (Promega), to correct for transfection efficiency ...
-
bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
-
bioRxiv - Microbiology 2020Quote: ... Transfection was performed using 100 ng of expression plasmid using 0.3 μl of FuGENE HD Transfection Reagent (Promega E2311) in 20 μl of Opti-MEM medium (Life Technologies) ...
-
bioRxiv - Biochemistry 2021Quote: The assay was performed as described previously.22,23 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table 6 ...
-
bioRxiv - Cancer Biology 2021Quote: ... reversely transfected into LNCaP or VCaP (treated with DHT or ETH) cells with a Renilla Luciferase control plasmid pGL4.75 [hRluc/CMV] (Promega) by using X-treme GENE HP DNA Transfection Reagent (Roche) ...
-
bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with linearized repair templates and the PX459 plasmid containing the appropriate guides using FuGENE HD (Promega) with a ratio of Reagent ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
-
bioRxiv - Biochemistry 2022Quote: ... a 20 mL suspension culture of Expi293 cells was co-transfected with a pCDNA3.1 plasmid expressing TSHR and a luciferase-based cAMP biosensor (pGlosensor-22F; Promega). A total of 20 µg of DNA was transfected using Expifectamine per manufacturer instructions in a 3:1 ratio of receptor construct to cAMP biosensor ...
-
bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: The S1PR1 3’UTR sequences and mutant sequences (200 bp upstream and 200 bp downstream of the binding site from NM_007901.5 transcript) were synthesized and cloned into wildtype plasmid pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, USA) by Generay (China) ...
-
bioRxiv - Cell Biology 2022Quote: ... and further transfections with siRNA resistant plasmids were performed 24 hours after siRNA treatment using FuGENE® HD (Promega).
-
bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
-
bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
-
bioRxiv - Developmental Biology 2020Quote: ... with 100 ng of firefly luciferase reporter p12xAT_luc or p12xAT_luc(Mut) (Hojo et al., 2016) 15 ng of the pRL-TK Renilla luciferase control plasmid (Promega) and 100ng (unless otherwise indicated ...
-
bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with YFP-FKBP-Luciferase and PACT-Ce3-FRB plasmids using Fugene HD transfection reagent (Promega) 20-24 hr before synchronization ...
-
bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into a T Easy plasmid using the pGEM®-T Easy Vector (Promega, USA), following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... cells were transfected with 1μg L1-mCherry or L1-mCherry_RT-plasmid per well using 3μL FuGENE HD transfection reagent (Promega, Cat#: E2311) and 97μL Opti-MEM (Life Technologies ...
-
bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
-
bioRxiv - Zoology 2020Quote: ... K562 cells were transfected with 0.1 μg of Renilla luciferase expression plasmid pRL-TK (internal control for normalizing transfection efficiency; Promega) and 0.4 μg of abcb4 promoter construct ...
-
bioRxiv - Biochemistry 2019Quote: ... pSGG containing the human Nrf2 3ʹ–UTR sequence (from Prof. Qun Zhou, University of Maryland School of Medicine)] and Renilla luciferase plasmid (pGL4.75, E693A (Promega)) in a 40:1 ratio for 48 h using TransIT 2020 (Mirus Bio ...
-
bioRxiv - Plant Biology 2021Quote: ... PCR reactions with 50 ng plasmid template were performed in 50 µl volume containing 10x DreamTaq buffer and 2.5 units of DreamTaq polymerase (Promega). Standard PCR conditions were 94 °C for 5 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The following amounts of DNA were used per well: 50 ng of pGloSensor™ −20F cAMP Plasmid (E1171, Promega), 125 ng of untagged Gαi1-WT ...